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Recombinant Mouse 4-1BB/TNFRSF9 Fc Chimera Protein, CF

Bio-Techne includes R&D Systems | Catalog # 937-4B

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937-4B-050

Key Product Details

Source

NS0

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Binding Activity, Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse 4-1BB/TNFRSF9/CD137 protein
Mouse 4-1BB
(Val24-Leu187)
Accession # P20334
DIEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Val24

Predicted Molecular Mass

44.3 kDa (monomer)

SDS-PAGE

62-66 kDa, reducing conditions

Activity

Measured by its ability to block 4-1BBL-induced IL-2 secretion by mouse T cells.
The ED50 for this effect is 0.3-1 µg/mL in the presence of 0.4 µg/mL of recombinant mouse 4-1BBL.

Measured by its ability to bind recombinant mouse 4-1BB Ligand in a functional ELISA.

Formulation, Preparation and Storage

937-4B
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: 4-1BB/TNFRSF9/CD137

4-1BB, also known as CD137 and TNFRSF9, is an approximately 30 kDa transmembrane glycoprotein in the TNF receptor superfamily. 4-1BB functions in the development and activation of multiple immune cells (1). Mature mouse 4-1BB consists of a 164 amino acid (aa) extracellular domain (ECD) with four TNFR cysteine‑rich repeats, a 21 aa transmembrane segment, and a 48 aa cytoplasmic domain (2). Within the ECD, mouse 4-1BB shares 60% and 79% aa sequence identity with human and rat 4-1BB, respectively. 4-1BB is expressed as a disulfide-linked homodimer on various populations of activated T cells including CD4+, CD8+, memory CD8+, NKT, and regulatory T cells (3-6) as well as on myeloid and mast cell progenitors, dendritic cells, mast cells, and bacterially infected osteoblasts (7-10). It binds with high affinity to the transmembrane 4-1BB Ligand/TNFSF9 which is expressed on antigen presenting cells and myeloid progenitor cells (7, 11). This interaction co‑stimulates the proliferation, activation, and/or survival of the 4-1BB expressing cell (3-6, 11). It can also enhance the activation-induced cell death of repetitively stimulated T cells (11). Mice lacking 4-1BB show augmented T cell activation, perhaps due to its absence on regulatory T cells (12). 4-1BB can associate with OX40 on activated T cells, forming a complex that responds to either ligand and inhibits Treg and CD8+ T cell proliferation (13). Reverse signaling through 4-1BB Ligand inhibits the development of dendritic cells, B cells, and osteoclasts (7, 10) but supports mature dendritic cell survival and co‑stimulates the proliferation and activation of mast cells (8, 9). 4‑1BB activation enhances CD8+ T cell and NK cell mediated anti-tumor immunity (14). It also contributes to the development of inflammation in high fat diet-induced metabolic syndrome (15). Soluble forms of 4-1BB and 4-1BB Ligand circulate at elevated levels in the serum of rheumatoid arthritis and hematologic cancer patients, respectively (16, 17).

References

  1. Kwon, B.S. and S.M. Weissman (1989) Proc. Natl. Acad. Sci. USA 86:1963.
  2. Wen, T. et al. (2002) J. Immunol. 168:4897.
  3. Pulle, G. et al. (2006) J. Immunol. 176:2739.
  4. Zheng, G. et al. (2004) J. Immunol. 173:2428.
  5. Kim, D. et al. (2008) J. Immunol. 180:2062.
  6. Lee, S. et al. (2008) Nat. Immunol. 9:917.
  7. Choi, B.K. et al. (2009) J. Immunol. 182:4107.
  8. Nishimoto, H. et al. (2005) Blood 106:4241.
  9. Saito, K. et al. (2004) J. Biol. Chem. 279:13555.
  10. Alderson, M.R. et al. (1994) Eur. J. Immunol. 24:2219.
  11. Lee, S. et al. (2005) J. Immunol. 174:6803.
  12. Ma, B.Y. et al. (2005) Blood 106:2002.
  13. Choi, B.K. et al. (2010) J. Immunol. 185:1404.
  14. Kim, C. et al. (2011) Diabetes 60:3159.
  15. Michel, J. et al. (1998) Eur. J. Immunol. 28:290.
  16. Salih, H.R. et al. (2001) J. Immunol. 167:4059.

Alternate Names

41BB, CD137, ILA, TNFRSF9

Entrez Gene IDs

3604 (Human); 21942 (Mouse); 500590 (Rat); 102127961 (Cynomolgus Monkey)

Gene Symbol

TNFRSF9

UniProt

Additional 4-1BB/TNFRSF9/CD137 Products

Product Documents for Recombinant Mouse 4-1BB/TNFRSF9 Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse 4-1BB/TNFRSF9 Fc Chimera Protein, CF

For research use only

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