Recombinant Mouse TrkB Fc Chimera Protein, CF

Name: Recombinant Mouse TrkB Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 10266-TB
Price: 394 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 10266-TB

Citations (2)

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Key Product Details

Source:	NS0
Accession #:	P15209
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

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Product Specifications for Recombinant Mouse TrkB Fc Chimera Protein, CF

Source

Mouse myeloma cell line, NS0-derived mouse TrkB protein

Mouse TrkB (Cys32-His429) Accession # P15209	IEGRMDP	Mouse IgG_2a (Glu98-Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Cys32

Predicted Molecular Mass

72 kDa

SDS-PAGE

90-120 kDa, under reducing conditions

Activity

Measured by its ability to inhibit BDNF-induced proliferation of BaF‑TrkB‑BD mouse pro‑B cells transfected with TrkB. The ED₅₀ for this effect is 0.05-0.4 μg/mL.

Scientific Data Examples for Recombinant Mouse TrkB Fc Chimera Protein, CF

	Recombinant Mouse TrkB Fc Chimera Protein Bioactivity Recombinant Mouse TrkB Fc Chimera (Catalog # 10266-TB) inhibits BDNF-induced proliferation of BaF3 mouse pro-B cells transfected with human TrkB. The ED₅₀ for this effect is 0.05-0.4 µg/mL in the presence of 16 ng/mL of Recombinant Human BDNF (Catalog # 248-BDB).
	Recombinant Mouse TrkB Fc Chimera Protein SDS-PAGE 2 μg/lane of Recombinant Mouse TrkB Fc Chimera (Catalog # 10266-TB) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 90-120 kDa and 180-240 kDa, respectively.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10266-TB

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TrkB

Tropomyocin receptor kinase B (TrkB), also named Neurotrophic tyrosine kinase receptor type 3 (NTRK3) is a member of a nerve growth factor tyrosine kinase receptor family. There are three members of the Trk family, TrkA, TrkB and TrkC, and they bind a group of structurally related, secreted proteins termed neurotrophins, which play an important role in the development and function of the nervous system. The Trk family shares a conserved structural motif consisting of two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains in the extracellular region, a single transmembrane domain and an intracellular tyrosine kinase domain (3). Natural splice variants of the different Trks, lacking the first cysteine-rich domain, the first and second or all three of the leucine-rich motifs, or the tyrosine kinase domain, have been described (4). Mature mouse TrkB consists of a 398 amino acid (aa) extracellular domain (ECD) which shares 89% and 97% aa identity with human and rat TrkB, respectively. Each Trk family member exhibits different ligand specificities: TrkA binds NGF and NT-3, TrkB binds BDNF, NT-3 and NT-4/5, and TrkC only binds NT-3 (1, 2). The biological activities of the neurotrophins are mediated by binding to and activating two unrelated receptor types: the p75 neurotrophin receptor (p75NTR) and the Trk family of receptors (1, 2). P75NTR is a member of the tumor necrosis factor receptor superfamily (TNFRSF) and has been designated TNFRSF16. It binds all neurotrophins with low-affinity to transduce cellular signaling pathways that synergize or antagonize those activated by the Trk receptors. TrkB is primarily expressed in the nervous system (5). However, low levels of TrkB expression have also been observed in a wide variety of tissues (pancreas, kidneys, ovary) outside the nervous system (7). Trk receptor interactions with NGF play major roles in the development of the sympathetic nervous system and are essential for neuronal survival in vivo (8). Agonists of TrkB have shown enhanced endogenous analgesia by restoring stimuli-response activity in spinal nerve damaged rats (9) and inhibition of the Trk receptors may have a range of therapeutic implications (10).

Item	Value
References	Huang, E.J. and L.F. Reichardt. (2003) Annu. Rev. Biochem. 72:609. Dechant, G. (2001) Cell Tissue Res. 305:229. Schneider, R. and M. Schweiger (1991) Oncogene 6:1807 Ninkina, N. et al. (1997) J. Biol. Chem. 272:13019. Soppet, D. et al. (1991) Cell 65:895. Middlemas, D.S. et al. (1991) Mol. Cell Biol. 11:143. Shelton, D. L. et al. (1995) J. Neurosci. 15:447. Fagan, A. et al. (1996) J Neurosci. 16:6208. Yan, W. et al. (2019) J Med. Chem. 62:1731. Suto, T. et al. (2019) J Pain. 20:600.
Long Name	Neurotrophic Tyrosine Kinase Receptor B
Entrez Gene IDs	4915 (Human); 18212 (Mouse); 25054 (Rat)
Alternate Names	BDNF/NT-3 growth factors receptor, EC 2.7.10, EC 2.7.10.1, GP145-TrkB, NTRK2, Neurotrophic tyrosine kinase receptor type 2, TRKBBDNF-tropomyosine receptor kinase B, TrkB, TrkB tyrosine kinase, neurotrophic tyrosine kinase, receptor, type 2, trk-B, tyrosine kinase receptor B

Citations for Recombinant Mouse TrkB Fc Chimera Protein, CF (2)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse TrkB Fc Chimera Protein, CF include:

Species: Rat
Sample Types: In Vivo
Applications: In Vivo

X Ding et al. (2022), Sciatic nerve block downregulates the BDNF pathway to alleviate the neonatal incision-induced exaggeration of incisional pain via decreasing microglial activation Brain, Behavior, and Immunity, 105(0):204-224.
PMID: 35853558
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

C Frick et al. (2020), Age-Dependency of Neurite Outgrowth in Postnatal Mouse Cochlear Spiral Ganglion Explants Brain Sci, 10(9).
PMID: 32839381

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