Recombinant Mouse TMEFF2/Tomoregulin-2 Fc Protein, CF

Catalog # 11204-TF | R&D Systems, Inc. a Bio-Techne Brand
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Key Product Details

Accession #

Q9QYM9.1

Source

NS0

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse TMEFF2/Tomoregulin-2 protein
Mouse TMEFF2
(Phe41-Val320)
Accession # Q9QYM9.1		 IEGRMDP Mouse IgG2A
(Glu98-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Phe41

Predicted Molecular Mass

58 kDa

SDS-PAGE

77-90 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse PDGF-AB  (Catalog # 10839-AB) is immobilized at 2 µg/mL (100 µL/well), Recombinant Mouse TMEFF2/Tomoregulin-2 Fc Chimera (Catalog # 11204-TF) binds with an ED50 of 1.00-9.00 µg/mL.

Scientific Data Images for Recombinant Mouse TMEFF2/Tomoregulin-2 Fc Protein, CF

Recombinant Mouse TMEFF2/Tomoregulin‑2 Fc Chimera Protein SDS-PAGE.

2 μg/lane of Recombinant Mouse TMEFF2/Tomoregulin‑2 Fc Chimera Protein (Catalog # 11204-TF) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 77-90 kDa and 150-180 kDa, respectively.

Formulation, Preparation and Storage

11204-TF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TMEFF2/Tomoregulin-2

The transmembrane protein with an EGF-like and two Follistatin-like domains 2 (TMEFF2) encodes a 374 residue long single polypeptide, type-I transmembrane proteoglycan. It is also known as HPP1, tomoregulin (TR), transmembrane protein TENB2, cancer/testis antigen family 120, member 2 (CT120.2), and transmembrane protein containing EGF and follistatin domains (TPEF). TMEFF2 amino acid sequences are 98.9% identical for human and mouse orthologs (1). The differential methylation of TMEFF2 can be related to the response to therapy and survival outcomes of the stages of cancers such as breast, prostate, lung, bladder, colon and rectal, gallbladder, renal, oesophageal, cardiac, stomach/gastric, ovarian, multiple myeloma, glioblastoma, and mesothelioma. The range of tissue expression of TMEFF2 functions as a possible biomarker and/or therapeutic target (1). Studies showed that TMEFF2 binds and inhibits PDGF-AA (2,3). It also inhibits the proliferation of DU145 and PC3 prostate cancer cell lines (4). The amyloid-b protein, its precursor AbPP, and amyloid- b proteins AbPOs also binds TMEFF2 suggesting a neuroprotective role on Alzheimer's disease (5). TMEFF2 diverse function includes metabolism, embryonic development, cytoskeletal binding, extracellular matrix binding, chromatin binding and interaction of RNA polymerase II with DNA (6), neuronal development (7).

References

  1. Masood, M. et al. (2020) Cancers 12:3862.
  2. Lin, K. et al. (2011) PLoS ONE 6:e18608.
  3. Huang, H. et al. (2017) Sci. Rep. 7:337.
  4. Gery, S. et al. (2002)Oncogene 21:4739.
  5. Hong, H.S. et al. (2015) Alzheimers Dis. 48:731.
  6. Gao, L. et al. (2020) Life Sci. 243:116910.
  7. Horie, M. et al. (2000) Genomics 67:146.

Long Name

Transmembrane Protein with EFG-like and two Follistatin-like Domains

Alternate Names

TENB2, Tomoregulin-2, Tomoregulin2

Entrez Gene IDs

23671 (Human); 56363 (Mouse)

Gene Symbol

TMEFF2

UniProt

Q9QYM9.1

Product Documents for Recombinant Mouse TMEFF2/Tomoregulin-2 Fc Protein, CF

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Product Specific Notices for Recombinant Mouse TMEFF2/Tomoregulin-2 Fc Protein, CF

For research use only

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