Recombinant Mouse PD-L1/B7-H1 His-tag Protein, CF
R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Source: | NS0 |
Accession #: | Q9EP73 |
Applications: | Bioactivity |
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Citations for Recombinant Mouse PD-L1/B7-H1 His-tag Protein, CF (2)
Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse PD-L1/B7-H1 His-tag Protein, CF include:
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Species: Transgenic Mouse
Sample Types: Whole Cell
Applications: BioassayE Cai et al. (2022), T cells use distinct topographical and membrane receptor scanning strategies that individually coalesce during receptor recognition Proceedings of the National Academy of Sciences of the United States of America, 119(32):e2203247119.
PMID: 35914144 -
Species: Mouse
Sample Types: Whole Cells
Applications: BioassayS Du et al. (2018), Blockade of Tumor-Expressed PD-1 promotes lung cancer growth Oncoimmunology, 7(4):e1408747.
PMID: 29632720
There are no citations that match your criteria.
Customer Reviews for Recombinant Mouse PD-L1/B7-H1 His-tag Protein, CF (13)
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Application: In vitro bioactivity in cell cultureVerified Customer | Posted 12/21/2018
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Application: Immunoassay StandardVerified Customer | Posted 08/26/2018
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Application: Binding assay/Protein-protein interactionVerified Customer | Posted 04/12/2018
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Application: In vivo studyVerified Customer | Posted 03/30/2018
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Application: SDS-PAGE ControlVerified Customer | Posted 03/15/2018
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Application: SDS-PAGE ControlVerified Customer | Posted 03/11/2018
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Application: Binding assay/Protein-protein interactionVerified Customer | Posted 12/18/2017
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Application: Binding assay/Protein-protein interactionVerified Customer | Posted 11/27/2017
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Application: Binding assay/Protein-protein interactionVerified Customer | Posted 11/18/2017
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Application: Binding assay/Protein-protein interactionVerified Customer | Posted 11/15/2017
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Application: SDS-PAGE ControlVerified Customer | Posted 09/08/2017
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Application: Cell AdhesionVerified Customer | Posted 07/20/2017
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Application: SPR chip ligand immobilization for kinetic studiesVerified Customer | Posted 07/14/2017
There are no reviews that match your criteria.
FAQs for Recombinant Mouse PD-L1/B7-H1 His-tag Protein, CF
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The vial is supposed to contain lyophilized protein but it appears to be empty. Is there anything in it?
Pellets can be dislodged during shipping and become disbursed on the vial wall and in the cap. Centrifuge or tap the vial on the benchtop to return this material to the vial bottom. If this does not reveal a pellet, closely inspect the cone of the vial. Some pellets appear as only a tiny amount of material or as a transparent film due to the original buffer formulation. This is a normal appearance for many proteins. For example, if the product is originally lyophilized from a solvent such as acetonitrile or ethanol, and supplied carrier-free, you may not be able to detect the pellet with the naked eye. This does not mean the vial is empty. Reconstitute the vial as directed. After reconstitution, protein concentration can be tested with a spectrophotometer.
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What is the recommended method for reconstitution of a lyophilized protein or antibody?
Unless more specific directions are on the Certificate of Analysis provided with the product, we suggest the following procedure to ensure optimal recovery: 1. Allow the vial and reconstitution buffer to equilibrate to room temperature. 2. Briefly centrifuge the vial to ensure that all lyophiliate is collected at the bottom of the vial. 3. Add the amount of buffer required to achieve the concentration recommended on the product insert. 4. Allow the vial to reconstitute for 15-30 minutes at room temperature with gentle agitation, like on a rocker platform or rotating by hand. Avoid vigorous shaking that can cause foaming and protein denaturation. 5. Aliquot into volumes greater than 20 μL and store as indicated on the product insert. If the vial exhibits flakes or particulates, mix the product for a couple of hours at room temperature and then at 4oC overnight. Contact Technical Service if product does not go into solution.
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Are R&D Systems recombinant proteins and antibodies sterile?
Although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.