Recombinant Mouse HVEM/TNFRSF14 Fc Chimera Protein, CF

Name: Recombinant Mouse HVEM/TNFRSF14 Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 2516-HV
Price: 417 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 2516-HV

Citations (1)

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Key Product Details

Source:	CHO
Accession #:	NP_849262
Structure / Form:	Disulfide-linked homodimer
Applications:	Binding Activity

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Product Specifications for Recombinant Mouse HVEM/TNFRSF14 Fc Chimera Protein, CF

Source

Chinese Hamster Ovary cell line, CHO-derived mouse HVEM/TNFRSF14 protein

Mouse HVEM (Gln39-Val207) Accession # NP_849262	IEGRMDP	Mouse IgG_2A (Glu98-Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Gln39

Predicted Molecular Mass

45.6 kDa

SDS-PAGE

60-65 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Mouse (rm) HVEM Fc Chimera is immobilized at 100 ng/ mL, 100 μL/well, the concentration of biotinylated rmBTLA Fc Chimera that produces 50% of the optimal binding response is found to be approximately 30-120 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2516-HV

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 1 mg/mL in PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HVEM/TNFRSF14

HVEM (herpesvirus entry mediator), also known as TNFRSF14 and CD270, is a type I membrane protein in the TNF receptor superfamily, and it can both promote and inhibit T cell activity (1). Mature mouse HVEM consists of a 170 amino acid (aa) extracellular domain (ECD) with three cysteine-rich domains (CRD), a 24 aa transmembrane segment, and a 45 aa cytoplasmic tail with a TRAF interaction domain (2). Within the ECD, mouse HVEM shares 55% and 89% aa sequence identity with human and rat HVEM, respectively. HVEM is highly expressed on naïve CD4⁺ T cells, CD8⁺ T memory cells, regulatory T cells, dendritic cells, monocytes, and neutrophils (3-7). Its expression declines during effector T cell activation but is upregulated during Treg activation (3, 4). HVEM functions as a receptor for BTLA, CD160, LIGHT/TNFSF14, and Lymphotoxin-alpha (3, 8-11). Ligation of HVEM by LIGHT triggers T cell, monocyte, and neutrophil activation (7, 9) and contributes to Th1 inflammation and cardiac allograft rejection (12, 13). In contrast, HVEM binding to CD160 or BTLA suppresses T cell and dendritic cell activation (3, 6, 8, 9) and dampens intestinal inflammation (14). HVEM enhances the development of CD8⁺ T cell memory and Treg function (4, 5). It is additionally expressed on intestinal epithelial cells, where its binding by intraepithelial lymphocyte (IEL) expressed CD160 promotes epitheilal integrity and host defense (15). The herpesvirus envelope glycoprotein gD, which binds HVEM to initiate membrane fusion, can antagonize both BTLA and LIGHT binding (Montgomery, 8, 10, 16).

Item	Value
References	del Rio, M.L. et al. (2010) J. Leukoc. Biol. 87:223. Hsu, H. et al. (1997) J. Biol. Chem. 272:13471. Sedy, J. R. et al. (2005) Nat. Immunol. 6:90. Tao, R. et al. (2008) J. Immunol. 180:6649. Steinberg, M.W. et al. (2013) PLoS One 8:e77992. de Trez, C. et al. (2008) J. Immunol. 180:238. Heo, S.K. et al. (2006) J. Leukoc. Biol. 79:330. Gonzalez, L.C. et al. (2005) Proc. Natl. Acad Sci. USA 102:1116. Cai, G. et al. (2008) Nat. Immunol. 9:176. Mauri, D.N. et al. (1998) Immunity 8:21. Harrop, J.A. et al. (1998) J. Biol. Chem. 273:27548. Wang, J. et al. (2005) J. Immunol. 174:8173. Ye, Q. et al. (2002) J. Exp. Med. 195:795. Steinberg, M.W. et al. (2008) J. Exp. Med. 205:1463. Shui, J.W. et al. (2012) Nature 488:222. Montgomery, R.I. et al. (1996) Cell 87:427.
Long Name	Herpesvirus Entry Mediator
Entrez Gene IDs	8764 (Human); 230979 (Mouse); 102137807 (Cynomolgus Monkey)
Alternate Names	ATAR, CD270, CD270 antigen, CD40-like protein, HVEM, HVEMTR2HVEAATAR, Herpes virus entry mediator A, Herpesvirus entry mediator A, HveA, LIGHTR, TNFRSF14, Tumor necrosis factor receptor-like 2, tumor necrosis factor receptor superfamily member 14, tumor necrosis factor receptor superfamily, member 14 (herpesvirus entrymediator), tumor necrosis factor receptor-like gene2

Citations for Recombinant Mouse HVEM/TNFRSF14 Fc Chimera Protein, CF (1)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse HVEM/TNFRSF14 Fc Chimera Protein, CF include:

Species: N/A
Sample Types: Beads
Applications: Bead-based Bioassay

H Kinosada et al. (2017-01-01), HTLV-1 bZIP Factor Enhances T-Cell Proliferation by Impeding the Suppressive Signaling of Co-inhibitory Receptors PLoS Pathog, 13(1):e1006120.
PMID: 28046066

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Recombinant Mouse HVEM/TNFRSF14 Fc Chimera Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

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