Product Specifications for Recombinant Mouse CEACAM-5/CD66e His-tag Protein, CF
Mouse myeloma cell line, NS0-derived mouse CEACAM-5/CD66e protein Gln35-Glu947, with a C-terminal 6-His tag
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
115-135 kDa, under reducing conditions
Measured by its binding ability in a functional ELISA. When Recombinant Mouse CEACAM-5/CD66e His-tag (Catalog # 10404-CM) is immobilized at 0.5 µg/mL (100 µL/well), Recombinant Mouse Galectin-4 (Catalog # 2128-GA) binds with an ED50 of 0.1-0.9 μg/mL.
Scientific Data Examples for Recombinant Mouse CEACAM-5/CD66e His-tag Protein, CF
Recombinant Mouse CEACAM-5/CD66e His-tag Protein Binding Activity
When Recombinant Mouse CEACAM/CD66e His-tag (10404-CM) is immobilized at 0.5 μg/mL (100 μL/well), Recombinant Mouse Galectin-4 (2128-GA) binds with an ED50 of 0.1-0.9 μg/mL.
Recombinant Mouse CEACAM-5/CD66e His-tag Protein SDS-PAGE
2 μg/lane of Recombinant Mouse CEACAM-5/CD66e His-tag (10404-CM) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 115-135 kDa.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitute at 400 μg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
CEACAM-5, also known as CEA, CD66e and Psg30, belongs to the large family of CEACAM and pregnancy specific glycoproteins. CEACAM family members are highly glycosylated with varying arrangements of IgV-like and IgC-like regions in their extracellular domains (ECD) and can be expressed as transmembrane, glycophosphatidyl inositol (GPI) linked or soluble proteins (1-3). CEACAM-5 consists of an N-terminal Ig-like V-set domain followed by six Ig-like C2-set domains and a GPI anchor (2, 4, 5). While the mature ECD of mouse CEACAM-5 shares 26% amino acid identity with human CEACAM-5, it remains unclear if these molecules are direct orthologs (6). CEACAM-5, expressed primarily by epithelial cells, functions as a calcium-independent adhesion molecule through homophilic and heterophilic interactions with CEACAM-1 (1, 7). CEACAM-5 is restricted to the apical face of intestinal epithelial cells in the adult but is more diffuse during embryonic development and in tumors (8). This is consistent with a role in the development and maintenance of epithelial architecture. CEACAM-5 is up-regulated in a wide variety of human tumors, promoting tumor cell migration, invasion, adhesion, and metastasis, and has been used as a cancer marker (8, 9). It also contributes to tumor formation by maintaining cellular proliferation in the presence of differentiation stimuli, and by blocking apoptosis following loss of ECM anchorage (anoikis) (10, 11). The GPI anchoring of CEACAM-5 can be released by GPI-PLD, resulting in a soluble molecule that also promotes tumor metastasis (12). Cell surface expression of CEACAM-5 on tumor cells prevents the adhesion of CEACAM-1 expressing NK cells and provides protection from NK-mediated lysis (8). CEACAM-5 was shown to bind galectin-4 and by surface plasmon resonance and coimmunoprecipitated with galectin-4 in human colon adenocarcinoma LS174T cell lysates (13). CEACAM-5 also binds a subset of Neisseria opacity proteins (Opa) and E. coli adhesion proteins (14, 15). These interactions trigger clustering of the lipid raft-localized CEACAM-5 to sites of pathogen contact (15, 16).
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