Recombinant Mouse TREM2 Fc Chimera Protein, CF

Name: Recombinant Mouse TREM2 Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 1729-T2
Price: 394 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 1729-T2

Citations (6)

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Key Product Details

Source:	NS0
Accession #:	Q99NH8
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Mouse TREM2 Fc Chimera Protein, CF

Product Specifications for Recombinant Mouse TREM2 Fc Chimera Protein, CF

Source

Mouse myeloma cell line, NS0-derived mouse TREM-2 protein

Mouse TREM-2 (Leu19-Pro168) Accession # Q99NH8	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Leu19

Predicted Molecular Mass

43 kDa (monomer)

SDS-PAGE

60-70 kDa, reducing conditions

Activity

Measured by its ability to bind fluorescein-conjugated S. aureus Bioparticles. Daws, M.R. et al. (2003) J. Immunol. 171:594. The ED₅₀ for this effect is 0.3-1.5 µg/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1729-T2

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TREM2

TREM-2 (Triggering Receptor Expressed on Myeloid cells-2) is a type I transmembrane member of the TREM family and Ig superfamily (1, 2). Mouse TREM-2 cDNA encodes 227 amino acids (aa) that include an 18 aa signal sequence, a 153 aa extracellular domain (ECD) with one V-type Ig-like domain, a 21 aa transmembrane (TM) domain, and a 35 aa cytoplasmic tail (1). A soluble, 249 aa form of TREM-2 (TREM-2b) created by alternate splicing diverges at aa 161 (1-3). Within aa 19-161, mouse TREM-2b shares 73%, 88%, 73% and 71% aa sequence identity with the human, rat, bovine and equine TREM-2 ECD, respectively. In both TREM-1 and TREM-2, a positively charged lysine within the TM domain allows association and signaling through the signal adapter protein, DAP12 (1, 2). While TREM-1 induces macrophage activation, TREM-2 coupled with the linker protein LAT2 is inhibitory (5-7). TREM-2 is detected on newly differentiated and alternatively activated tissue macrophages, immature myeloid dendritic cells and osteoclasts, but not on circulating or tissue-resident myeloid cells (2, 6, 7). It is active in several settings that involve membrane fusion, including phagocytosis of microbes by macrophages and apoptotic neurons by microglia, and formation of osteoclasts and multinucleated giant cells from macrophages (2, 4, 8-11). TREM-2 is also interacts with and modifies signaling through dendritic cell and osteoclast Plexin A1, a semaphorin receptor (12). Mutations in human TREM-2 or DAP12 can result in Nasu-Hakola disease (NHD), also called PLOSL (polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy; 2, 11, 13). NHD patients show presenile dementia and bone cysts, presumably due to persistence of apoptotic neurons and faulty osteoclast development (2, 11, 13). Soluble TREM-2 antagonizes full-length TREM-2 and is elevated in cerebrospinal fluid of patients with active multiple sclerosis, and blockade of full-length TREM-2 exacerbates the mouse multiple sclerosis model, EAE (2, 4, 14).

Item	Value
References	Daws, M. et al. (2001) Eur. J. Immunol. 31:783. Ford, J.W. and D.W. McVicar (2009) Curr. Opin. Immunol. 21:38. Swiss-Prot Accession Q99NH8 Piccio, L. et al. (2008) Brain 131:3081. Hamerman, J. A. et al. (2006) J. Immunol. 177:2051. Whittaker, G.C. et al. (2010) J. Biol. Chem. 285:2976. Turnbull, I.R. et al. (2006) J. Immunol. 177:3520. N’Diaye, E-N. et al. (2009) J. Cell Biol. 184:215. Helming, L. et al. (2008) Sci. Signal. 1:ra11. Takahashi, K. et al. (2005) J. Exp. Med. 201:647. Cella, M. et al. (2003) J. Exp. Med. 198:645. Takegahara, N. et al. (2006) Nat. Cell Biol. 8:615. Paloneva, J. et al. (2002) Am. J. Hum. Genet. 71:656. Piccio, L. et al. (2007) Eur. J. Immunol. 37:1290.
Long Name	Triggering Receptor Expressed on Myeloid Cells 2
Entrez Gene IDs	54209 (Human); 102133279 (Cynomolgus Monkey)
Alternate Names	PLOSL2, TREM-2, TREM-2triggering receptor expressed on myeloid cells 2a, TREM2, Trem2a, Trem2b, Trem2c, Triggering receptor expressed on monocytes 2, triggering receptor expressed on myeloid cells 2

Citations for Recombinant Mouse TREM2 Fc Chimera Protein, CF (6)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse TREM2 Fc Chimera Protein, CF include:

Species: Mouse
Sample Types: Cell Culture Supernates
Applications: Reference Standard

SF You et al. (2023-01-01), MS4A4A modifies the risk of Alzheimer disease by regulating lipid metabolism and immune response in a unique microglia state medRxiv : the preprint server for health sciences, 0(0).
PMID: 36798226
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Y Dong et al. (2021-01-01), Oxidized phosphatidylcholines found in multiple sclerosis lesions mediate neurodegeneration and are neutralized by microglia Nature Neuroscience, 0(0).
PMID: 33603230
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay

Kawabori M et al. (2015-01-01), Triggering receptor expressed on myeloid cells 2 (TREM2) deficiency attenuates phagocytic activities of microglia and exacerbates ischemic damage in experimental stroke. J Neurosci, 35(8):3384-96.
PMID: 25716838
Species: N/A
Sample Types: Antibody
Applications: Neutralization

Chertoff M et al. (2013-01-01), Differential modulation of TREM2 protein during postnatal brain development in mice. PLoS ONE, 8(8):e72083.
PMID: 23977213
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

Hsieh CL et al. (2009-01-01), A role for TREM2 ligands in the phagocytosis of apoptotic neuronal cells by microglia. J. Neurochem., 109(4):1144-56.
PMID: 19302484
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

Hamerman JA et al. (2006-01-01), Cutting edge: inhibition of TLR and FcR responses in macrophages by triggering receptor expressed on myeloid cells (TREM)-2 and DAP12. J. Immunol., 177(4):2051-5.
PMID: 16887962

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Showing 1 - 6 of 6 citations Showing All

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Recombinant Mouse TREM2 Fc Chimera Protein, CF

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