Recombinant Influenza A Virus H3N2 Hemagglutinin Protein, CFNew

Name: Recombinant Influenza A Virus H3N2 Hemagglutinin Protein, CF
Brand: R&D Systems
SKU: 10944-HA

Catalog # 10944-HA

Product Datasheet / COA / SDS

His-tag

Order Information

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Key Product Details

Source:	HEK293
Accession #:	Q91MA7.1
Applications:	Bioactivity

View Full Technical Details for Recombinant Influenza A Virus H3N2 Hemagglutinin Protein, CF

Product Specifications

Source	Human embryonic kidney cell, HEK293-derived influenza a virus h3n2 Hemagglutinin protein Gln17-Trp530, with a C-terminal 6-His tag
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	No results obtained: Gln17 predicted
Predicted Molecular Mass	58 kDa
SDS-PAGE	80-90 kDa, under reducing conditions.
Activity	Measured by its binding ability in a functional ELISA. When Recombinant Human Galectin-1 (Catalog # 1152-GA) immobilized at 10.00 μg/mL, 100 μL/well, the concentration of Recombinant Influenza A Virus H3N2 Hemagglutinin His-tag (Catalog # 10944-HA) that produces 50% of the optimal binding response is 0.35-3.50 μg/mL.

Recombinant Influenza A Virus H3N2 Hemagglutinin Protein, CF Scientific Data Examples

	Recombinant Influenza A Virus H3N2 Hemagglutinin His-tag Protein Binding Activity. When Recombinant Human Galectin-1 (1152-GA) immobilized at 10.00 μg/mL, 100 μL/well, the concentration of Recombinant Influenza A Virus H3N2 Hemagglutinin His-tag (Catalog # 10944-HA) that produces 50% of the optimal binding response is 0.35 - 3.50 μg/mL.
	Recombinant Influenza A Virus H3N2 Hemagglutinin His-tag Protein SDS-PAGE. 2 μg/lane of Recombinant Influenza A Virus H3N2 Hemagglutinin His-tag Protein (Catalog # 10944-HA) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 80-90 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10944-HA

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Hemagglutinin

Influenza A (H3N2) has predominated recent influenza seasons, which caused several hospitalizations in many countries (1). H3N2 viruses have been in circulation since the onset of the 1968 pandemic (2). This strain was a reassortant, composed of the six gene segments from H2N2 viruses, but with the hemagglutinin and polymerase basic protein 1 segments derived from an avian source (3, 4). The hemagglutinin protein of the Influenza A virus (strain A/Hong Kong/1/1968 H3N2) is composed of 550 residues, which includes the Hemagglutinin chains HA1 and HA2 (5). Hemagglutinin (HA) and Neuraminidase (NA) and are the two predominant membrane glycoproteins found on the surface of influenza virus. HA is a lectin that binds sialic acid on host cell membrane. NA is a sialic acid hydrolase that specifically clips off terminally located sialic acid on host cell surface. The two proteins are essential for the infectious cycle of the influenza virus. Galectin-1 can bind to the envelope glycoproteins of influenza virus and inhibit viral infectivity, which may be attributed to the multivalent binding and cross-linking activities of galectin-1. Furthermore, galectin-1 may also be explored for targeting other viruses with glycoproteins on their surface (6).

Item	Value
References	Wan, H. et al. (2019) Nat. Microbiol. 4:2216. Krause, J.C. et al. (2012) J. Virol. 86:6334. Kawaoka, Y. et al. (1989) J. Virol. 63:4603. Alymova, I.V. et al. (2011) J. Virol. 85:12324. Brown, E.G. et al. (2001) Proc. Natl. Acad. Sci. USA 98:6883. Yang, M.L. et al. (2011) J. Virol. 85:10010.
Long Name	Hemagglutinin
Entrez Gene IDs	23308115 (Influenza A Virus H1N1)
Alternate Names	HA, Hemagglutinin

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