Recombinant Mouse BAFF/BLyS/TNFSF13B Protein

Name: Recombinant Mouse BAFF/BLyS/TNFSF13B Protein
Brand: R&D Systems
SKU: 8876-BF

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 8876-BF

Citations (24)

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Key Product Details

Source:	NS0
Accession #:	Q9WU72
Structure / Form:	Noncovalently-linked homotrimer
Applications:	Bioactivity

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Product Specifications for Recombinant Mouse BAFF/BLyS/TNFSF13B Protein

Source	Mouse myeloma cell line, NS0-derived mouse BAFF/BLyS/TNFSF13B protein Ala127-Leu309
Purity	>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ala127
Predicted Molecular Mass	21 kDa
SDS-PAGE	19-23 kDa (monomer) & 38-47 kDa (non-reducible dimer), reducing conditions
Activity	Measured in a cell proliferation assay using anti-IgM stimulated mouse B cells. The ED₅₀ for this effect is 0.1‑0.5 ng/mL in the presence of goat anti‑mouse IgM µ chain.

Scientific Data Examples for Recombinant Mouse BAFF/BLyS/TNFSF13B Protein

	Recombinant Mouse BAFF/BLyS/TNFSF13B Protein Bioactivity Recombinant Mouse BAFF/BLyS/TNFSF13B (Catalog # 8876-BF) induces mouse B cell proliferation. The ED₅₀ for this effect is 0.1‑0.5 ng/mL.The ED₅₀ for the two competitors is >5 ng/mL, which is more than 25-fold weaker.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

8876-BF

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in MOPS, NaCl and TCEP with BSA as a carrier protein..
Reconstitution	Reconstitute at 100-500 μg/mL in sterile water.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

8876-BF/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in MOPS, NaCl and TCEP.
Reconstitution	Reconstitute at 100-500 μg/mL in sterile water.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: BAFF/BLyS/TNFSF13B

B-cell activating factor (BAFF), also known as BLyS, TALL-1, THANK, and TNFSF13B, is a 32 kDa transmembrane glycoprotein in the TNF ligand superfamily. It is involved in multiple aspects of immune system regulation, particularly towards B cells (1, 2). Mature mouse BAFF consists of a 47 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 241 aa extracellular domain (ECD) with a stalk region and one TNF-like domain (3, 4). Within aa 127-309 of the ECD, mouse BAFF shares 72% aa sequence identity with human BAFF. It can be expressed as a homotrimer or as a heteromer in association with the related TNFSF member APRIL (4, 5). A 18 kDa fragment containing the TNF-like domain can be released by proteolysis between Arg126 and Ala127 (4). Soluble BAFF is stored intracellularly in neutrophils and released upon inflammatory stimulation (6). Alternative splicing generates an isoform termed deltaBAFF that lacks 19 aa between the proteolytic cleavage site and the TNF-like domain. deltaBAFF can form heteromers with BAFF and negatively regulates BAFF function (7). BAFF is produced by many hematopoietic cell types including by monocytes, macrophages, neutrophils, dendritic cells, and T cells and also by adipocytes (1, 2, 8). Both BAFF and APRIL are functional ligands for the TNF receptor superfamily members BCMA and TACI, and BAFF additionally binds and signals through BAFF R (9, 10). All three receptors are primarily expressed by B cells (10). BAFF plays a critical role in the development and survival of B lineage cells (2, 11, 12). Mice that over-express BAFF exhibit elevated B cell numbers, increased formation and size of germinal centers, and symptoms of autoimmunity (13). Soluble BAFF is elevated in B cell malignancies, autoimmunity, and other immune disorders (1). In addition, BAFF costimulates T cell activation, promotes a Th1 biased immune response, and promotes the expansion of Treg cells (14-16). BAFF also promotes monocyte survival, proinflammatory cytokine secretion, and differentiation to macrophages (17).

