The vial is supposed to contain lyophilized protein but it appears to be empty. Is there anything in it?

Pellets can be dislodged during shipping and become disbursed on the vial wall and in the cap. Centrifuge or tap the vial on the benchtop to return this material to the vial bottom. If this does not reveal a pellet, closely inspect the cone of the vial. Some pellets appear as only a tiny amount of material or as a transparent film due to the original buffer formulation. This is a normal appearance for many proteins. For example, if the product is originally lyophilized from a solvent such as acetonitrile or ethanol, and supplied carrier-free, you may not be able to detect the pellet with the naked eye. This does not mean the vial is empty. Reconstitute the vial as directed. After reconstitution, protein concentration can be tested with a spectrophotometer.

What is the recommended method for reconstitution of a lyophilized protein or antibody?

Unless more specific directions are on the Certificate of Analysis provided with the product, we suggest the following procedure to ensure optimal recovery: 1. Allow the vial and reconstitution buffer to equilibrate to room temperature. 2. Briefly centrifuge the vial to ensure that all lyophiliate is collected at the bottom of the vial. 3. Add the amount of buffer required to achieve the concentration recommended on the product insert. 4. Allow the vial to reconstitute for 15-30 minutes at room temperature with gentle agitation, like on a rocker platform or rotating by hand. Avoid vigorous shaking that can cause foaming and protein denaturation. 5. Aliquot into volumes greater than 20 μL and store as indicated on the product insert. If the vial exhibits flakes or particulates, mix the product for a couple of hours at room temperature and then at 4oC overnight. Contact Technical Service if product does not go into solution.

Are R&D Systems recombinant proteins and antibodies sterile?

Although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Recombinant Mouse PD-L1/B7-H1 His-tag Protein, CF

Name: Recombinant Mouse PD-L1/B7-H1 His-tag Protein, CF
Brand: R&D Systems
SKU: 9048-B7
Rating: 5 (13 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 9048-B7

Citations (2)

(13)

Product Datasheet / COA / SDS

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Setting the standard in quality research reagents

Key Product Details

Source:	NS0
Accession #:	Q9EP73
Applications:	Bioactivity

View Full Technical Details for Recombinant Mouse PD-L1/B7-H1 His-tag Protein, CF

Product Specifications for Recombinant Mouse PD-L1/B7-H1 His-tag Protein, CF

Source	Mouse myeloma cell line, NS0-derived mouse PD-L1/B7-H1 protein Phe19-His239, with a C-terminal 6-His tag
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Phe19
Predicted Molecular Mass	26 kDa
SDS-PAGE	49-62 kDa, reducing conditions
Activity	Measured by its binding ability in a functional ELISA. When Recombinant Mouse PD-L1/B7-H1 is immobilized at 25 ng/mL (100 µL/well), the concentration of Recombinant Mouse PD-1 Fc Chimera (Catalog # 1021-PD) that produces 50% of the optimal binding response is approximately 8-40 ng/mL

Scientific Data Examples for Recombinant Mouse PD-L1/B7-H1 His-tag Protein, CF

	Recombinant Mouse PD-L1/B7-H1 His-tag Protein Binding Activity When Recombinant Mouse PD-L1/B7-H1 is immobilize at 25 ng/mL, it binds Recombinant Mouse PD-1 Fc Chimera (Catalog # 1021-PD) with an ED₅₀ of 8-40 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9048-B7

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 400 μg/mL in PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PD-L1/B7-H1

B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules (1). Mature mouse B7-H1 consists of a 221 amino acid (aa) extracellular domain (ECD) with two immunoglobulin-like domains, a 21 aa transmembrane segment, and a 30 aa cytoplasmic domain (2). Within the ECD, mouse B7-H1 shares 73% and 86% aa sequence identity with human and rat B7-H1, respectively. B7-H1 is expressed on inflammatory-activated immune cells including macrophages, T cells, and B cells (2-5), keratinocytes (6, 7), enothelial and intestinal epithelial cells (6, 8), as well as a variety of carcinomas and melanoma (9, 10). B7-H1 binds to T cell B7-1/CD80 and PD-1 (5, 6, 10-13). It suppresses T cell activation and proliferation (3, 6, 12, 14) and induces the apoptosis of activated T cells (9). It plays a role in the development of immune tolerance by promoting T cell anergy (5, 12) and enhancing regulatory T cell development (14). B7-H1 favors the development of anti-inflammatory IL-10 and IL-22 producing dendritic cells (3, 8) and inhibits the development of Th17 cells (14). In cancer, B7-H1 provides resistance to T cell mediated lysis, enhances EMT, and enhances the tumorigenic function of Th22 cells (4, 7, 10, 13).

