Recombinant Mouse IL-15 Protein

Name: Recombinant Mouse IL-15 Protein
Brand: R&D Systems
SKU: 447-ML
Rating: 5 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 447-ML

Citations (50)

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Key Product Details

Source:	E. coli
Accession #:	P48346
Applications:	Bioactivity

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Product Specifications for Recombinant Mouse IL-15 Protein

Source	E. coli-derived mouse IL-15 protein Asn49-Ser162, with an N-terminal Met
Purity	>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin level	<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Met
Predicted Molecular Mass	13.4 kDa
Activity	Measured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells. The ED₅₀ for this effect is 2-15 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

447-ML

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in Tris and NaCl with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

447-ML/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in Tris and NaCl.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: IL-15

Interleukin 15 (IL-15) is a widely expressed 14 kDa cytokine that is structurally and functionally related to IL-2 and plays an important role in many immunological diseases (1, 2). Mature mouse IL-15 shares 70% and 96% amino acid sequence identity with human and rat IL-15, respectively. IL-15 binds with high affinity to IL‑15 R alpha (3). It binds with lower affinity to a complex of IL‑2 R beta and the common gamma chain ( gamma c) which are also subunits of the IL-2 receptor complex (4). IL-15 associates with IL-15 R alpha in the endoplasmic reticulum, and this complex is expressed on the cell surface (5). The dominant mechanism of IL-15 action is known as transpresentation in which IL-15 and IL-15 R alpha are coordinately expressed on the surface of one cell and interact with complexes of IL-2 R beta / gamma c on adjacent cells (6). This enables cells to respond to IL-15 even if they do not express IL-15 R alpha (5). In human and mouse, soluble IL-15-binding forms of IL-15 R alpha can be generated by proteolytic shedding and bind up nearly all the IL-15 in circulation (7-9). Soluble IL-15 R alpha functions as an inhibitor that limits IL-15 action (3, 8). Ligation of membrane‑associated IL-15/IL-15 R alpha complexes also induces reverse signaling that promotes activation of the IL-15/IL-15 R alpha expressing cells (10). IL-15 induces or enhances the differentiation, maintenance, or activation of multiple T cell subsets including NK, NKT, Th17, Treg, and CD8⁺ memory cells (11-15). An important component of these functions is the ability of IL-15 to induce dendritic cell differentiation and inflammatory activation (10, 13). IL-15 exhibits anti-tumor activity independent of its actions on NK cells or CD8⁺ T cells (16). It also inhibits the deposition of lipid in adipocytes, and its circulating levels are decreased in obesity (17).

Item	Value
References	De Sabatino, A. et al. (2011) Cytokine Growth Factor Rev. 22:19. Grabstein, K. et al. (1994) Science 264:965. Giri, J.G. et al. (1995) EMBO J. 14:3654. Giri, J. et al. (1994) EMBO J. 13:2822. Dubois, S. et al. (2002) Immunity 17:537. Castillo, E.F. and K.S. Schluns (2012) Cytokine 59:479. Budagian, V. et al. (2004) J. Biol. Chem. 279:40368. Mortier, E. et al. (2004) J. Immunol. 173:1681. Bergamaschi, C. et al. (2012) Blood 120:e1. Budagian, V. et al. (2004) J. Biol. Chem. 279:42192. Mortier, E. et al. (2003) J. Exp. Med. 205:1213. Gordy, L.E. et al. (2011) J. Immunol. 187:6335. Harris, K.M. (2011) J. Leukoc. Biol. 90:727. Xia, J. et al. (2010) Clin. Immunol. 134:130. Schluns, K.S. et al. (2002) J. Immunol. 168:4827. Davies, E. et al. (2010) J. Leukoc. Biol. 88:529. Barra, N.G. et al. (2010) Obesity 18:1601.
Long Name	Interleukin 15
Entrez Gene IDs	3600 (Human); 16168 (Mouse); 25670 (Rat); 102119613 (Cynomolgus Monkey); 493682 (Feline)
Alternate Names	IL-15, IL-15MGC9721, IL15, interleukin 15, interleukin-15

