3-Azido-7-hydroxycoumarin

Name: 3-Azido-7-hydroxycoumarin
Brand: Tocris Bioscience
SKU: 7664

Tocris Bioscience, part of Bio-Techne | Catalog # 7664

COA / SDS

Click-reactive fluorescent dye. Suitable for live cell imaging

Key Product Details

Description:	Click-reactive fluorescent dye. Suitable for live cell imaging
Chemical Name:	3-Azido-7-hydroxy-2H-1-benzopyran-2-one
Purity:	≥95% (HPLC)
Molecular Weight:	203.15

View Full Technical Details for 3-Azido-7-hydroxycoumarin

Technical Data
Citations

Biological Activity for 3-Azido-7-hydroxycoumarin

Key information: 3-Azido-7-hydroxycoumarin is a water-soluble fluorogenic fluorescent dye. Supplied with an azide reactive handle for click chemistry. It emits intense fluorescence only after 'click' reaction when it forms triazoles at the site of alkyne modified biomolecules. Suitable for live-cell imaging.

Application: Fluorescence microscopy. Can be used to label DNA in proliferating cells following 5-Ethynyl-2'-deoxyuridine (Cat.No. 7207) incorporation. Cell permeable.

Properties and Photophysical Data: Excitation and emission maxima (λ) are 404 nm and 477 nm, respectively, after click reaction.

Optical Data for 3-Azido-7-hydroxycoumarin

Emission Color	Blue
λ_abs	404 nm
λ_em	477 nm
Application	Fluorescence Microscopy

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Technical Data for 3-Azido-7-hydroxycoumarin

M. Wt	203.15
Formula	C₉H₅N₃O₃
Storage	Store at -20°C
Purity	≥95% (HPLC)
CAS Number	817638-68-9
PubChem ID	25127608
InChi Key	KKJNQKVCZCNJDI-UHFFFAOYSA-N
Smiles	[N-]=[N+]=NC1=CC=2C=CC(O)=CC2OC1=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for 3-Azido-7-hydroxycoumarin

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	20.32	100
	DMF	20.32	100

Preparing Stock Solutions for 3-Azido-7-hydroxycoumarin

The following data is based on the product molecular weight 203.15.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	4.92 mL	24.61 mL	49.22 mL
5 mM	0.98 mL	4.92 mL	9.84 mL
10 mM	0.49 mL	2.46 mL	4.92 mL
50 mM	0.10 mL	0.49 mL	0.98 mL

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References for 3-Azido-7-hydroxycoumarin

References are publications that support the biological activity of the product.