Item	Value
References	Ceeraz, S. et al. (2013) Trends Immunol. 34:556. Tamura, H. et al. (2001) Blood 97:1809. Chen, L. et al. (2007) J. Immunol. 178:6634. Kuang, D.-M. et al. (2014) J. Clin. Invest. 124:4657. Tsushima, F. et al. (2007) Blood 110:180. Mazanet, M.M. and C.C.W. Hughes (2002) J. Immunol. 169:3581. Cao, Y. et al. (2010) Cancer Res. 71:1235. Scandiuzzi, L. et al. (2014) Cell Rep. 6:625. Dong, H. et al. (2002) Nat. Med. 8:793. Azuma, T. et al. (2008) Blood 111:3635. Butte, M.J. et al. (2008) Mol. Immunol. 45:3567. Park, J.-J. et al. (2010) Blood 116:1291. Ritprajak, P. et al. (2010) J. Immunol. 184:4918. Herold, M. et al. (2015) J. Immunol. 195:3584.
Long Name	Programmed Death Ligand 1
Entrez Gene IDs	29126 (Human); 60533 (Mouse); 499342 (Rat); 574058 (Porcine); 484186 (Canine); 102145573 (Cynomolgus Monkey)
Alternate Names	Avelumab, B7-H, B7-H1, B7H1, B7H1PDCD1L1, CD274, CD274 antigenMGC142294, CD274 molecule, PD-L1, PD-L1B7 homolog 1, PDCD1L1, PDCD1LG1, PDCD1LG1MGC142296, PDL1, PDL1PDCD1 ligand 1, Programmed death ligand 1, programmed cell death 1 ligand 1

Citations for Recombinant Mouse PD-L1/B7-H1 His-tag Protein, CF (2)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse PD-L1/B7-H1 His-tag Protein, CF include:

Species: Transgenic Mouse
Sample Types: Whole Cell
Applications: Bioassay

E Cai et al. (2022), T cells use distinct topographical and membrane receptor scanning strategies that individually coalesce during receptor recognition Proceedings of the National Academy of Sciences of the United States of America, 119(32):e2203247119.
PMID: 35914144
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

S Du et al. (2018), Blockade of Tumor-Expressed PD-1 promotes lung cancer growth Oncoimmunology, 7(4):e1408747.
PMID: 29632720

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Customer Reviews for Recombinant Mouse PD-L1/B7-H1 His-tag Protein, CF (13)

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Recombinant Mouse PD-L1/B7-H1 His-tag Protein, CF

Name: Anonymous

Application: In vitro bioactivity in cell culture

Verified Customer | Posted 12/21/2018
Recombinant Mouse PD-L1/B7-H1 His-tag Protein, CF

Name: Anonymous

Application: Immunoassay Standard

Verified Customer | Posted 08/26/2018
Recombinant Mouse PD-L1/B7-H1 His-tag Protein, CF

Name: Anonymous

Application: Binding assay/Protein-protein interaction

Verified Customer | Posted 04/12/2018
Recombinant Mouse PD-L1/B7-H1 His-tag Protein, CF

Name: Anonymous

Application: In vivo study

Verified Customer | Posted 03/30/2018
Recombinant Mouse PD-L1/B7-H1 His-tag Protein, CF

Name: Anonymous

Application: SDS-PAGE Control

Verified Customer | Posted 03/15/2018
Recombinant Mouse PD-L1/B7-H1 His-tag Protein, CF

Name: Anonymous

Application: SDS-PAGE Control

Verified Customer | Posted 03/11/2018
Recombinant Mouse PD-L1/B7-H1 His-tag Protein, CF

Name: Amit Kumar

Application: Binding assay/Protein-protein interaction

Verified Customer | Posted 12/18/2017
Recombinant Mouse PD-L1/B7-H1 His-tag Protein, CF

Name: Anonymous

Application: Binding assay/Protein-protein interaction

Verified Customer | Posted 11/27/2017
Recombinant Mouse PD-L1/B7-H1 His-tag Protein, CF