Citations for Recombinant Mouse IL-15 Protein (50)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse IL-15 Protein include:

Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

PL Vu et al. (2022), A Mutated Prostatic Acid Phosphatase (PAP) Peptide-Based Vaccine Induces PAP-Specific CD8+ T Cells with Ex Vivo Cytotoxic Capacities in HHDII/DR1 Transgenic Mice Cancers, 14(8).
PMID: 35454873
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

GM Wiedemann et al. (2021), Deconvoluting global cytokine signaling networks in natural killer cells Nature Immunology, 0(0).
PMID: 33859404
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

K Hashimoto et al. (2021), Induction of alopecia areata in C3H/HeJ mice using cryopreserved lymphocytes Journal of dermatological science, 0(0).
PMID: 33994063
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

P Prakhar et al. (2021), The small intestine epithelium exempts Foxp3+ Tregs from their IL-2 requirement for homeostasis and effector function JCI Insight, 6(21).
PMID: 34747370
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture

A Barbarin et al. (2020), Innate T-&alpha&beta lymphocytes as new immunological components of anti-tumoral "off-target" effects of the tyrosine kinase inhibitor dasatinib Sci Rep, 10(1):3245.
PMID: 32094501
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

GM Wiedemann et al. (2020), Divergent Role for STAT5 in the Adaptive Responses of Natural Killer Cells Cell Reports, 33(11):108498.
PMID: 33326784
Species: Mouse
Sample Types: In Vivo
Applications: Bioassay

SS Ng et al. (2020), The NK cell granule protein NKG7 regulates cytotoxic granule exocytosis and inflammation Nat. Immunol., 0(0).
PMID: 32839608
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

N Simonovi? et al. (2019), NK Cells Require Cell-Extrinsic and -Intrinsic TYK2 for Full Functionality in Tumor Surveillance and Antibacterial Immunity J. Immunol., 0(0).
PMID: 30718299
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

X Yang et al. (2019), Enhanced cytotoxic T lymphocytes recruitment targeting tumor vasculatures by endoglin aptamer and IP-10 plasmid presenting liposome-based nanocarriers Theranostics, 9(14):4066-4083.
PMID: 31281532
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

S Xiao et al. (2018), Thymic epithelial cell-derived signals control B progenitor formation and proliferation in the thymus by regulating Let-7 and Arid3a PLoS ONE, 13(2):e0193188.
PMID: 29462197
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

HF Moffett et al. (2017), The microRNA miR-31 inhibits CD8(+) T cell function in chronic viral infection Nat. Immunol., 0(0).
PMID: 28530712
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

A Van Acker et al. (2017), A Murine Intestinal Intraepithelial NKp46-Negative Innate Lymphoid Cell Population Characterized by Group 1 Properties Cell Rep, 19(7):1431-1443.
PMID: 28514662
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

I Kavazovi? et al. (2017), NKG2D stimulation of CD8+ T cells during priming promotes their capacity to produce cytokines in response to viral infection in mice Eur. J. Immunol., 0(0).
PMID: 28378389
Species: Human
Sample Types: Protein
Applications: Surface Plasmon Resonance

AP Vicari et al. (2017), Discovery and characterization of a novel humanized anti-IL-15 antibody and its relevance for the treatment of refractory celiac disease and eosinophilic esophagitis MAbs, 0(0):0.
PMID: 28581883
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

P Holmkvist et al. (2016), IL-18R?-deficient CD4(+) T cells induce intestinal inflammation in the CD45RB(hi) transfer model of colitis despite impaired innate responsiveness Eur J Immunol, 0(0).
PMID: 27062602
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Fabrizio Antonangel (2016), Natural killer cell recognition of in vivo drug-induced senescent multiple myeloma cells Oncoimmunology, 5(10):e1218105.
PMID: 27853638
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

