Recombinant Mouse TREM2 His-tag Protein, CF

Name: Recombinant Mouse TREM2 His-tag Protein, CF
Brand: R&D Systems
SKU: 9228-T2

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 9228-T2

Citations (1)

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Key Product Details

Source:	NS0
Accession #:	Q99NH8
Applications:	Bioactivity

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Product Specifications for Recombinant Mouse TREM2 His-tag Protein, CF

Source	Mouse myeloma cell line, NS0-derived mouse TREM2 protein Leu19-Ser171, with a C-terminal 6-His tag
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Leu19
Predicted Molecular Mass	18 kDa
SDS-PAGE	25 - 75 kDa, under reducing conditions.
Activity	Measured by its ability to bind fluorescein-conjugated S. aureus Bioparticles. Daws, M.R. et al. (2003) J. Immunol. 171:594. The ED₅₀ for this effect is 20-120 ng/mL using a His tag antibody coated plate.

Scientific Data Examples for Recombinant Mouse TREM2 His-tag Protein, CF

	Recombinant Mouse TREM2 His-tag Protein Bioactivity Recombinant Mouse TREM-2 (Catalog # 9228-T2) binds fluorescein-conjugatedS. aureusBioparticles. The ED₅₀ for this effect is 20-120 ng/mL using an anti-His tag antibody (Catalog # MAB050) coated plate.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9228-T2

Formulation	Lyophilized from a 0.2 μm filtered solution in HEPES and NaCl.
Reconstitution	Reconstitute at 500 μg/mL in water. Reconstitution Buffer Available: Size / Price Qty Loading... RB07:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TREM2

TREM-2 (Triggering Receptor Expressed on Myeloid cells-2) is a 35 kDa type I transmembrane member of the TREM family and Ig superfamily (1). Mature mouse TREM-2 consists of a 153 amino acid (aa) extracellular domain (ECD) with one Ig-like domain, a 21 aa transmembrane segment, and a 35 aa cytoplasmic domain (2). Within the ECD, mouse TREM-2 shares 73% and 90% aa sequence identity with human and rat TREM-2, respectively. Soluble forms of the TREM-2 ECD are generated by alternative splicing or proteolytic cleavage, and the cytoplasmic domain can be liberated by gamma-Secretase mediated intramembrane cleavage (3). A positively charged lysine within the transmembrane segment allows association with the signal adapter protein, DAP12 and inhibition of macrophage activation (4, 5). TREM-2 is expressed on macrophages, immature myeloid dendritic cells, osteoclasts, microglia, and adipocytes (5-9). It promotes the differentiation and function of osteoclasts, the production of inflammatory cytokines by adipocytes, insulin resistance, and the phagocytic clearance of bacteria (9-11). In the CNS, TREM-2 binds to ApoE, ApoA1, and ApoB and mediates the clearance of apoptotic neurons, amyloid plaques, and cell debris following demyelination (6-8, 12). TREM-2 also interacts with and modifies signaling through Plexin A1 on dendritic cells and osteoclasts (13). Mutations in TREM-2 or DAP12 are associated with the development of Alzheimer's disease and Nasu-Hakola disease (NHD/PLOSL) which is characterized by presenile dementia and bone cysts (14, 15). Soluble TREM-2 is elevated in cerebrospinal fluid of patients with active multiple sclerosis (MS), and TREM-2 blockade exacerbates disease symptoms in the experimental EAE model of MS (16, 17).

Item	Value
References	Painter, M.M. et al. (2015) Mol. Neurodegener. 10:43. Daws, M.R. et al. (2001) Eur. J. Immunol. 31:783. Wunderlich, P. et al. (2013) J. Biol. Chem. 288:33027. Hamerman, J. A. et al. (2006) J. Immunol. 177:2051. Turnbull, I.R. et al. (2006) J. Immunol. 177:3520. Takahashi, K. et al. (2005) J. Exp. Med. 201:647. Atagi, Y. et al. (2015) J. Biol. Chem. 290:26043. Wang, Y. et al. (2016) J. Exp. Med. 213:667. Cella, M. et al. (2003) J. Exp. Med. 198:645. Park, M. et al. (2015) Diabetes 64:117. N'Diaye, E-N. et al. (2009) J. Cell Biol. 184:215. Poliani, P.L. et al. (2015) J. Clin. Invest. 125:2161. Takegahara, N. et al. (2006) Nat. Cell Biol. 8:615. Colonna, M. and Y. Wang (2016) Nat. Rev. Neurosci. 17:201. Paloneva, J. et al. (2002) Am. J. Hum. Genet. 71:656. Piccio, L. et al. (2008) Brain 131:3081. Piccio, L. et al. (2007) Eur. J. Immunol. 37:1290.
Long Name	Triggering Receptor Expressed on Myeloid Cells 2
Entrez Gene IDs	54209 (Human); 102133279 (Cynomolgus Monkey)
Alternate Names	PLOSL2, TREM-2, TREM-2triggering receptor expressed on myeloid cells 2a, TREM2, Trem2a, Trem2b, Trem2c, Triggering receptor expressed on monocytes 2, triggering receptor expressed on myeloid cells 2

Citations for Recombinant Mouse TREM2 His-tag Protein, CF (1)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse TREM2 His-tag Protein, CF include:

Species: Mouse
Sample Types: Cell Lysates
Applications: ELISA Standard

VS Jadhav et al. (2020), Trem2 Y38C mutation and loss of Trem2 impairs neuronal synapses in adult mice Mol Neurodegener, 15(1):62.
PMID: 33115519

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