Recombinant Mouse NPRC/NPR3 Fc Chimera Protein, CF

Name: Recombinant Mouse NPRC/NPR3 Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 10187-NR
Price: 636 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 10187-NR

Citations (1)

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Key Product Details

Source:	NS0
Accession #:	P70180
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Mouse NPRC/NPR3 Fc Chimera Protein, CF

Product Specifications for Recombinant Mouse NPRC/NPR3 Fc Chimera Protein, CF

Source

Mouse myeloma cell line, NS0-derived mouse NPRC/NPR3 protein

Mouse NPRC/NPR3 (Glu41-Ala478) Accession # P70180	IEGRMDP	Mouse IgG_2a (Glu98-Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Glu41

Predicted Molecular Mass

78 kDa

SDS-PAGE

76-96 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.

When Recombinant Mouse NPRC/NPR3 Fc Chimera is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Mouse Osteocrin (Catalog # 9700-ON) that produces 50% of the optimal binding response is 0.6-3.6 ng/mL.

Scientific Data Examples for Recombinant Mouse NPRC/NPR3 Fc Chimera Protein, CF

	Recombinant Mouse NPRC/NPR3 Fc Chimera Protein Binding Activity When Recombinant Mouse NPRC/NPR3 Fc Chimera (Catalog # 10187-NR) is immobilized at 2 μg/mL, 100 μL/well, Recombinant Mouse Osteocrin (Catalog # 9700-ON) binds with an ED₅₀ of 0.6-3.6 ng/mL.
	Recombinant Mouse NPRC/NPR3 Fc Chimera Protein SDS-PAGE 2 μg/lane of Recombinant Mouse INPRC/NPR3 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at 76-96 kDa and 170-200 kDa, respectively.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10187-NR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NPRC/NPR3

Atrial Natriuretic Peptide Receptor-3 (NPR3), also known as NPR-C or ANPR-C, is one of the three natriuretic peptide receptors (1, 2). Mouse NPR3 is a type I transmembrane glycoprotein that contains a 438 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 37 aa cytoplasmic region. The natriuretic system is key to the maintenance of vascular tone and cardiovascular homeostasis. It consists of three related natriuretic peptides: atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), and C-type natriuretic peptide (CNP). It also consists of three single-membrane-spanning receptors (NPRs) to mediate the biological activity of these peptides: NPR1, NPR2, and NPR3. NPR1 and NPR2 are guanylyl cyclase receptors that regulate cGMP levels, while NPR3 lacks enzymatic activity and may act as a clearance receptor (1, 2). Both ANP and BNP exhibit high binding affinities to NPR1 and NPR3, while CNP binds with high affinity to NPR2 and NPR3 (2). NPR3 is known to be expressed in the heart, lung, adrenal gland, heart, cerebral cortex, cerebellum, liver and adipocytes and in some cancers (1, 2). Osteocrin was found to be a specific ligand to NPR3 (3). NPR3 is necessary for Osteocrin to regulate femoral, tibial, and metatarsal bone elongation (4).

Item	Value
References	Potter, L.R. et al. (2006) Endocr. Rev. 27:47. Koller, K. J. and D. V. Goeddel (1992) Circulation 86:1081. Thomas, G. et al. (2003) J. Biol. Chem. 278:50563. Moffatt, P. et al. (2007) J. Biol. Chem. 282:36454.
Long Name	Natriuretic Peptide Receptor C
Entrez Gene IDs	4883 (Human); 18162 (Mouse); 25339 (Rat)
Alternate Names	ANP-C, ANPR-C, ANPRCFLJ14054, Atrial natriuretic peptide clearance receptor, Atrial natriuretic peptide receptor type C, C5orf23, GUCY2B, NPR-C, NPR3, NPRC, atrial natriuretic peptide receptor 3, chromosome 5 open reading frame 23, lgj, natriuretic peptide receptor C/guanylate cyclase C (atrionatriuretic peptidereceptor C), stri

Citations for Recombinant Mouse NPRC/NPR3 Fc Chimera Protein, CF (1)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse NPRC/NPR3 Fc Chimera Protein, CF include:

Species: Mouse
Sample Types: Recombinant Proteins
Applications: Bioassay

M Szaroszyk et al. (2022-01-01), Skeletal muscle derived Musclin protects the heart during pathological overload Nature Communications, 13(1):149.
PMID: 35013221

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