Rabbit anti-Monkey IgA Fc Secondary Antibody [FITC]

Monkey

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin

FITC (Excitation = 495 nm, Emission = 519 nm)

Polyclonal Rabbit

LYOPH mg/ml

Pools of purified IgA isolated from Rhesus monkey serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Precipitation reactions have been observed with immunoglobulins in Serum of other old-world Monkeys, including Cercopithecus, Cynomolgus and Baboon. The conjugate may also react with other species as has been observed for Chimpanzee and man.

The reactivity of the antiserum is directed to the Fc subunit of the IgA molecule, which expresses strict (class) specificity. In immunoelectrophoresis and radial in immunodiffusion using various antiserum concentrations against monkey serum, a single precipitin line has been obtained which shows a reaction of identity with the precipitin lines obtained with the purified IgA used as immunogens. It does not react with IgG, IgG/Fab fragments and IgM or any non-Ig protein in monkey serum, as tested by immunoelectrophoresis and double radial immunodiffusion.

Polyclonal

Rabbit

The lyophilized conjugate is shipped at ambient temperature and may be stored at 4C; prolonged storage at or below -20C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.

Ion exchange chromatography

Reconstitute by by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20C.

Lyophilized from PBS (pH 7.2)

No Preservative

LYOPH mg/ml

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Store at 4C in the dark.