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Recombinant Influenza A Virus H3N2 Hemagglutinin Protein, CF

Bio-Techne includes R&D Systems | Catalog # 10944-HA

His-tag
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10944-HA-100

Key Product Details

Source

HEK293

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived influenza a virus h3n2 Hemagglutinin protein
Gln17-Trp530, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained: Gln17 predicted

Predicted Molecular Mass

58 kDa

SDS-PAGE

80-90 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human Galectin-1 (Catalog # 1152-GA) immobilized at 10.00 μg/mL, 100 μL/well, the concentration of Recombinant Influenza A Virus H3N2 Hemagglutinin His-tag (Catalog # 10944-HA) that produces 50% of the optimal binding response is 0.35-3.50 μg/mL.

Scientific Data Images for Recombinant Influenza A Virus H3N2 Hemagglutinin Protein, CF

Recombinant Influenza A Virus H3N2 Hemagglutinin His-tag Protein Binding Activity.

When Recombinant Human Galectin-1 (1152-GA) immobilized at 10.00 μg/mL, 100 μL/well, the concentration of Recombinant Influenza A Virus H3N2 Hemagglutinin His-tag (Catalog # 10944-HA) that produces 50% of the optimal binding response is 0.35 - 3.50 μg/mL.

Recombinant Influenza A Virus H3N2 Hemagglutinin His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Influenza A Virus H3N2 Hemagglutinin His-tag Protein (Catalog # 10944-HA) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 80-90 kDa.

Formulation, Preparation and Storage

10944-HA
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Hemagglutinin

Influenza A (H3N2) has predominated recent influenza seasons, which caused several hospitalizations in many countries (1). H3N2 viruses have been in circulation since the onset of the 1968 pandemic (2). This strain was a reassortant, composed of the six gene segments from H2N2 viruses, but with the hemagglutinin and polymerase basic protein 1 segments derived from an avian source (3, 4). The hemagglutinin protein of the Influenza A virus (strain A/Hong Kong/1/1968 H3N2) is composed of 550 residues, which includes the Hemagglutinin chains HA1 and HA2 (5).   Hemagglutinin (HA) and Neuraminidase (NA) and are the two predominant membrane glycoproteins found on the surface of influenza virus. HA is a lectin that binds sialic acid on host cell membrane. NA is a sialic acid hydrolase that specifically clips off terminally located sialic acid on host cell surface. The two proteins are essential for the infectious cycle of the influenza virus.  Galectin-1 can bind to the envelope glycoproteins of influenza virus and inhibit viral infectivity, which may be attributed to the multivalent binding and cross-linking activities of galectin-1. Furthermore, galectin-1 may also be explored for targeting other viruses with glycoproteins on their surface (6).

References

  1. Wan, H. et al. (2019) Nat. Microbiol. 4:2216.
  2. Krause, J.C. et al. (2012) J. Virol. 86:6334.
  3. Kawaoka, Y. et al. (1989) J. Virol. 63:4603.
  4. Alymova, I.V. et al. (2011) J. Virol. 85:12324.
  5. Brown, E.G. et al. (2001) Proc. Natl. Acad. Sci. USA 98:6883.
  6. Yang, M.L. et al. (2011) J. Virol. 85:10010.

Long Name

Hemagglutinin

Alternate Names

HA

Entrez Gene IDs

23308115 (Influenza A Virus H1N1)

Gene Symbol

HA

UniProt

Additional Hemagglutinin Products

Product Documents for Recombinant Influenza A Virus H3N2 Hemagglutinin Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Influenza A Virus H3N2 Hemagglutinin Protein, CF

For research use only

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