Product Specifications for Recombinant Mouse Osteocrin Protein, CF
E. coli-derived mouse Osteocrin protein Val28-Gly130, with a C-terminal 6-His tag
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
14-18 kDa, reducing conditions
Measured by its binding ability in a functional ELISA. When Recombinant Mouse NPRC/NPR3 Fc Chimera (Catalog # 10187-NR) is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Mouse Osteocrin that produces 50% of the optimal binding response is 0.6-3.6 ng/mL.
Scientific Data Examples for Recombinant Mouse Osteocrin Protein, CF
Recombinant Mouse Osteocrin Protein Binding Activity
When Recombinant Mouse NPRC/NPR-3 Fc Chimera (Catalog # 10187-NR) is immobilized at 2 µg/mL, Recombinant Mouse Osteocrin (Catalog # 9700-ON) binds with an ED50 of 0.6-3.6 ng/mL.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitute at 250 μg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Osteocrin, also known as Musclin, is a secreted protein that is primarily expressed in bone and muscle. It is synthesized as a proprotein (11 kDa) that undergoes proteolytic processing to generate a mature 50 amino acid C-terminal active peptide (1). It was found to modulate osteoblast differentation and to regulate glucose metabolism in muscles (2). Mouse Osteocrin proprotein shares 77% and 78% amino acid sequence identity with the rat and human protein, respectively. Secretion of Osteocrin has been shown to be increased with exercise and is associated with metabolically beneficial formation of brown fat (3). Osteocrin has also been attributed to increases in exercise endurance through promotion of mitochondrial biosynthesis (4). Based on similarities with NPs (natriuretic peptides), Osteocrin was found to interact with NP clearance receptor NPR-C. This interaction is thought to modulate the availability of NPs, importantly increasing CNP which has been shown to stimulate endochondral ossification and elongate bones (5). In primates, Osteocrin may have evolved to regulate neuronal structure and function (6).
Nishizawa, H. et al. (2004) Journal of Biological Chemistry, 279:19391.
Thomas, G. et al. (2003) Journal of Biological Chemistry, 278:50563.
Jeramic N. et al. (2017) J Cell Physiol 232:61.
Subbatino, E. et al. (2015) Proc. Acad. Natl. Sci. U. S. A. 112:16042.
Kanai, Y. et al. (2017) J of Clin Invest 127: 4136
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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