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Recombinant Mouse CD40/TNFRSF5 Fc Chimera Protein, CF

Bio-Techne includes R&D Systems | Catalog # 1215-CD

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1215-CD-050

Key Product Details

Source

NS0

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse CD40/TNFRSF5 protein
Mouse CD40
(Val24-Arg193)
Accession # P27512
DIEGRMD Human IgG1
(Pro100-Lys330)
6-His tag
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Val24

Predicted Molecular Mass

46 kDa (monomer)

SDS-PAGE

58-61 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse CD40/TNFRSF5 Fc Chimera is immobilized at 1 µg/mL (100 µL/well), the concentration of Recombinant Mouse CD40 Ligand/TNFSF5 (Catalog # 8230-CL) that produces 50% optimal binding is found to be 100-600 ng/mL.

Formulation, Preparation and Storage

1215-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD40/TNFRSF5

CD40, also known as TNFRSF5, is a 32 kDa type I transmembrane glycoprotein member of the TNF receptor superfamily. Mature mouse CD40 consists of a 174 amino acid (aa) extracellular domain, a transmembrane domain, and a 74 aa cytoplasmic domain (1, 2). The extracellular domain of mouse CD40 shares 58% and 81% aa sequence identity with human and rat CD40, respectively. Multiple mouse CD40 splice variants have been identified including soluble isoforms. CD40 is expressed on the surface of B cells, dendritic cells, macrophages, monocytes and platelets, as well as endothelial and epithelial cells (3, 4). Interaction of CD40 with its ligand, CD40 Ligand, leads to the aggregation of CD40 molecules resulting in the initiation of intracellular signaling in both CD40 and CD40 Ligand expressing cells (5). CD40 ligation by CD40 Ligand promotes B cell activation and T cell-dependent humoral responses (6, 7). CD40 serves multiple functions in both hematopoietic and epithelial cancers and is a target for tumor immunotherapy (8, 9). Dysregulation of CD40/CD40 Ligand expression and interactions contributes to the immune deficiency associated with HIV infection and AIDS (10, 11). It is also implicated in the pathology of multiple cardiovascular diseases including atherosclerosis, atherothrombosis, and restenosis (12, 13).

 

References

  1. Torres, R.M. and E.A. Clark (1992) J. Immunol. 148:620.
  2. Torres, R.M. et al. (1992) J. Immunol. 149:2641.
  3. van Kooten, C. and J. Banchereau (1997) Curr. Opin. Immunol. 9:330.
  4. Schonbeck, U. et al. (1997) J. Biol. Chem. 272:19569.
  5. Eissner, G. et al. (2004) Cytokine Growth Factor Rev. 15:353.
  6. Rickert, R.C. et al. (2011) Immunol. Rev. 244:115.
  7. Elgueta, R. et al. (2009) Immunol. Rev. 229:152.
  8. Loskog, A.S. and A.G. Eliopoulos (2009) Semin. Immunol. 21:301.
  9. Hangalapura, B.N. et al. (2012) J. Gene Med. 14:416.
  10. Kornbluth, R.S. (2000) J. Leukoc. Biol. 68:373.
  11. Chougnet, C. (2003) J. Leukoc. Biol. 74:702.
  12. Pamukcu, B. et al. (2011) Ann. Med. 43:331.
  13. Hassan, G.S. et al. (2012) Immunobiology 217:521.

Alternate Names

CD40, TNFRSF5

Entrez Gene IDs

958 (Human); 21939 (Mouse); 171369 (Rat); 102118696 (Cynomolgus Monkey)

Gene Symbol

CD40

UniProt

Additional CD40/TNFRSF5 Products

Product Documents for Recombinant Mouse CD40/TNFRSF5 Fc Chimera Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse CD40/TNFRSF5 Fc Chimera Protein, CF

For research use only

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Reconstitution Calculator

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