Chinese Hamster Ovary cell line, CHO-derived rat Siglec-2/CD22 protein
Rat Siglec-2/CD22 (Trp24-Gly690) Accession # NP_001100973.1
Mouse IgG2a (Glu98-Lys330)
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
112-138 kDa, under reducing conditions
Measured by the ability of the immobilized protein to support the adhesion of human red blood cells. The ED50 for this effect is 0.15-1.8 μg/mL.
Recombinant Rat Siglec-2/CD22 Fc Chimera Protein, CF Scientific Data Examples
Recombinant Rat Siglec-2/CD22 Fc Chimera Protein Bioactivity.
Recombinant Rat Siglec-2/CD22 Fc Chimera (Catalog # 10572-SL) supports the adhesion of human red blood cells. The ED50 for this effect is 0.15-1.8 μg/mL.
Recombinant Rat Siglec-2/CD22 Fc Chimera Protein SDS-PAGE.
2 μg/lane of Recombinant Rat Siglec-2/CD22 Fc Chimera Protein (Catalog # 10572-SL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 112-138 kDa and 220-280 kDa, respectively.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitute at 500 μg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Sialic acid-binding immunoglobulin-like lectin 2 (Siglec-2), also known as B-cell receptor CD22 or B-lymphocyte cell adhesion molecule (BL-CAM), is a I-type (Ig-type) lectin belonging to the sialoadhesin subclass of the immunoglobulin superfamily (1). Fourteen human and nine mouse Siglecs have been characterized and are divided into 2 families: CD33 related and evolutionarily conserved (2, 3). The extracellular domain (ECD) of Siglecs are characterized by an N-terminal Ig-like V-type domain, which mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains (1 -3). The predominant form of human Siglec-2 contains a N-terminal Ig-like V-type domain, six Ig-like C2-type domains, a transmembrane region and a cytoplasmic tail with six tyrosine residues and four immunoreceptor tyrosine-based inhibition motifs (ITIMs) (1-3). A variant form of Siglec-2 missing two Ig-like C2-type domains along with a truncated cytoplasmic tail has also been identified (4). The mature ECD of rat Siglec-2 shares 58% and 76% amino acid sequence identity with human and mouse Siglec-2, respectively. Siglec-2 is an adhesion molecule that preferentially binds alpha 2,6- linked sialic acid on the same (cis) or adjacent (trans) cells (5). Besides its role as an adhesion molecule, Siglec-2 is a coreceptor that physically interacts with B-cell receptor (BCR), negatively regulating BCR signals by recruiting tyrosine phosphatase SHP-1 to its ITIMs. Phosphorylated Siglec-2 can also interact with other intracellular effector proteins such as Syk, PLC gamma, PI3 kinase and Grb-2, suggesting it may play a role in positive signaling (2). Another function of Siglec-2 is that it mediates the anti-phagocytic effect of alpha 2,6-linked sialic acid, and inhibition of Siglec-2 promotes the clearance of myelin debris, amyloid-beta oligomers and alpha -synuclein fibrils in vivo (6). Siglec-2 also plays a role in autoimmunity and has great potential for Siglec-2-based immunotherapeutics for the treatment of autoimmune diseases such as systemic lupus erythematosus (SLE) (7).
Sato, S. et al. (1996) Immunity. 5:551.
Crocker, P.R. and A. Varki (2001) Trends Immunol. 22:337.
Macauley, M.S. et al. (2014) Nature Rev Imm. 14:653.
Stamenkovic, I. and B. Seed (1990) Nature 345:74.
Collins, B.E. et al. (2004) Proc. Natl. Acad. Sci. 101:6104.
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