Recombinant Rat GITR Ligand/TNFSF18 Protein, CF

Name: Recombinant Rat GITR Ligand/TNFSF18 Protein, CF
Brand: R&D Systems
SKU: 9735-GL

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 9735-GL

Citations (1)

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Key Product Details

Source:	NS0
Accession #:	XP_008767927
Applications:	Bioactivity

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Product Specifications for Recombinant Rat GITR Ligand/TNFSF18 Protein, CF

Source	Mouse myeloma cell line, NS0-derived rat GITR Ligand/TNFSF18 protein Thr74-Ser200, with a N-terminal HA-tag
Purity	>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Tyr
Predicted Molecular Mass	16 kDa
SDS-PAGE	19-22 kDa, reducing conditions
Activity	Measured by its ability to induce IL-8 secretion in HT1080 human fibrosarcoma cells transfected with rat GITR. The ED₅₀ for this effect is 0.06-0.6 μg/mL in the presence of Mouse Anti-Hemagglutinin/HA Peptide Monoclonal Antibody (Catalog # MAB060).

Scientific Data Examples for Recombinant Rat GITR Ligand/TNFSF18 Protein, CF

	Recombinant Rat GITR Ligand/TNFSF18 Protein Bioactivity Recombinant Rat GITR Ligand/TNFSF18 (Catalog # 9735-GL) induces IL-8 secretion in HT1080 human fibrosarcoma cells transfected with rat GITR. The ED₅₀ for this effect is 0.06-0.6 µg/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9735-GL

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	12 months from date of receipt, ≤ -20 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

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Background: GITR Ligand/TNFSF18

GITR Ligand, also known as TNFSF18 and TL6, is an approximately 30 kDa type II transmembrane glycoprotein in the TNF superfamily (1). Rat GITR Ligand consists of a 20 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 132 aa extracellular domain (ECD) (2-4). Within the ECD, rat GITR Ligand shares 60.6% and 81.1% aa sequence identity with human and mouse GITR Ligand, respectively. GITR Ligand is expressed on antigen presenting cells, CD4^-CD8^- double negative thymic precursors, vascular endothelial cells, neurons, and in the eye (4-11). Its expression is transiently up-regulated by proinflammatory stimulation (5, 8, 11). The binding of GITR Ligand to GITR on mouse CD25⁺ T reg cells permits the reactivation of T cells from T_reg-induced suppression, although this does not appear to occur in humans (9, 12-14). GITR Ligand binding to GITR additionally provides a co-stimulatory signal to activated CD4⁺ and CD8⁺ T cells and NK cells (6, 15, 16). This interaction also induces reverse signaling in GITR Ligand expressing dendritic cells to suppress cellular activation through the same pathway induced by the immunosuppressant dexamethasone (17). In the brain, GITR Ligand/GITR interactions enhance NGF-mediated neurite outgrowth from sympathetic neurons (10).

Item	Value
References	Azuma, M. (2010) Crit. Rev. Immunol. 30:547. Yu, K.Y. et al. (2003) Biochem. Biophys. Res. Commun. 310:433. Kim, J.D. et al. (2003) Genes Immun. 4:564. Tone, M. et al. (2003) Proc. Natl. Acad. Sci. 100:15059. Stephens, G.L. et al. (2004) J. Immunol. 173:5008. Hanabuchi, S. et al. (2006) Blood 107:3617. Kamimura, Y. et al. (2009) J. Immunol. 182:2708. Hwang, H. et al. (2010) J. Neurosci. Res. 88:2188. Tuyaerts, S. et al. (2007) J. Leukoc. Biol. 82:93. O'Keefe, G.W. et al. (2008) Nat. Neurosci. 11:135. Kim, B.J. et al. (2004) Invest. Ophthalmol. Vis. Sci. 45:3170. Shimizu, J. et al. (2002) Nat. Immunol. 3:135. Ji, H. et al. (2004) J. Immunol. 172:5823. McHugh, R.S. et al. (2002) Immunity 16:311. Kanamaru, F. et al. (2004) J. Immunol. 172:7306. Ronchetti, S. et al. (2004) Eur. J. Immunol. 34:613. Grohmann, U. et al. (2007) Nat. Med. 13:579.
Long Name	Glucocorticoid Induced TNF Receptor Family Related Gene Ligand
Entrez Gene IDs	8995 (Human); 240873 (Mouse); 364031 (Rat)
Alternate Names	AITRL, AITRLMGC138237, Activation-inducible TNF-related ligand, GITR Ligand, GITRL, GITRLglucocorticoid-induced TNFR-related protein ligand, Glucocorticoid-induced TNF-related ligand, TL6AITR ligand, TNFSF18, hGITRLGITR ligand, tumor necrosis factor (ligand) superfamily, member 18, tumor necrosis factor ligand superfamily member 18

Citations for Recombinant Rat GITR Ligand/TNFSF18 Protein, CF (1)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Rat GITR Ligand/TNFSF18 Protein, CF include:

Species: Rat
Sample Types: Whole Cells
Applications: Cell Culture

Y He et al. (2022), GITR/GITRL reverse signalling modulates the proliferation of hepatic progenitor cells by recruiting ANXA2 to phosphorylate ERK1/2 and Akt Cell Death Disease, 2022;13(4):297.
PMID: 35379781

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