Item	Value
References	Lied, G.A. and A. Berstad (2011) Scand. J. Immunol. 73:1. Mackay, F. et al. (2010) Immunol. Rev. 237:205. Moore, P.A. et al. (1999) Science 285:260. Schneider, P. et al. (1999) J. Exp. Med. 189:1747. Roschke, V. et al. (2002) J. Immunol. 169:4314. Scapini, P. et al. (2003) J. Exp. Med. 197:297. Gavin, A.L. et al. (2003) J. Biol. Chem. 278:38220. Alexaki, V.-I. et al. (2009) J. Immunol. 183:5948. Yu, G. et al. (2000) Nat. Immunol. 1:252. Thompson, J.S. et al. (2001) Science 293:2108. Schiemann, B. et al. (2001) Science 293:2111. Litinskiy, M.B. et al. (2002) Nat. Immunol. 3:822. Batten, M. et al. (2000) J. Exp. Med. 192:1453. Huard, B. et al. (2001) J. Immunol. 167:6225. Sutherland, A.P.R. et al. (2005) J. Immunol. 174:5537. Walters, S. et al. (2009) J. Immunol. 182:793. Chang, S.K. et al. (2006) Blood 108:2687.
Long Name	B cell Activating Factor
Entrez Gene IDs	10673 (Human); 24099 (Mouse); 498666 (Rat); 102123214 (Cynomolgus Monkey)
Alternate Names	ApoL related ligand TALL-1, B lymphocyte stimulator, B-cell-activating factor, BAFF, BAFFB-cell activating factor, BLYSB-lymphocyte stimulator, BLyS, CD257, CD257 antigen, DTL, Dendritic cell-derived TNF-like molecule, TALL-1delta BAFF, TALL1, TALL1Delta4 BAFF, THANK, TNF and ApoL-related leukocyte expressed ligand 1, TNF homolog that activates apoptosis, TNF- and APOL-related leukocyte expressed ligand 1, TNFSF13B, TNFSF20, ZTNF4, tumor necrosis factor (ligand) superfamily, member 13b, tumor necrosis factor (ligand) superfamily, member 20, tumor necrosis factor ligand superfamily member 13B, tumor necrosis factor superfamily, member 13b, tumor necrosis factor-like protein ZTNF4

Citations for Recombinant Mouse BAFF/BLyS/TNFSF13B Protein (24)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse BAFF/BLyS/TNFSF13B Protein include:

Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

H Satofuka et al. (2022), Efficient human-like antibody repertoire and hybridoma production in trans-chromosomic mice carrying megabase-sized human immunoglobulin loci Nature Communications, 13(1):1841.
PMID: 35383174
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

J Kim et al. (2021), Regulation of B cell functions by S-nitrosoglutathione in the EAE model Redox Biology, 45(0):102053.
PMID: 34175668
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

CC Chan et al. (2021), A BAFF/APRIL axis regulates obesogenic diet-driven weight gain Nature Communications, 12(1):2911.
PMID: 34006859
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

L Liu et al. (2021), Circulating CD138 enhances disease progression by augmenting autoreactive antibody production in a mouse model of systemic lupus erythematosus The Journal of Biological Chemistry, 0(0):101053.
PMID: 34364875
Species: Mouse
Sample Types: Naive Splenic B-Cells
Applications: Cell Culture

CT Berry et al. (2020), BCR-Induced Ca2+ Signals Dynamically Tune Survival, Metabolic Reprogramming, and Proliferation of Naive B Cells Cell Rep, 31(2):107474.
PMID: 32294437
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture

P Ramezani-R et al. (2020), E3 Ubiquitin Ligase Fbw7 Regulates the Survival of Mature B Cells J. Immunol., 0(0).
PMID: 32005754
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

C Tan et al. (2020), NR4A nuclear receptors restrain B cell responses to antigen when second signals are absent or limiting Nat. Immunol., 0(0).
PMID: 32868928
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

XY Cai et al. (2020), Etanercept Inhibits B Cell Differentiation by Regulating TNFRII/TRAF2/NF-kappaB Signaling Pathway in Rheumatoid Arthritis Front Pharmacol, 11(0):676.
PMID: 32477138
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Y Jiang et al. (2019), Iron-dependent histone 3 lysine 9 demethylation controls B cell proliferation and humoral immune responses Nat Commun, 10(1):2935.
PMID: 31270335
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture

