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I want to spend less hands-on time doing the immunoassayYou're in a race to make that next discovery, and Ella can help you get there faster. There's no need to split your time between running assays in the lab and analyzing answers at your desk. Simply add your sample and buffers to the Simple Plex cartridge, put it in Ella and start your run. There's only 10-15 minutes of hands-on assay prep time, and she gives you fully analyzed answers in an hour! |
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I need to transfer my immunoassay and get the same resultsWhen it's time to develop and transfer your immunoassay to other labs, sites or a contract research organization, Ella makes it easy. Because Simple Plex assays are precisely controlled in the cartridge, there's no chance for pipetting errors. You'll get the same reproducible answers across multiple users and multiple sites. Simply put, you'll get consistent answers anywhere, by anyone on any Ella. |
Citations are publications that use Bio-Techne products. Selected citations for Ella Automated Immunoassay System include:
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"We needed assays that were not only excellent at conserving our limited sample volumes but would allow us to test a lot of markers quickly and efficiently. The Luminex and Simple Plex assays proved to be the ideal choice in this case, giving us a level of accuracy and reproducibility that we’ve been unable to get with other platforms."
Dr. Melissa Munroe, M.D., Ph.D., Research Associate, Oklahoma Medical Research Foundation (OMRF)
"Simple Plex assays on Ella gave us speed and efficiency so we could complete our assays quickly, and the high quality of the data meant that we could easily see differences between disease state samples and controls. Plus, sample preparation was so simple it reduced chances of errors significantly."
Paulomi Aldo, Research Associate and Reproductive Sciences Core Manager, Department of Obstetrics, Gynecology and Reproductive Sciences, Yale University
"Ella allows for reproducible sample data with minimal sample input. The lower volume usage allows for the expansion of research into novel areas of angiogenesis and inflammation as they pertain to many forms of cancer."
Andrew Nixon Ph.D., M.B.A.,, Associate Professor, Medicine & Director, Duke University
"Ella is the closest I’ve seen to my goal of one day having a Star Trek-like tricorder. I can take just 25 μL of serum, add it to a cartridge, and in 90 minutes have immune profiling data."
E. Scott Halstead, M.D., Ph.D., Associate Professor, Department of Pediatrics, Penn State University College of Medicine
"Ella is a simple and fast immunoassay platform that would be ideal for implementation in higher biosafety facilities, like BSL-3, to study the acute phase of SARS-CoV-2 infection."
Anthony Tanoto Tan, Ph.D., Senior Research Fellow, Duke-NUS Medical School, Singapore
How can I locate the Certificate of Analysis (CoA) for a Simple Plex cartridge?
Proceed to the following link on ProteinSimple's website:https://www.proteinsimple.com/certificates.htmlFrom here, please enter the 6-digit part number and the lot number in the fields shown. (The part number is the last 6 digits of the product code, e.g., 000126 is the part number in SPCKA-PS-000126.)
Can expired cartridges be run on the Ella?
The Ella instrument is not programmed to reject expired cartridges, however we can only support the performance of cartridges within the expiration date.
What is the minimum sample volume required for Simple Plex assays?
A minimum of 50 µL of volume is required, regardless of dilution factor.
What is the expiration date of a Simple Plex cartridge?
Simple Plex cartridges expire one year from the date of manufacture.
What is the expiration date of Simple Plex controls?
Lyophilized controls are stable for up to 12 months from the date of receipt when stored at 2-8 °C. Reconstituted controls are stable for up to 6 months from date of preparation when stored at ≤ -70 °C. Storage at -20 °C is not recommended.
Ella does immunoassays in a microfluidic Simple Plex cartridge. It's like using a pre-kitted immunoassay, except everything's pre-loaded on the cartridge—even the calibration curve! All you need to do is add your sample and buffers and put the cartridge in Ella.
The immunoassay part works like this: sample runs through a microfluidic channel that binds your protein of interest. Next, Ella washes off unbound analyte and adds a detection reagent. Because each channel has three Glass Nano Reactors (GNRs) coated with a capture antibody, you get triplicate answers for each sample. Answers are then generated from the factory-calibrated standard curve that comes with every cartridge. Sound familiar? It's the same immunoassay you're used to, just without the errors that come with traditional assays.
Quantify cytokine and pro-inflammatory responses to SARS-CoV-2 infection in 90 minutes using an automated, multianalyte format.
Simple Plex assays enable rapid and reproducible quantification of inflammatory responses such as cytokine release syndrome and sepsis.
Simple Plex assays for cancer research have the sensitivity to profile low abundance cancer biomarkers.
Measure key neuroscience biomarkers such as neurofilament light (NF-L) and neurofilament heavy (NF-H) in CSF, plasma, and serum.
The Simple Plex HEK 293 HCP 3G assay on the Ella platform offers broad detection coverage of this host cell protein (HCP).
Iterate your workflow faster with Simple Plex CHO HCP 3G-1 assays for process-related impurity testing.
With a four-log range of detection, Simple Plex assays have the sensitivity to pick up even very low picogram-per-milliliter levels of endogenous mouse or rat analytes.
Get highly reproducible adeno-associated virus and lentivirus titer data with no manual steps in just 90 minutes.
| Description | Single Plex Specification | Multianalyte Specification | Multiplex Specification | Customizable Specification |
|---|---|---|---|---|
| Sample Volume Required | 2.5-25 µL | 2.5-25 µL | 2.5-25 µL | 2.5-25 µL |
| Glass Nano Reactor CV | <4% | |||
| Validated Intra-assay CV | <10% | N/A | ||
| Validated Inter-assay CV | <12% | <15% | N/A | |
| Dynamic range | 3-4 logs | |||
| Sensitivity | <1pg/mL (assay dependent) | |||
| Assay Runtime | 75 Minutes | 80 Minutes | ||
| Samples per run | Up to 72 | Up to 32 | Up to 32 | Up to 48 |
| Plex level | 1 | Up to 4 | Up to 8 | 1 |
| Results per run | Up to 72 | Up to 128 | Up to 256 | Up to 48 |
| Replicates per result | Triplicate | Triplicate | Duplicate | Triplicate |
| Ella Weight | 16 kg (35.3 lbs) | |||
| Ella Dimensions (closed) | 37 cm W x 54 cm D x 26 cm H | |||
| Ella Power | 100-240 V(AC), 300 VA, 45/65 Hz, Class I Installation Category(Over Voltage) II | |||
| Ella Operating temperature | 15 to 30 °C (59° to 86°F) | |||
| Ella Operating humidity | 15-80% relative, non-condensing | |||
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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