Name: Anonymous

Application: Binding assay/Protein-protein interaction

Verified Customer | Posted 11/18/2017
Recombinant Mouse PD-L1/B7-H1 His-tag Protein, CF

Name: Anonymous

Application: Binding assay/Protein-protein interaction

Verified Customer | Posted 11/15/2017
Recombinant Mouse PD-L1/B7-H1 His-tag Protein, CF

Name: Anonymous

Application: SDS-PAGE Control

Verified Customer | Posted 09/08/2017
Recombinant Mouse PD-L1/B7-H1 His-tag Protein, CF

Name: Anonymous

Application: Cell Adhesion

Verified Customer | Posted 07/20/2017
Recombinant Mouse PD-L1/B7-H1 His-tag Protein, CF

Name: Anonymous

Application: SPR chip ligand immobilization for kinetic studies

Verified Customer | Posted 07/14/2017

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FAQs for Recombinant Mouse PD-L1/B7-H1 His-tag Protein, CF

The vial is supposed to contain lyophilized protein but it appears to be empty. Is there anything in it?

Pellets can be dislodged during shipping and become disbursed on the vial wall and in the cap. Centrifuge or tap the vial on the benchtop to return this material to the vial bottom. If this does not reveal a pellet, closely inspect the cone of the vial. Some pellets appear as only a tiny amount of material or as a transparent film due to the original buffer formulation. This is a normal appearance for many proteins. For example, if the product is originally lyophilized from a solvent such as acetonitrile or ethanol, and supplied carrier-free, you may not be able to detect the pellet with the naked eye. This does not mean the vial is empty. Reconstitute the vial as directed. After reconstitution, protein concentration can be tested with a spectrophotometer.
What is the recommended method for reconstitution of a lyophilized protein or antibody?

Unless more specific directions are on the Certificate of Analysis provided with the product, we suggest the following procedure to ensure optimal recovery: 1. Allow the vial and reconstitution buffer to equilibrate to room temperature. 2. Briefly centrifuge the vial to ensure that all lyophiliate is collected at the bottom of the vial. 3. Add the amount of buffer required to achieve the concentration recommended on the product insert. 4. Allow the vial to reconstitute for 15-30 minutes at room temperature with gentle agitation, like on a rocker platform or rotating by hand. Avoid vigorous shaking that can cause foaming and protein denaturation. 5. Aliquot into volumes greater than 20 μL and store as indicated on the product insert. If the vial exhibits flakes or particulates, mix the product for a couple of hours at room temperature and then at 4^oC overnight. Contact Technical Service if product does not go into solution.
Are R&D Systems recombinant proteins and antibodies sterile?

Although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Recombinant Mouse PD-L1/B7-H1 His-tag Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Mouse PD-L1/B7-H1 His-tag Protein, CF

Scientific Data Examples for Recombinant Mouse PD-L1/B7-H1 His-tag Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: PD-L1/B7-H1

Citations for Recombinant Mouse PD-L1/B7-H1 His-tag Protein, CF (2)

Customer Reviews for Recombinant Mouse PD-L1/B7-H1 His-tag Protein, CF (13)

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Recombinant Mouse PD-L1/B7-H1 His-tag Protein, CF

Recombinant Mouse PD-L1/B7-H1 His-tag Protein, CF

Recombinant Mouse PD-L1/B7-H1 His-tag Protein, CF

Recombinant Mouse PD-L1/B7-H1 His-tag Protein, CF

Recombinant Mouse PD-L1/B7-H1 His-tag Protein, CF

Recombinant Mouse PD-L1/B7-H1 His-tag Protein, CF

Recombinant Mouse PD-L1/B7-H1 His-tag Protein, CF

Recombinant Mouse PD-L1/B7-H1 His-tag Protein, CF

Recombinant Mouse PD-L1/B7-H1 His-tag Protein, CF

Recombinant Mouse PD-L1/B7-H1 His-tag Protein, CF

Recombinant Mouse PD-L1/B7-H1 His-tag Protein, CF

Recombinant Mouse PD-L1/B7-H1 His-tag Protein, CF

Recombinant Mouse PD-L1/B7-H1 His-tag Protein, CF

FAQs for Recombinant Mouse PD-L1/B7-H1 His-tag Protein, CF

Product Documents for Recombinant Mouse PD-L1/B7-H1 His-tag Protein, CF

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