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Recombinant Mouse Nectin-2/CD112 Protein, CF

Bio-Techne includes R&D Systems | Catalog # 3869-N2

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3869-N2-050

Key Product Details

Source

NS0

Accession #

Structure / Form

Monomer

Conjugate

Unconjugated

Applications

Binding Activity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Nectin-2/CD112 protein
Gln32-Gly351, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained: Gln32 predicted

Predicted Molecular Mass

35.4 kDa

SDS-PAGE

45-50 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse Nectin-2/CD112 is coated at 1 μg/mL (100 μL/well), the concentration of Recombinant Mouse DNAM-1 Fc Chimera (Catalog # 4436-DN) that produces 50% optimal binding response is 0.3-1.8 μg/mL.

Formulation, Preparation and Storage

3869-N2
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nectin-2/CD112

Nectins are a small family of Ca++-independent immunoglobulin (Ig)-like cell adhesion molecules (CAMs) that control cell adhesion, proliferation, and migration (1, 2, 3). The name Nectin derives from the Latin word necto, which means “to connect”. The Nectin family contains four members (Nectin-1 - 4), all of which show alternate splicing, a transmembrane (TM) region (except for Nectin-1 gamma which is secreted), and three extracellular Ig-domains. Nectins are highly homologous to the human receptor for poliovirus, and as such have been given the alternate name of poliovirus receptor-related proteins. They do not, however, appear to bind poliovirus (1). Mouse Nectin-2 is a 70 to 78 kDa type I TM glycoprotein that is found on a variety of cell types (4, 5). It has two splice forms (4, 6, 7). Nectin-2 alpha/PRR2 is a 65 kDa short form and is synthesized as a 467 amino acid precursor. It contains a 31 aa signal sequence, a 315 aa extracellular domain (ECD), a 28 aa TM segment, and a 93 aa cytoplasmic region. The ECD contains one N-terminal V-type Ig domain and two 85 - 95 aa C2-type Ig-like domains (aa 153 - 337) (8). The V-domain is believed to mediate Nectin binding to its ligands (9). A long, 78 kDa, 530 aa isoform of mouse Nectin-2 (Nectin-2 delta) also exists. It has the same signal sequence and extracellular domain as Nectin-2 alpha (aa 1 - 338), but differs in the TM segment (21 aa in length) and cytoplasmic region (159 aa in length) (4, 6, 7). Mouse Nectin-2 ECD (aa 32 - 338) shares 72%, 77% and 95% aa identity with the ECD in human, canine and rat Nectin-2, respectively. Nectin-2 is known to bind pseudorabies virus, and herpes simplex virus-2 (HSV-2). It also binds select HSV-1 strains. It does not bind poliovirus (1, 10, 11). As a cell adhesion molecule, Nectin-2 will form cis-homodimers (same cell) and trans-homodimers (across cells). Nectin-2 will not cis-dimerize with other Nectins, but will trans-heterodimerize with Nectin-3 and CD266/DNAM-1 (1, 3, 11, 12, 13). Nectin-2 is found concentrated at cell-to-cell interfaces, and is presumed to contribute to tight and adherens junction formation (14). Through its interaction with NK and T cell expressed DNAM-1, it also promotes lymphocyte cytotoxicity and cytokine secretion against both tumors and dendritic cells (DC) expressing Nectin-2 (15, 16). In the case of DC, this may be a mechanism whereby the immune system eliminates DC that are inefficient at antigen presentation. Nectin-2 is expressed on epithelium, endothelial cells, Sertoli cells, monocytes, dendritic cells, granulosa cells, mast cells, eosinophils and fibroblasts.

References

  1. Takai, Y. and H. Nakanishi (2003) J. Cell Sci. 116:17.
  2. Rikitake, Y. and Y. Takai (2008) Cell. Mol. Life Sci. 65:253.
  3. Sasisaka, T. et al. (2007) Curr. Opin. Cell Biol. 19:1.
  4. Aoki, J. et al. (1994) J. Biol. Chem. 269:8431.
  5. Takahashi, K. et al. (1999) J. Cell Biol. 145:539.
  6. Aoki, J. et al. (1997) Exp. Cell Res. 235:374.
  7. Lopez, M. et al. (1998) Blood 92:4602.
  8. Morrison, M.E. and V.R. Racaniello (1992) J. Virol. 66:2807.
  9. Struyf, F. et al. (2002) J. Virol. 76:12940.
  10. Delboy, M.G. et al. (2006) Virology J. 3:105.
  11. Irie, K. et al. (2004) Semin. Cell Dev. Biol. 15:643.
  12. Tahara-Hanaoka, S. et al. (2004) Int. Immunol. 16:533.
  13. Satoh-Horikawa, K. et al. (2000) J. Biol. Chem. 275:10291.
  14. Nakanishi, H. and Y. Takai (2004) Biol. Chem. 385:885.
  15. Tahara-Hanaoka, S. et al. (2006) Blood 107:1491.
  16. Pende, D. et al. (2006) Blood 107:2030.

Long Name

Poliovirus Receptor Related 2

Alternate Names

CD112, HVEB, MPH, Nectin2, PRR2, PVRL2

Entrez Gene IDs

5819 (Human); 19294 (Mouse); 102137456 (Cynomolgus Monkey)

Gene Symbol

NECTIN2

UniProt

Additional Nectin-2/CD112 Products

Product Documents for Recombinant Mouse Nectin-2/CD112 Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse Nectin-2/CD112 Protein, CF

For research use only

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