E 64d

Name: E 64d
Brand: Tocris Bioscience
SKU: 4545

Tocris Bioscience, part of Bio-Techne | Catalog # 4545

Citations (30)

COA / SDS

Cathepsin inhibitor; interferes with autolysosomal digestion

Key Product Details

Description:	Cathepsin inhibitor; interferes with autolysosomal digestion
Chemical Name:	(2S,3S)-3-[[[(1S)-3-Methyl-1-[[(3-methylbutyl)amino]carbonyl]butyl]amino]carbonyl]-2-oxiranecarboxylic acid ethyl ester
Purity:	≥98% (HPLC)
Molecular Weight:	342.43

View Full Technical Details for E 64d

Technical Data
Citations

Biological Activity for E 64d

E 64d is an inhibitor of cathepsins B and L; also thought to inhibit calpain. Inhibits lysosomal proteases and interferes with autolysosomal digestion when used in combination with pepstatin A (Cat. No. 1190). Lysosome and cell permeable. Also inhibits entry of SARS-Cov-2 (COVID-19) into Caco-2 and Vero cells in combination with Camostat mesylate (Cat.No. 3193) in vitro.

Technical Data for E 64d

M. Wt	342.43
Formula	C₁₇H₃₀N₂O₅
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	88321-09-9
PubChem ID	65663
InChi Key	SRVFFFJZQVENJC-IHRRRGAJSA-N
Smiles	O=[C@@](N[C@H]([C@](NCCC(C)C)=O)CC(C)C)[C@H]1O[C@@H]1C(OCC)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for E 64d

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	ethanol	6.85	20

Preparing Stock Solutions for E 64d

The following data is based on the product molecular weight 342.43.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.2 mM	14.60 mL	73.01 mL	146.02 mL
1 mM	2.92 mL	14.60 mL	29.20 mL
2 mM	1.46 mL	7.30 mL	14.60 mL
10 mM	0.29 mL	1.46 mL	2.92 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

References for E 64d

References are publications that support the biological activity of the product.

Hoffmann SARS-CoV-2 cell entry depends on ACE2 and TMPRSS2 and is blocked by a clinically-proven protease inhibitor. Cell 2020 PMID: 32142651
Wilcox and Mason Inhibition of cysteine proteinases in lysosomes and whole cells. Biochem.J. 1992 PMID: 1637341
Sato Autophagy is activated in colorectal cancer cells and contributes to the tolerance to nutrient deprivation. Cancer Res. 2007 PMID: 17942897
Tanada Lysosomal turnover, but not a cellular level, of endogenous LC3 is a marker for autophagy. Autophagy 2005 PMID: 16874052
Kim Autophagy upregulation by inhibitors of caspase-3 and mTOR enhances radiotherapy in a mouse model of lung cancer. Autophagy 2008 PMID: 18424912