Mouse IgG2A Antibody

Catalog # MAB0032 | R&D Systems, Inc. a Bio-Techne Brand
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MAB0032-100
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Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # 344701

Product Summary for Mouse IgG2A Antibody

Immunogen

Cocktail of mouse IgG1 and IgG2A

Specificity

The specificity of this antibody was confirmed by direct ELISA on mouse IgG2A monoclonal antibodies, and it does not cross-react with IgG1, IgG2B, IgG3, IgM, IgA, or IgE antibodies. By flow cytometry, this isotype-specific secondary antibody detects primary antibodies of the mouse IgG2A isotype, and does not detect primary antibodies of mouse IgG1, IgG2B, or IgG3 class.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Scientific Data Images for Mouse IgG2A Antibody

Detection of Mouse IgG2A Primary Antibody by Flow Cytometry

Detection of Mouse IgG2A Primary Antibody by Flow Cytometry

Human peripheral blood lymphocytes were stained with A) Mouse Anti-Human CD4 Monoclonal Antibody (Catalog # MAB3791, Mouse IgG2A, filled histogram) or isotype control (Catalog # MAB003, open histogram), or B) Mouse Anti-Human CD8 alpha Monoclonal Antibody(Catalog # MAB1509, Mouse IgG2B, filled histogram) or isotype control (Catalog # MAB0041, open histogram), followed by Rat Anti-Mouse IgG2A isotype-specific secondary antibody (Catalog # MAB0032) and then Allophycocyanin-conjugated Anti-Rat IgG SecondaryAntibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse IgG2A Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: Mouse IgG2A Primary Antibody
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IgG2A

IgG is the most abundant of the five classes of mammalian immunoglobulins in serum. IgG molecules (~150 kDa) consist of two ~50 kDa heavy chains and two 25 kDa light chains connected by disulfide bonds. Antibody specificity is conferred by a combination of somatic rearrangement and hypermutation within variable regions. Mouse IgG isotypes IgG1, IgG2a and IgG2b share approximately 64-78% amino acid sequence identity within the heavy chain constant region domains (CH1, CH2 and CH3) and may vary in their interaction with complement, NK and mast cells, induction or inhibition by specific cytokines, and ability to cross the placenta.

Long Name

Immunoglobulin G2A Fc

Alternate Names

IgG2a

Product Documents for Mouse IgG2A Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse IgG2A Antibody

For research use only

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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