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Recombinant Mouse CD117/c-kit Fc Chimera Protein, CF

Catalog # 1356-SR | R&D Systems, Inc. a Bio-Techne Brand
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1356-SR-050

Key Product Details

Source

NS0

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse CD117/c-kit protein
Mouse CD117/c-kit
(Gln26-Thr523)
Accession # P05532
IEGRMDP Mouse IgG2A
(Glu98-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln26 predicted: No results obtained, sequencing might be blocked

Predicted Molecular Mass

82.6 kDa (monomer)

SDS-PAGE

100-125 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse CD117/c-kit Fc Chimera is present at 0.5 μg/mL, the concentration of Recombinant Mouse SCF/c‑kit Ligand (Catalog # 455-MC) that produces 50% of the optimal binding response is approximately 3‑12 ng/mL.

Formulation, Preparation and Storage

1356-SR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD117/c-kit

Stem Cell Factor Receptor (SCF R), also known as c‑Kit and CD117, is a widely expressed 145 kDa receptor tyrosine kinase. It is the cellular homolog of the feline sarcoma virus protein, v‑Kit. Binding of SCF R to SCF, also known as Steel Factor and Kit Ligand, promotes the survival, differentiation, and mobilization of progenitor cells in multiple lineages (1‑4). Mutations or deletions of SCF R cause a wide variety of malignancies as well as pigmentation disorders and sterility (5, 6). Mature mouse SCF R consists of a 503 amino acid (aa) extracellular domain (ECD) with five tandem immunoglobulin‑like domains, a 21 aa transmembrane segment, and a 431 aa cytoplasmic domain with the split tyrosine kinase domain (7). Within the ECD, mouse SCF R shares 73% and 88% aa sequence identity with human and rat SCF R, respectively. Alternative splicing of mouse SCF R generates a truncated intracellular isoform that corresponds to the C‑terminal half of the tyrosine kinase domain (8). SCF is expressed as transmembrane and soluble noncovalent homodimers (9). One SCF dimer binds to two molecules of SCF R, inducing receptor dimerization and activation (9). Transmembrane SCF induces more prolonged signaling through SCF R compared to soluble SCF (10). Rat SCF is active on mouse and human cells, but human SCF is only weakly active on mouse cells (11). A 100 kDa glycosylated ECD fragment of SCF R can be shed into the circulation by TACE/ADAM17, and this fragment inhibits the interaction of SCF with transmembrane SCF R (12, 13). SCF is a primary growth and activation factor for mast cells and eosinophils (14). SCF R expression on mast cells enables them to infiltrate SCF‑secreting tumors where they promote tumor growth and induce local immune suppression (15). SCF R is up‑regulated on dendritic cells by Th2‑ or Th17‑biasing stimuli, and it is required for subsequent dendritic cell induction of Th2 and Th17 responses (16). SCF R protects vascular smooth muscle cells from apoptosis and assists in the recovery of cardiac function following myocardial infarction (17, 18).

References

  1. Kimura, Y. et al. (2011) PLoS ONE 6:e26918.
  2. Tallini, Y.N. et al. (2009) Proc. Natl. Acad. Sci. USA 106:1808.
  3. Bashamboo, A. et al. (2006) J. Cell Sci. 119:3039.
  4. Sun, L. et al. (2004) J. Clin. Invest. 113:1364.
  5. Pittoni, P. et al. (2011) Oncogene 30:757.
  6. Sartini, S. et al. (2011) Curr. Med. Chem. 18:2893.
  7. Qiu, F.H. et al. (1988) EMBO J. 7:1003.
  8. Zayas, J. et al. (2008) Stem Cells Dev. 17:343.
  9. Lemmon, M.A. et al. (1997) J. Biol. Chem. 272:6311.
  10. Miyazawa, K. et al. (1995) Blood 85:641.
  11. Martin, F.H. et al. (1990) Cell 63:203.
  12. Turner, A.M. et al. (1995) Blood 85:2052.
  13. Cruz, A.C. et al. (2004) J. Biol. Chem. 279:5612.
  14. Jamur, M.C. and C. Oliver (2011) Front. Biosci. (School Ed.) 3:1390.
  15. Huang, B. et al. (2008) Blood 112:1269.
  16. Krishnamoorthy, N. et al. (2008) Nat. Med. 14:565.
  17. Wang, C.-H. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:540.
  18. Kanellakis, P. et al. (2006) Cardiovasc. Res. 70:117.

Long Name

Stem Cell Factor Receptor

Alternate Names

c-kit, CD117, ckit, KIT, PBT, SCF R, SCFR

Entrez Gene IDs

3815 (Human); 16590 (Mouse); 64030 (Rat)

Gene Symbol

KIT

UniProt

Product Documents for Recombinant Mouse CD117/c-kit Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse CD117/c-kit Fc Chimera Protein, CF

For research use only

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