Recombinant Mouse PD-1 Fc Chimera Protein, CF
R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Source: | NS0 |
Accession #: | Q02242 |
Structure / Form: | Disulfide-linked homodimer |
Applications: | Binding Activity |
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Citations for Recombinant Mouse PD-1 Fc Chimera Protein, CF (7)
Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse PD-1 Fc Chimera Protein, CF include:
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Species: Rat
Sample Types: In Vivo
Applications: Surface Plasmon ResonanceJ Guiteras et al. (2021), Dual and Opposite Costimulatory Targeting with a Novel Human Fusion Recombinant Protein Effectively Prevents Renal Warm Ischemia Reperfusion Injury and Allograft Rejection in Murine Models International Journal of Molecular Sciences, 22(3).
PMID: 33530581 -
Species: Mouse
Sample Types: Protein
Applications: BioassayCJ Edwards et al. (2021), The multi-specific VH-based Humabody CB213 co-targets PD1 and LAG3 on T cells to promote anti-tumour activity British Journal of Cancer, 0(0).
PMID: 34969998 -
Species: Mouse
Sample Types: Plasma
Applications: ELISA StandardL Jacobs et al. (2020), DNA-Based Delivery of Checkpoint Inhibitors in Muscle and Tumor Enables Long-Term Responses with Distinct Exposure Mol. Ther., 0(0).
PMID: 32101701 -
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA CaptureN Silva-Pili et al. (2020), Long-Term Systemic Expression of a Novel PD-1 Blocking Nanobody from an AAV Vector Provides Antitumor Activity without Toxicity Biomedicines, 8(12).
PMID: 33276580 -
Species: Mouse
Sample Types: Protein
Applications: Enzyme AssayMatsumura et al. (2015), Botulinum toxin A complex exploits intestinal M cells to enter the host and exert neurotoxicity. Nat Commun, 6(0):6255.
PMID: 25687350 -
Species: Mouse
Sample Types: Whole Cells
Applications: Flow CytometryArnett HA et al. (2007), BTNL2, a butyrophilin/B7-like molecule, is a negative costimulatory molecule modulated in intestinal inflammation. J. Immunol., 178(3):1523-33.
PMID: 17237401 -
Species: Mouse
Sample Types: Whole Cells
Applications: BioassayButte MJ et al. (2007), Programmed death-1 ligand 1 interacts specifically with the B7-1 costimulatory molecule to inhibit T cell responses. Immunity, 27(1):111-22.
PMID: 17629517
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Customer Reviews for Recombinant Mouse PD-1 Fc Chimera Protein, CF (4)
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Application: Binding assay/Protein-protein interactionVerified Customer | Posted 12/02/2021
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Application: Binding assay/Protein-protein interactionVerified Customer | Posted 11/08/2021
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Application: Binding assay/Protein-protein interactionVerified Customer | Posted 10/25/2021
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Application: Binding assay/Protein-protein interactionVerified Customer | Posted 02/11/20190.5 ug mPD-1-hIgG1 (Cat# 1021-PD-100) was added to cells expressing mPD-L1 mCherry. Binding was detected with anti-human 488 secondary antibody.
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FAQs for Recombinant Mouse PD-1 Fc Chimera Protein, CF
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The vial is supposed to contain lyophilized protein but it appears to be empty. Is there anything in it?
Pellets can be dislodged during shipping and become disbursed on the vial wall and in the cap. Centrifuge or tap the vial on the benchtop to return this material to the vial bottom. If this does not reveal a pellet, closely inspect the cone of the vial. Some pellets appear as only a tiny amount of material or as a transparent film due to the original buffer formulation. This is a normal appearance for many proteins. For example, if the product is originally lyophilized from a solvent such as acetonitrile or ethanol, and supplied carrier-free, you may not be able to detect the pellet with the naked eye. This does not mean the vial is empty. Reconstitute the vial as directed. After reconstitution, protein concentration can be tested with a spectrophotometer.
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What is the recommended method for reconstitution of a lyophilized protein or antibody?
Unless more specific directions are on the Certificate of Analysis provided with the product, we suggest the following procedure to ensure optimal recovery: 1. Allow the vial and reconstitution buffer to equilibrate to room temperature. 2. Briefly centrifuge the vial to ensure that all lyophiliate is collected at the bottom of the vial. 3. Add the amount of buffer required to achieve the concentration recommended on the product insert. 4. Allow the vial to reconstitute for 15-30 minutes at room temperature with gentle agitation, like on a rocker platform or rotating by hand. Avoid vigorous shaking that can cause foaming and protein denaturation. 5. Aliquot into volumes greater than 20 μL and store as indicated on the product insert. If the vial exhibits flakes or particulates, mix the product for a couple of hours at room temperature and then at 4oC overnight. Contact Technical Service if product does not go into solution.
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Are R&D Systems recombinant proteins and antibodies sterile?
Although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.