David A Hildeman (2016), Temporal Expression of Bim Limits the Development of Agonist-Selected Thymocytes and Skews Their TCR? Repertoire J. Immunol., 0(0).
PMID: 27852740
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

RB Jones et al. (2016), A Subset of Latency-Reversing Agents Expose HIV-Infected Resting CD4+ T-Cells to Recognition by Cytotoxic T-Lymphocytes PLoS Pathog, 12(4):e1005545.
PMID: 27082643
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Pobezinsky L et al. (2015), Let-7 microRNAs target the lineage-specific transcription factor PLZF to regulate terminal NKT cell differentiation and effector function. Nat Immunol, 16(5):517-24.
PMID: 25848867
Species: Mouse
Sample Types: Whole Organism
Applications: In vivo

Kurtulus S et al. (2015), Bim controls IL-15 availability and limits engagement of multiple BH3-only proteins. Cell Death Differ, 22(1):174-84.
PMID: 25124553
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Harris T et al. (2015), Drak2 Does Not Regulate TGF-beta Signaling in T Cells. PLoS ONE, 10(5):e0123650.
PMID: 25951457
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Fiege J et al. (2015), Negative Regulation of Memory Phenotype CD8 T Cell Conversion by Adhesion and Degranulation-Promoting Adapter Protein. J Immunol, 195(7):3119-28.
PMID: 26320248
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Celis-Gutierrez J et al. (2014), Dok1 and Dok2 proteins regulate natural killer cell development and function. EMBO J, 33(17):1928-40.
PMID: 24963146
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Krupnick A et al. (2014), Central memory CD8+ T lymphocytes mediate lung allograft acceptance. J Clin Invest, 124(3):1130-43.
PMID: 24569377
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Lee F et al. (2014), The effects of CAMPATH-1H on cell viability do not correlate to the CD52 density on the cell surface. PLoS ONE, 9(7):e103254.
PMID: 25050704
Species: Mouse
Sample Types: Whole Blood
Applications: Bioassay

Dowty et al. (2014), Preclinical to clinical translation of tofacitinib, a Janus kinase inhibitor, in rheumatoid arthritis. J Pharmacol Exp Ther, 348(1):165-73.
PMID: 24218541
Species: N/A
Sample Types: N/A
Applications: Western Blot

Pennati A et al. (2014), Maltose-binding protein fusion allows for high level bacterial expression and purification of bioactive mammalian cytokine derivatives. PLoS ONE, 9(9):e106724.
PMID: 25198691
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Marcais A et al. (2014), The metabolic checkpoint kinase mTOR is essential for IL-15 signaling during the development and activation of NK cells. Nat Immunol, 15(8):749-57.
PMID: 24973821
Species: Mouse
Sample Types: In Vivo

Quinn L et al. (2014), IL-15 is required for postexercise induction of the pro-oxidative mediators PPARdelta and SIRT1 in male mice. Endocrinology, 155(1):143-55.
PMID: 24169546
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Zarling S et al. (2013), The survival of memory CD8 T cells that is mediated by IL-15 correlates with sustained protection against malaria. J Immunol, 190(10):5128-41.
PMID: 23589611
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Golden-Mason L et al. (2013), Galectin-9 functionally impairs natural killer cells in humans and mice. J Virol, 87(9):4835-45.
PMID: 23408620
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Dudda J et al. (2013), MicroRNA-155 is required for effector CD8+ T cell responses to virus infection and cancer. Immunity, 38(4):742-53.
PMID: 23601686
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Ahmed Rahim M et al. (2013), Normal development and function but impaired memory phenotype of CD8(+) T cells in transgenic mice expressing HIV-1 Nef in its natural target cells. Virology, 438(2):84-97.
PMID: 23433958
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

Sneddon et al. (2012), Self-renewal of embryonic-stem-cell-derived progenitors by organ-matched mesenchyme. Nature, 491(7426):765-8.
PMID: 23041930
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

