5-Ethynyl-2'-deoxyuridine
Tocris Bioscience, part of Bio-Techne | Catalog # 7207
Thymidine analog for labeling DNA; can be linked to a fluorophore by click chemistry
Key Product Details
Description
Thymidine analog for labeling DNA; can be linked to a fluorophore by click chemistry
Product Description
5-Ethynyl-2'-deoxyuridine is a thymidine analog for labeling DNA. Can be linked to an azide fluorophore, such as 3-Azido-7-hydroxycoumarin (Cat. No. 7664) through click chemistry and used to detect DNA synthesis in proliferating cells in vivo and in vitro. Detects very low levels of cell proliferation in tissues with low cellular turnover. 5-Ethynyl-2'-deoxyuridine suppresses cell proliferation in osteosarcoma and glioblastoma cell lines, induces a 'futile' DNA repair cycle and induces DNA strand breaks and apoptosis at a higher rate than Temozolomide (Cat. No. 2706).Product Specifications
Molecular Weight
252.23
Formula
C11H12N2O5
Storage
Store at -20°C
Purity
≥95% (HPLC)
Chemical Name
5-ethynyl-1-((2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)pyrimidine-2,4(1H,3H)-dione
CAS Number
61135-33-9
PubChem ID
472172
InChI Key
CDEURGJCGCHYFH-DJLDLDEBSA-N
SMILES
O=C1N([C@@]2(O[C@H](CO)[C@@H](O)C2)[H])C=C(C#C)C(=O)N1
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 25.22 | 100 |
Preparing Stock Solutions
for 5-Ethynyl-2'-deoxyuridine
The following data is based on the product molecular weight 252.23
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 3.96 mL | 19.82 mL | 39.65 mL |
| 5 mM | 0.79 mL | 3.96 mL | 7.93 mL |
| 10 mM | 0.40 mL | 1.98 mL | 3.96 mL |
| 50 mM | 0.08 mL | 0.40 mL | 0.79 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Wang Nucleotide excision repair removes thymidine analog 5-ethynyl-2'-deoxyuridine from the mammalian genome. Proc.Natl.Acad.Sci.U.S.A. 2022 PMID: 35994676
- Salic & Mitchison A chemical method for fast and sensitive detection of DNA synthesis in vivo. Proc.Natl.Acad.Sci.U.S.A. 2008 PMID: 18272492
Product Specific Notices for 5-Ethynyl-2'-deoxyuridine
For research use only
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