E 64d
Tocris Bioscience, part of Bio-Techne | Catalog # 4545
Cathepsin inhibitor; interferes with autolysosomal digestion
Key Product Details
Description
Cathepsin inhibitor; interferes with autolysosomal digestion
Product Description
E 64d is an inhibitor of cathepsins B and L; also thought to inhibit calpain. Inhibits lysosomal proteases and interferes with autolysosomal digestion when used in combination with pepstatin A (Cat. No. 1190). Lysosome and cell permeable. Also inhibits entry of SARS-Cov-2 (COVID-19) into Caco-2 and Vero cells in combination with Camostat mesylate (Cat.No. 3193) in vitro.Product Specifications
Molecular Weight
342.43
Formula
C17H30N2O5
Storage
Store at -20°C
Purity
≥98% (HPLC)
Chemical Name
(2S,3S)-3-[[[(1S)-3-Methyl-1-[[(3-methylbutyl)amino]carbonyl]butyl]amino]carbonyl]-2-oxiranecarboxylic acid ethyl ester
CAS Number
88321-09-9
PubChem ID
65663
InChI Key
SRVFFFJZQVENJC-IHRRRGAJSA-N
SMILES
O=[C@@](N[C@H]([C@](NCCC(C)C)=O)CC(C)C)[C@H]1O[C@@H]1C(OCC)=O
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| Ethanol | 6.85 | 20 |
Preparing Stock Solutions
for E 64d
The following data is based on the product molecular weight 342.43
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.2 mM | 14.60 mL | 73.01 mL | 146.02 mL |
| 1 mM | 2.92 mL | 14.60 mL | 29.20 mL |
| 2 mM | 1.46 mL | 7.30 mL | 14.60 mL |
| 10 mM | 0.29 mL | 1.46 mL | 2.92 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Hoffmann SARS-CoV-2 cell entry depends on ACE2 and TMPRSS2 and is blocked by a clinically-proven protease inhibitor. Cell 2020 PMID: 32142651
- Wilcox and Mason Inhibition of cysteine proteinases in lysosomes and whole cells. Biochem.J. 1992 PMID: 1637341
- Sato Autophagy is activated in colorectal cancer cells and contributes to the tolerance to nutrient deprivation. Cancer Res. 2007 PMID: 17942897
- Tanada Lysosomal turnover, but not a cellular level, of endogenous LC3 is a marker for autophagy. Autophagy 2005 PMID: 16874052
- Kim Autophagy upregulation by inhibitors of caspase-3 and mTOR enhances radiotherapy in a mouse model of lung cancer. Autophagy 2008 PMID: 18424912
Product Specific Notices for E 64d
For research use only
Loading...
Loading...