Recombinant Mouse CEACAM-5/CD66e His-tag Protein, CF

Name: Recombinant Mouse CEACAM-5/CD66e His-tag Protein, CF
Brand: rnd
SKU: 10404-CM

Catalog # 10404-CM

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Key Product Details

Source:	NS0
Accession #:	Q3UKK2.1
Applications:	Bioactivity

View Full Technical Details for Recombinant Mouse CEACAM-5/CD66e His-tag Protein, CF

Technical Data
Citations / Reviews / FAQs

Product Specifications

Source	Mouse myeloma cell line, NS0-derived mouse CEACAM-5/CD66e protein Gln35-Glu947, with a C-terminal 6-His tag
Purity	>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Gln35
Predicted Molecular Mass	103 kDa
SDS-PAGE	115-135 kDa, under reducing conditions
Activity	Measured by its binding ability in a functional ELISA. When Recombinant Mouse CEACAM-5/CD66e His-tag (Catalog # 10404-CM) is immobilized at 0.5 µg/mL (100 µL/well), Recombinant Mouse Galectin-4 (Catalog # 2128-GA) binds with an ED50 of 0.1-0.9 μg/mL.

Recombinant Mouse CEACAM-5/CD66e His-tag Protein, CF Scientific Data Examples

	Recombinant Mouse CEACAM-5/CD66e His-tag Protein Binding Activity When Recombinant Mouse CEACAM/CD66e His-tag (10404-CM) is immobilized at 0.5 μg/mL (100 μL/well), Recombinant Mouse Galectin-4 (2128-GA) binds with an ED₅₀ of 0.1-0.9 μg/mL.
	Recombinant Mouse CEACAM-5/CD66e His-tag Protein SDS-PAGE 2 μg/lane of Recombinant Mouse CEACAM-5/CD66e His-tag (10404-CM) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 115-135 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10404-CM

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 400 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CEACAM-5/CD66e

CEACAM-5, also known as CEA, CD66e and Psg30, belongs to the large family of CEACAM and pregnancy specific glycoproteins. CEACAM family members are highly glycosylated with varying arrangements of IgV-like and IgC-like regions in their extracellular domains (ECD) and can be expressed as transmembrane, glycophosphatidyl inositol (GPI) linked or soluble proteins (1-3). CEACAM-5 consists of an N-terminal Ig-like V-set domain followed by six Ig-like C2-set domains and a GPI anchor (2, 4, 5). While the mature ECD of mouse CEACAM-5 shares 26% amino acid identity with human CEACAM-5, it remains unclear if these molecules are direct orthologs (6). CEACAM-5, expressed primarily by epithelial cells, functions as a calcium-independent adhesion molecule through homophilic and heterophilic interactions with CEACAM-1 (1, 7). CEACAM-5 is restricted to the apical face of intestinal epithelial cells in the adult but is more diffuse during embryonic development and in tumors (8). This is consistent with a role in the development and maintenance of epithelial architecture. CEACAM-5 is up-regulated in a wide variety of human tumors, promoting tumor cell migration, invasion, adhesion, and metastasis, and has been used as a cancer marker (8, 9). It also contributes to tumor formation by maintaining cellular proliferation in the presence of differentiation stimuli, and by blocking apoptosis following loss of ECM anchorage (anoikis) (10, 11). The GPI anchoring of CEACAM-5 can be released by GPI-PLD, resulting in a soluble molecule that also promotes tumor metastasis (12). Cell surface expression of CEACAM-5 on tumor cells prevents the adhesion of CEACAM-1 expressing NK cells and provides protection from NK-mediated lysis (8). CEACAM-5 was shown to bind galectin-4 and by surface plasmon resonance and coimmunoprecipitated with galectin-4 in human colon adenocarcinoma LS174T cell lysates (13). CEACAM-5 also binds a subset of Neisseria opacity proteins (Opa) and E. coli adhesion proteins (14, 15). These interactions trigger clustering of the lipid raft-localized CEACAM-5 to sites of pathogen contact (15, 16).

Item	Value
References	Zebhauser, R. et al. (2005) Genomics 86:566. Hammarstrom, S. (1999) Semin. Cancer Biol. 9:67. Chan, et al. (2007) Curr Oncol. 14:70. Schrewe H. et al. (1990) Mol. Cell. Biol. 10:2738. Hefta, S.A. et al. (1988) Proc. Natl. Acad. Sci. 85:4648. Hance KW et al. Mutat Res. (2005) 576:132. Stern, N. et al. (2005) J. Immunol. 174:6692. Beauchemin N. et al. (2013) Cancer Metastasis Rev. 32:3. Blumenthal, R.D. et al. (2005) Cancer Res. 65:8809. Screaton, R.A. et al. (1997) J. Cell Biol. 137:939. Ordonez, C. et al. (2000) Cancer Res. 60:3419. Yamamoto, Y. et al. (2005) Biochem. Biophys. Res. Commun. 333:223. Ideo H. et al. (2005) J Biol Chem. 280:4730. Martin, J.N. et al. (2016) Biochemistry. 55:4286 Berger, C.N. et al. (2004) Mol. Microbiol. 52:963. Tchoupa, A.K. et al. (2014) Cell Commun Signal. 12:27.
Long Name	Carcinoembryonic Antigen-related Cell Adhesion Molecule 5
Entrez Gene IDs	1048 (Human); 73250 (Mouse)
Alternate Names	CD66e, CD66e antigen, CEA, CEACAM-5, CEACAM5, CEACD66e, Carcinoembryonic antigen, DKFZp781M2392, Meconium antigen 100, carcinoembryonic antigen-related cell adhesion molecule 5

Citations

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