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Recombinant Mouse PDGF-AB Protein, CF

Bio-Techne includes R&D Systems | Catalog # 10839-AB

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10839-AB-010
10839-AB-050

Key Product Details

Source

E. coli

Structure / Form

Disulfide-linked heterodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

E. coli-derived mouse PDGF-AB protein
Mouse PDGF-A
(Ser87-Thr211)
Accession # P20033.2
Mouse PDGF-B
(Ser82-Thr190)
Accession # P31240.1
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser87 (A chain) & Ser82 (B chain)

Predicted Molecular Mass

14 kDa (A chain) & 12 kDa ( beta chain)

SDS-PAGE

17.5 kDa (A chain) & 12.5 kDa (B chain), under reducing conditions.

Activity

Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749.
The ED50 for this effect is 15.0-75.0 ng/mL.

Scientific Data Images for Recombinant Mouse PDGF-AB Protein, CF

Recombinant Mouse PDGF-AB Protein Bioactivity.

Recombinant Mouse PDGF-AB induces NR6R-3T3 mouse fibroblast cell proliferation. The ED50 for this effect is 15.0-75.0 ng/mL.

Recombinant Mouse PDGF-AB Protein SDS-PAGE.

2 µg/lane of Recombinant Mouse PDGF-AB (Catalog # 10839-AB) was resolved by SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 17.5 kDa (A chain) & 12.5 kDa (B chain) and 29 kDa (heterodimer), respectively.

Formulation, Preparation and Storage

10839-AB
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PDGF-AB

Platelet-Derived Growth Factors (PDGFs) are a family of four cystine-knot-type growth factors (PDGF-A, -B, -C and -D) which control the growth of connective tissue cells such as fibroblasts and smooth muscle cells (1, 2). They comprise of four homodimers (PDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD) and one heterodimer (PDGF-AB). All four PDGF genes contain a signal sequence, a propeptide and a mature form (2, 3). Mature mouse PDGFA is 95% and 98% identical to human and rat PDGFA respectively whereas mature mouse PDGFB is 89% and 98% homologous to human and rat PDGFB respectively. In general, PDGFs are expressed by a number of different cells and tissues including endothelial cells, epithelial cells, hematopoietic cells, connective tissue, nervous tissue, brain, muscle, kidney and liver. PDGF expression is highly regulated with PDGF levels increasing following injury and/or disease. An analysis of PDGF purified from human platelets suggest that approximately 70% of sera-derived PDGF is PDGF-AB (4). PDGF isoforms exert their cellular effect by binding to the structurally similar receptor tyrosine kinases PDGF receptor-alpha and PDGF receptor-beta. PDGF-AB binding induces receptor dimerization resulting in alpha alpha receptor homodimers and alpha beta receptor heterodimers. Binding of PDGF receptors has been reported to result in stimulation of mitogenicity and chemotaxis of fibroblasts, stimulation of granule release by neutrophils and monocytes, facilitation of steroid synthesis by Leydig cells, stimulation of neutrophil phagocytosis, stimulation of collagen, fibronectin, proteoglycan, and hyaluronic acid synthesis, modulation of thrombospondin expression and secretion, stimulation of collagenase activity and secretion, induction of contraction of rat aorta strips in vitro, and transient induction of T cell IL-2 secretion accompanied by a down-regulation of IL-4 and IFN-gamma production (5).

References

  1. Kohler, N. Lipton, A. (1974) Exp Cell Res. 87:297.
  2. Ross, R. et al. (1974) Proc Natl Acad Sci U.S.A. 71:1207.
  3. Frediksson, L. et al. (2004) Cytokine Growth Factor Rev. 15:197.
  4. Chen, P-H. et al. (2013) BiochimBiophysActa 1834:2176.
  5. Heldin, C-H. Eriksson, U. and Ostman, A. (2002) Arch. Biochem. Biophysic. 398:284.

Long Name

Platelet-derived Growth Factor AB

Alternate Names

PDGFAB

Additional PDGF-AB Products

Product Documents for Recombinant Mouse PDGF-AB Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse PDGF-AB Protein, CF

For research use only

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