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Recombinant Mouse IL-27 (NS0-expressed) Protein Best Seller

Bio-Techne includes R&D Systems | Catalog # 2799-ML

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Carrier Free
2799-ML-010/CF

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With Carrier
2799-ML-010

Key Product Details

Source

NS0

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse IL-27 protein
Mouse EBI3
(Tyr19-Pro228)
Accession # O35228
GGGSGGGSGGGSGGGS Mouse p28
(Phe29-Ser234)
Accession # Q8K3I6
6-His tag
N-terminus C-terminus

Purity

>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Tyr19

Predicted Molecular Mass

48.7 kDa

SDS-PAGE

60-65 kDa, reducing conditions

Activity

Measured in an anti-viral assay using HepG2 human hepatocellular carcinoma cells infected with encephalomyocarditis (EMC) virus. Bender H. et al. (2009) Hepatology 50:585.
The ED50 for this effect is 0.6-6 ng/mL.

Scientific Data Images for Recombinant Mouse IL-27 (NS0-expressed) Protein

Recombinant Mouse IL-27 (NS0-expressed) Protein Bioactivity

Recombinant Mouse IL-27 (NS0-expressed) Protein Bioactivity

Recombinant Mouse IL-27 (Catalog # 2799-ML) demonstrates anti-viral activity in HepG2 human hepatocellular carcinoma cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 0.6-6 ng/mL.
Recombinant Mouse IL-27 (NS0-expressed) Protein SDS-PAGE

Recombinant Mouse IL-27 (NS0-expressed) Protein SDS-PAGE

1 μg/lane of Recombinant Mouse IL-27 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 61 kDa.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 2799-ML
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and CHAPS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 2799-ML/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and CHAPS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-27

IL-27 is a heterodimeric group 2 receptor ligand molecule that belongs to the IL-6/IL-12 family of long type I cytokines (1). It is composed of EBI3 (EBV-induced gene 3), a 34 kDa glycoprotein that is related to the p40 subunit of IL-12 and IL-23, and p28, the cloned 28 kDa glycoprotein that is related to the p35 chain of IL-12 (2-4). The mouse EBI3 gene encodes a 228 amino acid (aa) precursor that contains an 18 aa signal peptide and a 210 aa mature protein (5). The mature region contains two potential N-linked glycosylation sites, two fibronectin type III domains, and two pairs of conserved cysteine residues that place the molecule in the type I cytokine receptor family (5). As with p40, (the EBI3 counterpart in IL-12), IL-27 EBI3 is reported to form homodimers (6). Mouse EBI3 is 61% and 66% aa identical to human and bovine EBI3. The mouse p28 gene encodes a 234 aa precursor that contains a 28 aa signal sequence and a 206 aa mature region (7). The mature region is characterized by the presence of one potential N-linked glycosylation site and four alpha-helices, placing it in the IL-6 family of helical cytokines. Mouse p28 is 74% aa identical to human p28. IL-27 is expressed by monocytes, endothelial cells and dendritic cells (8). IL-27 binds to and signals through a heterodimeric receptor complex composed of WSX-1 (TCCR) and gp130. Evidence suggests IL-27 interacts only with WSX-1 (7, 9, 10). IL-27 has both anti- and proinflammatory properties. As an anti-inflammatory, IL-27 seems to induce a general negative feedback program that limits T and NK-T cell activity (3, 8). At the onset of infection, IL-27 induces an IL-12 receptor on naïve CD4+ T cells, making them susceptible to subsequent IL-12 activity (and possible Th1 development) (6). Notably, IL-12 family cytokines are both induced and inhibited by bacterial products. Microbes promote IL-27 secretion through TLR4, and also block IL-27 production via C5a induction (11).

References

  1. Boulay, J-L. et al. (2003) Immunity 19:159.
  2. Trinchieri, G. et al. (2003) Immunity 19:641.
  3. Murakami, M. et al. (2004) Growth Factors 22:75.
  4. Cordoba-Rodriguez, R. and D.M. Frucht (2003) Expert Opin. Biol. Ther. 3:715.
  5. Nomura, H. et al. (1997) GenBank Accession # O35228.
  6. Holscher, C. (2004) Med. Microbiol. Immunol. (Berl). 193:1.
  7. Pflanz, S. et al. (2002) Immunity 16:779.
  8. Villarino, A.V. et al. (2004) J. Immunol. 173:715.
  9. Pflanz, S. et al. (2004) J. Immunol. 172: 2225.
  10. Scheller, J. et al. (2005) Biochem. Biophys. Res. Commun. 326:724.
  11. Hawlish, H. et al. (2005) Immunity 22:415.

Long Name

Interleukin 27

Alternate Names

IL27

Entrez Gene IDs

246778 (Human); 246779 (Mouse); 102121242 (Cynomolgus Monkey)

Gene Symbol

IL27

Additional IL-27 Products

Product Documents for Recombinant Mouse IL-27 (NS0-expressed) Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse IL-27 (NS0-expressed) Protein

For research use only

Citations for Recombinant Mouse IL-27 (NS0-expressed) Protein (39)

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  • Recombinant Mouse IL-27 (NS0-expressed) Protein
    Name: Anonymous
    Application: Stem/Immune cell maintenance or differentiation
    Verified Customer | Posted 03/17/2020
    Recombinant Mouse IL-27 (NS0-expressed) Protein 2799-ML
  • Recombinant Mouse IL-27 (NS0-expressed) Protein
    Name: Anonymous
    Application: Immunoassay Standard
    Verified Customer | Posted 12/02/2019
    Recombinant Mouse IL-27 (NS0-expressed) Protein 2799-ML
  • Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 06/15/2018
  • Recombinant Mouse IL-27 (NS0-expressed) Protein
    Name: Mahesh Raundhal
    Application: T cell differentiation
    Verified Customer | Posted 04/13/2017
    Naive (CD4+CD44+CD62L++CD25-) mouse T cells were stimulated with anti-CD3, anti-CD28, IL-2 and 50 ng/mL IL-27. IFNg and IL-10 secretion was analyzed in the culture supernatants 5 days later by multiplex assays.
    Recombinant Mouse IL-27 (NS0-expressed) Protein 2799-ML

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