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Recombinant Mouse PVRIG mFc Chimera Protein, CF

Bio-Techne includes R&D Systems | Catalog # 11315-PV

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11315-PV-050

Key Product Details

Source

HEK293

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived mouse PVRIG protein
Mouse PVRIG
(Ser35-Asp165)
Accession # NP_001365367.1
IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu33, Ser35

Predicted Molecular Mass

41 kDa (monomer)

SDS-PAGE

48-61 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse PVRIG mFc Chimera (Catalog # 11315-PV immobilized at 1 µg/mL (100 µL/well), Recombinant Mouse Nectin-2/CD112 (Catalog # 3869-N2) binds with an ED50 of 0.150-1.20 µg/mL.

Scientific Data Images for Recombinant Mouse PVRIG mFc Chimera Protein, CF

Recombinant Mouse PVRIG mFc Chimera Protein Binding Activity.

When Recombinant Mouse PVRIG mFc Chimera (Catalog # 11315-PV) is immobilized at 1 µg/mL (100 µL/well), Recombinant Mouse Nectin‑2/CD112 (3869-N2) binds with an ED50 of 0.150-1.20 µg/mL.

Recombinant Mouse PVRIG mFc Chimera Protein SDS-PAGE.

2 μg/lane of Recombinant Mouse PVRIG mFc Chimera Protein (Catalog # 11315-PV) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 48-61 kDa and 96-122 kDa, respectively.

Formulation, Preparation and Storage

11315-PV
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PVRIG

PVRIG (poliovirus receptor related immunoglobulin domain-containing protein), also known as CD112 receptor (CD112R), is an approximately 34 kDa single transmembrane protein in the poliovirus receptor-like protein (PVR) family (1). It is composed of a single extracellular IgV domain, one transmembrane domain, and a long intracellular domain. The intracellular domain contains two tyrosine residues, one within an ITIM-like motif that is a potential docking site for phosphatases (1). The extracellular domain sequence of mouse PVRIG have approximately 64% and 87% similarity with human and rat PVRIG, respectively. The human PVRIG gene is preferentially expressed in lymphocytes, such as T cells and NK cells, but not in monocyte derived dendritic cells (1). PVRIG functions as a cell surface receptor for Nectin-2/CD112, a cell surface protein that is widely expressed on antigen-presenting cells and tumor cells. Disrupting the PVRIG/Nectin-2 interaction enhances human T cell response, suggesting PVRIG is a novel checkpoint for human T cells (1-3). Blocking PVRIG with neutralizing antibody is a feasible approach for NK cell mediated immunotherapy of cancers (4, 5).

References

  1. Zhu, Y. et al. (2016) J. Exp. Med. 213:167.
  2. Whelan, S. et al. (2019) Cancer Immunol Res. 7:257.
  3. Murter, B. et al. (2019) Cancer Immunol Res. 7:244.
  4. Li, J. et al. (2020) Haematologica. DOI:10.3324/haematol.2020.258574. 
  5. Sanchez-Correa, B. et al. (2019) Cancers (Basel) 11:877.

Long Name

Poliovirus Receptor Related Immunoglobulin Domain Containing

Alternate Names

C7orf15, CD112R

Entrez Gene IDs

79037 (Human); 102640920 (Mouse); 102127364 (Cynomolgus Monkey)

Gene Symbol

PVRIG

UniProt

Additional PVRIG Products

Product Documents for Recombinant Mouse PVRIG mFc Chimera Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse PVRIG mFc Chimera Protein, CF

For research use only

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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