This protein datasheet indicates I need to use a cross-linking antibody, Catalog # MAB050, for biological activity. What is this antibody and is it really necessary?

Name: Recombinant Mouse TRAIL R2/TNFRSF10B Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 721-DR

The antibody is directed against a 6x histidine repeat and is recommended for use as a cross-linker of proteins with 6x his-tag. Crosslinking is often used for proteins that require receptor trimerization and can result greater biological activity. R&D Systems Quality Control tests the performance of these proteins in the presence of the cross-linking antibody. Therefore, it is necessary to use this antibody when trying to achieve the same level of specific activity described in the datasheet.

Recombinant Mouse TRAIL R2/TNFRSF10B Fc Chimera Protein, CF

Catalog # 721-DR | R&D Systems, Inc. a Bio-Techne Brand

Citations (2)

Product Datasheet / COA / SDS

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Key Product Details

Source:	Sf 21 (baculovirus)
Accession #:	Q6GSD9
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Mouse TRAIL R2/TNFRSF10B Fc Chimera Protein, CF

Technical Data
Citations (2) / Reviews / FAQs

Product Specifications for Recombinant Mouse TRAIL R2/TNFRSF10B Fc Chimera Protein, CF

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse TRAIL R2/TNFRSF10B protein

Mouse TRAIL R2 (Asn53 - Ser177) & (Arg58 - Ser177) Accession # Q6GSD9	IEGRMD	Human IgG₁ (Pro100 - Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Asn53 & Arg58

Predicted Molecular Mass

40 kDa (monomer)

SDS-PAGE

40 kDa (monomer)

Activity

Measured by its ability to inhibit TRAIL-mediated cytotoxicity using L‑929 mouse fibroblast cells treated with TRAIL. The ED₅₀ for this effect is 3‑12 ng/mL in the presence of 20 ng/mL of rhTRAIL or 12 ng/mL cross-linked rhTRAIL (Catalog # 375-TL and MAB050).

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

721-DR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: TRAIL R2/TNFRSF10B

Mouse TRAIL R2, also called DR5, TRICK 2, TNFRSF10B, and MK is a type 1 TNF R superfamily, membrane protein which is a receptor for TRAIL (APO2 ligand). Mouse TRAIL R2 cDNA encodes a 381 amino acid residue precursor protein containing an extracellular cysteine-rich domain, a transmembrane domain and a cytoplasmic death domain. Human and mouse TRAIL R2 share 49% amino acid sequence similarity. The death domains of human TRAIL R1 and TRAIL R2 share high homology with the death domain of mouse TRAIL R2, 76% and 79%, respectively. Binding of trimeric TRAIL to TRAIL R2 induces apoptosis. The induction of apoptosis likely requires oligomerization of the receptor. The mouse TRAIL R2/Fc chimera neutralizes the ability of rhTRAIL to induce apoptosis. Besides the death domain containing receptors TRAIL R2 and TRAIL R1/DR4, three TRAIL decoy receptors, TRAIL R3/DcR1, TRAIL R4/DcR2, and OPG, have been reported.

Item	Value
References	Chaudhary, P.M. et al. (1997) Immunity 7:821. Walczak, H. et al. (1997) EMBO J. 16:5386. Golstein, P. (1997) Curr. Biol. 7:R750. Wu, G.S. et al. (1999) Cancer Research 59:2770.
Long Name	TRAIL Receptor 2
Entrez Gene IDs	8795 (Human); 21933 (Mouse)
Alternate Names	CD262, CD262 antigen, DR5, DR5TRICK2B, Death receptor 5, Fas-like protein, KILLER/DR5, KILLERapoptosis inducing protein TRICK2A/2B, TNF-related apoptosis-inducing ligand receptor 2, TNFRSF10B, TRAIL R2, TRAIL receptor 2, TRAIL-R2apoptosis inducing receptor TRAIL-R2, TRAILR2, TRAILR2cytotoxic TRAIL receptor-2, TRICK2, TRICK2A, TRICKB, ZTNFR9, death domain containing receptor for TRAIL/Apo-2L, tumor necrosis factor receptor superfamily member 10B, tumor necrosis factor receptor superfamily, member 10b, tumor necrosis factor receptor-like protein ZTNFR9

Citations for Recombinant Mouse TRAIL R2/TNFRSF10B Fc Chimera Protein, CF (2)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse TRAIL R2/TNFRSF10B Fc Chimera Protein, CF include:

Species: N/A
Sample Types: Recombinant Protein
Applications: Bioassay

Luo et al. (2023-07-26), Heparan sulfate promotes TRAIL-induced tumor cell apoptosis bioRxiv : the preprint server for biology
PMID: 37546770
Species: Mouse
Sample Types: Recombinant Protein

JS Park et al. (2019-03-08), Targeting of dermal myofibroblasts through death receptor 5 arrests fibrosis in mouse models of scleroderma Nat Commun, 2019-010(1):1128.
PMID: 30850660

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FAQs for Recombinant Mouse TRAIL R2/TNFRSF10B Fc Chimera Protein, CF

This protein datasheet indicates I need to use a cross-linking antibody, Catalog # MAB050, for biological activity. What is this antibody and is it really necessary?

The antibody is directed against a 6x histidine repeat and is recommended for use as a cross-linker of proteins with 6x his-tag. Crosslinking is often used for proteins that require receptor trimerization and can result greater biological activity. R&D Systems Quality Control tests the performance of these proteins in the presence of the cross-linking antibody. Therefore, it is necessary to use this antibody when trying to achieve the same level of specific activity described in the datasheet.

Recombinant Mouse TRAIL R2/TNFRSF10B Fc Chimera Protein, CF

Catalog # 721-DR | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Product Specifications for Recombinant Mouse TRAIL R2/TNFRSF10B Fc Chimera Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: TRAIL R2/TNFRSF10B

Citations for Recombinant Mouse TRAIL R2/TNFRSF10B Fc Chimera Protein, CF (2)

FAQs for Recombinant Mouse TRAIL R2/TNFRSF10B Fc Chimera Protein, CF

Product Documents for Recombinant Mouse TRAIL R2/TNFRSF10B Fc Chimera Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

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