Recombinant Mouse Cathepsin D Protein, CF

Name: Recombinant Mouse Cathepsin D Protein, CF
Brand: R&D Systems
SKU: 1029-AS
Price: 394 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 1029-AS

Citations (3)

Product Datasheet / COA / SDS

Catalog #

Availability

Size / Price

Qty

Setting the standard in quality research reagents

Key Product Details

Source:	NS0
Accession #:	Q3UCD9
Structure / Form:	Pro form
Applications:	Enzyme Activity

View Full Technical Details for Recombinant Mouse Cathepsin D Protein, CF

Product Specifications for Recombinant Mouse Cathepsin D Protein, CF

Source	Mouse myeloma cell line, NS0-derived mouse Cathepsin D protein Ile21-Leu410, with a C-terminal 10-His tag
Purity	>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin level	<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ile21
Predicted Molecular Mass	44 kDa
SDS-PAGE	52 kDa, reducing conditions
Activity	Measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH₂ (Catalog # ES001). The specific activity is >600 pmol/min/µg, as measured under described conditions.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1029-AS

Formulation	Lyophilized from a 0.2 μm filtered solution in MES and NaCl.
Reconstitution	Reconstitute at 100 μg/mL in sterile, deionized water. Reconstitution Buffer Available: Size / Price Qty Loading... RB07:
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: Cathepsin D

Cathepsin D is a lysosomal aspartic protease of the pepsin family (4). Mouse Cathepsin D is synthesized as a precursor protein, consisting of a signal peptide (residues 1‑20), a propeptide (residues 21‑64), and a mature chain (residues 65‑410) (1‑3). It is expressed in most cells and overexpressed in breast cancer cells (5). It is a major enzyme in protein degradation in lysosomes, and also involved in the presentation of antigenic peptides. Mice deficient in this enzyme showed a progressive atrophy of the intestinal mucosa, a massive destruction of lymphoid organs, and a profound neuronal ceroid lipofucinosis, indicating that Cathepsin D is essential for proteolysis of proteins regulating cell growth and tissue homeostasis (6). Cathepsin D secreted from human prostate carcinoma cells is responsible for the generation of angiostatin, a potent endogeneous inhibitor of angiogenesis (6).

Item	Value
References	Diedrich, et al. (1990) Nucl. Acid Res. 18:7184. Grusby, et al. (1990) Nucl. Acid Res. 18:4008. Hetman, et al. (1994) DNA Cell Biol. 13:419. Conner (2004) in Handbook of Proteolytic Enzymes (Barrett, et al. eds) Elsevier Academic Press, San Diego, p. 43. Rochefort, et al. (2000) Clin. Chim. Acta. 291:157. Tsukuba, et al. (2000) Mol. Cells 10:601.
Entrez Gene IDs	1509 (Human); 13033 (Mouse)
Alternate Names	CPSD, CTSD, Cathepsin D, EC 3.4.23, EC 3.4.23.5, MGC2311, cathepsin D (lysosomal aspartyl protease), lysosomal aspartyl peptidase, lysosomal aspartyl protease, neuronal 10

Citations for Recombinant Mouse Cathepsin D Protein, CF (3)

Showing 1 - 3 of 3 citations Showing All

Filter By:

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse Cathepsin D Protein, CF include:

Species: Mouse
Sample Types: Cell Lysates
Applications: Bioassay

A Skopál et al. (2022), Cathepsin D interacts with adenosine A2A receptors in mouse macrophages to modulate cell surface localization and inflammatory signaling The Journal of Biological Chemistry, 0(0):101888.
PMID: 35367412
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

X Zhou et al. (2017), Lysosomal processing of progranulin Mol Neurodegener, 12(1):62.
PMID: 28835281
Species: Mouse
Sample Types: Recombinant Protein
Applications: Enzyme Assay

Zeeuwen PL et al. (2010), The cystatin M/E-cathepsin L balance is essential for tissue homeostasis in epidermis, hair follicles, and cornea. FASEB J., 24(10):3744-55.
PMID: 20495178

There are no citations that match your criteria.

Showing 1 - 3 of 3 citations Showing All

Product Documents for Recombinant Mouse Cathepsin D Protein, CF

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Recombinant Mouse Cathepsin D Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Customers also Viewed

Customers also Purchased