(2012), Regulation of innate CD8+ T-cell activation mediated by cytokines. Proc Natl Acad Sci U S A, 109(25):9971-6.
PMID: 22665806
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Michel M et al. (2012), Interleukin 7 (IL-7) selectively promotes mouse and human IL-17-producing gammadelta cells. Proc Natl Acad Sci U S A, 109(43):17549-54.
PMID: 23047700
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Kurtulus S et al. (2011), Bcl-2 allows effector and memory CD8+ T cells to tolerate higher expression of Bim. J. Immunol., 186(10):5729-37.
PMID: 21451108
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Verbist KC et al. (2011), A role for IL-15 in the migration of effector CD8 T cells to the lung airways following influenza infection. J. Immunol., 186(1):174-82.
PMID: 21098221
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

DePaolo RW et al. (2011), Co-adjuvant effects of retinoic acid and IL-15 induce inflammatory immunity to dietary antigens. Nature, 471(7337):220-4.
PMID: 21307853
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Kayamuro H et al. (2010), Interleukin-1 family cytokines as mucosal vaccine adjuvants for induction of protective immunity against influenza virus. J. Virol., 84(24):12703-12.
PMID: 20881038
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Barra NG et al. (2010), Interleukin-15 contributes to the regulation of murine adipose tissue and human adipocytes. Obesity (Silver Spring), 18(8):1601-7.
PMID: 20019685
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Tripathi P et al. (2010), STAT5 is critical to maintain effector CD8+ T cell responses. J. Immunol., 185(4):2116-24.
PMID: 20644163
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Pepper M et al. (2010), Different routes of bacterial infection induce long-lived TH1 memory cells and short-lived TH17 cells. Nat. Immunol., 11(1):83-9.
PMID: 19935657
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Sikora AG et al. (2009), IFN-alpha enhances peptide vaccine-induced CD8+ T cell numbers, effector function, and antitumor activity. J. Immunol., 182(12):7398-407.
PMID: 19494262
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Pan W et al. (2008), Permeation of blood-borne IL15 across the blood-brain barrier and the effect of LPS. J. Neurochem., 106(1):313-9.
PMID: 18384647
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Wan N et al. (2008), Bystander central memory but not effector memory CD8+ T cells suppress allograft rejection. J. Immunol., 180(1):113-21.
PMID: 18097010
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Zaph C et al. (2008), Commensal-dependent expression of IL-25 regulates the IL-23-IL-17 axis in the intestine. J. Exp. Med., 205(10):2191-8.
PMID: 18762568
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Rafei M et al. (2007), A GMCSF and IL-15 fusokine leads to paradoxical immunosuppression in vivo via asymmetrical JAK/STAT signaling through the IL-15 receptor complex. Blood, 109(5):2234-42.
PMID: 17082320
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Zheng SG et al. (2007), IL-2 is essential for TGF-beta to convert naive CD4+CD25- cells to CD25+Foxp3+ regulatory T cells and for expansion of these cells. J. Immunol., 178(4):2018-27.
PMID: 17277105
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Ferretti S et al. (2003), IL-17, produced by lymphocytes and neutrophils, is necessary for lipopolysaccharide-induced airway neutrophilia: IL-15 as a possible trigger. J. Immunol., 170(4):2106-12.
PMID: 12574382

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Customer Reviews for Recombinant Mouse IL-15 Protein (1)

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Name: Katie Myers

Application: Immunoassay Standard

Reason for Rating: Seemed well-purified and worked for our purposes.

Verified Customer | Posted 11/14/2022

We used this as a standard for a mouse ELISA.

Recombinant Mouse IL-15 Protein

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Complete Your Research

Complete Your Experiment

Product Specifications for Recombinant Mouse IL-15 Protein

Formulation, Preparation and Storage

Carrier Free

447-ML

Carrier

447-ML/CF

Carrier Free

Reconstitution Calculator

Background: IL-15

Citations for Recombinant Mouse IL-15 Protein (50)

Customer Reviews for Recombinant Mouse IL-15 Protein (1)

Customer Images

Product Documents for Recombinant Mouse IL-15 Protein

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