RW Wu et al. (2019), MicroRNA-29a Counteracts Glucocorticoid Induction of Bone Loss through Repressing TNFSF13b Modulation of Osteoclastogenesis Int J Mol Sci, 20(20).
PMID: 31627291
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

E Ragonnaud et al. (2019), Tumor-derived Thymic stromal lymphopoietin expands bone marrow B-cell precursors in circulation to support metastasis Cancer Res., 0(0).
PMID: 31575547
Species: Complex Species Category
Sample Types: Whole Cells
Applications: Bioassay

M Bolger-Mun et al. (2019), Arp2/3 complex-driven spatial patterning of the BCR enhances immune synapse formation, BCR signaling and B cell activation Elife, 8(0).
PMID: 31157616
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

HD Brightbill et al. (2018), NF-?B inducing kinase is a therapeutic target for systemic lupus erythematosus Nat Commun, 9(1):179.
PMID: 29330524
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

NM Schabla et al. (2018), VprBP (DCAF1) Regulates RAG1 Expression Independently of Dicer by Mediating RAG1 Degradation J. Immunol., 0(0).
PMID: 29925675
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

T Matsushita et al. (2018), BAFF inhibition attenuates fibrosis in scleroderma by modulating the regulatory and effector B cell balance Sci Adv, 4(7):eaas9944.
PMID: 30009261
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

A Sang et al. (2018), Innate and adaptive signals enhance differentiation and expansion of dual-antibody autoreactive B cells in lupus Nat Commun, 9(1):3973.
PMID: 30266981
Species: Mouse
Sample Types: Whole Cells
Applications: Stimulation

SW Du et al. (2018), Integrated B Cell, Toll-like, and BAFF Receptor Signals Promote Autoantibody Production by Transitional B Cells J. Immunol., 0(0).
PMID: 30373855
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

S Khoenkhoen et al. (2018), TACI expression and plasma cell differentiation are impaired in the absence of functional I?BNS Immunol. Cell Biol., 0(0).
PMID: 30597621
Species: Mouse

Applications: ELISA (Standard)

A De Riva et al. (2017), Regulation of type 1 diabetes development and B-cell activation in nonobese diabetic mice by early life exposure to a diabetogenic environment PLoS ONE, 12(8):e0181964.
PMID: 28771521
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

J Jellusova et al. (2017), Gsk3 is a metabolic checkpoint regulator in B cells Nat. Immunol, 0(0).
PMID: 28114292
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

S Park et al. (2017), CD11b regulates antibody class switching via induction of AID Mol. Immunol., 87(0):47-59.
PMID: 28407558
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

JT McKay et al. (2017), PD-L2 Regulates B-1 Cell Antibody Production against Phosphorylcholine through an IL-5-Dependent Mechanism J. Immunol., 0(0).
PMID: 28768724
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

M Kuraoka et al. (2017), BCR and Endosomal TLR Signals Synergize to Increase AID Expression and Establish Central B Cell Tolerance Cell Rep, 18(7):1627-1635.
PMID: 28199836
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Peishan Lee (2016), Differing Requirements for MALT1 Function in Peripheral B Cell Survival and Differentiation J. Immunol, 0(0).
PMID: 28031341

Recombinant Mouse BAFF/BLyS/TNFSF13B Protein

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Complete Your Research

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Product Specifications for Recombinant Mouse BAFF/BLyS/TNFSF13B Protein

Scientific Data Examples for Recombinant Mouse BAFF/BLyS/TNFSF13B Protein

Formulation, Preparation and Storage

Carrier Free

8876-BF

Carrier

8876-BF/CF

Carrier Free

Reconstitution Calculator

Background: BAFF/BLyS/TNFSF13B

Citations for Recombinant Mouse BAFF/BLyS/TNFSF13B Protein (24)

Product Documents for Recombinant Mouse BAFF/BLyS/TNFSF13B Protein

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