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Recombinant Human Galectin-1 Protein Best Seller

Catalog # 1152-GA | R&D Systems, Inc. a Bio-Techne Brand
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Carrier Free
1152-GA-050/CF

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With Carrier
1152-GA-050

Key Product Details

Source

E. coli

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

E. coli-derived human Galectin-1 protein
Ala2-Asp135

Purity

>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala2

Predicted Molecular Mass

15 kDa

SDS-PAGE

11-14 kDa, under reducing conditions.

Activity

Measured by its ability to agglutinate human red blood cells. Hadari, Y.R. et al. (2000) J. Cell Sci. 113:2385.
The ED50 for this effect is 0.5‑3 µg/mL.

Scientific Data Images for Recombinant Human Galectin-1 Protein

Recombinant Human Galectin-1 Protein SDS-PAGE.

2 μg/lane of Recombinant Human Galectin-1 Protein (Catalog # 1152-GA) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 11-14 kDa.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 1152-GA
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and Betamercaptoethanol with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 1152-GA/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and Betamercaptoethanol.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Galectin-1

Galectin-1, gene name LGALS1 (lectin, galactoside-binding, soluble 1), is a 135 amino acid (aa), 14 kDa, pleiotropic, non-glycosylated, monomeric or homodimeric carbohydrate-binding protein of the prototype galectin family (1-3). Galectins lack a classical signal peptide and can be localized to the cytosolic compartments, or secreted via non-classical pathways (1). Secreted Galectin-1 has immunosuppressive and anti-inflammatory properties and suppresses acute and chronic inflammation and autoimmunity. It contributes to negative selection of developing T cells, immunosuppression by regulatory T cells, resolution of the inflammatory response, and inhibition of immune cell migration, inflammatory cytokine production, and mast cell degranulation (1, 2, 4-6). Galectin-1 preferentially binds laminin, fibronectin, 90K/Mac-2BP, CD45, CD43, CD7, CD2, CD3, integrins alpha4 beta1, alpha5 beta1 and alpha4 beta7, and ganglioside GM1 (2, 3). It is produced in a variety of tissues by cells that include endothelial cells, connective tissue fibroblasts, thymic stromal cells, tumor cells, muscle cells, platelets, regulatory T cells, and activated tissue macrophages, B cells, T cells and dendritic cells (2, 3, 6-11). Most of this expression is cytosolic. Human Galectin-1 shares 88% aa sequence identity with mouse, equine and ovine, 90% with rat, and 87% with bovine and porcine Galectin-1.  Endothelial cell surface expression, including tumor endothelial cells, is greatly increased by cell activation (9). Galectin-1 is highly expressed at the maternal-fetal interface and contributes to fetal immune privilege (5, 12). Its immunosuppressive properties appear to also allow tumor cells to evade immune detection (4, 5). It selectively controls T cell survival by inducing apoptosis of activated Th1 and Th17 cells, which express Galectin-1-binding glycans, while promoting Th2 cell survival where glycans are sialylated and less recognized (4, 13). It also induces apoptosis of immature thymocytes (3, 6). Galectin-1 secreted from bone marrow stromal cells aids B lymphocyte development by contributing to pre-B cell integrin adhesion and receptor signaling (3). The dimer form of Galectin-1 also induces neutrophil down‑regulation by inducing cell surface exposure of phosphatidylserine that marks the cell for phagocytosis (14). Galectin-1 can also modulate cell-cell and cell-matrix interactions, and can promote either cell attachment or detachment depending on the cell type and developmental stage (1, 2).

References

  1. Di Lella, S. et al. (2011) Biochemistry 50:7842.
  2. Rabinovich, G.A. and J.M. Ilarregui (2009) Immunol. Rev. 230:144.
  3. Rabinovich, G.A. and M. Vidal. (2011) Curr. Opin. Hematol. 18:443.
  4. Cedeno-Laurent, F. and C.J. Dimitroff (2012) Clin. Immunol. 142:107.
  5. Than, N.G. et al. (2012) Trends Endocrinol. Metab. 23:23.
  6. Rubinstein, N. et al. (2004) Tissue Antigens 64:1.
  7. Courad, P.O. et al. (1989) J. Biol. Chem. 264:1310.
  8. Hirabayashi, J. et al. (1989) Biochim. Biophys. Acta 1008:85.
  9. Thijssen, V.L. et al. (2008) Am. J. Pathol. 172:545.
  10. Nio-Kobayashi, J. et al. (2009) J. Histochem. Cytochem. 57:41.
  11. Dias-Baruffi, M. et al. (2010) Glycobiology 20:507.
  12. Than, N.G. et al. (2008) Proc. Natl. Acad. Sci. USA 105:15819.
  13. Toscano, M.A. et al. (2007) Nat. Immunol. 8:825.
  14. Stowell, S.R. et al. (2009) J. Biol. Chem. 284:4989.

Alternate Names

BHL, Galaptin, Galectin1, GBP, L-14, LGALS1

Entrez Gene IDs

3956 (Human); 16852 (Mouse)

Gene Symbol

LGALS1

UniProt

Product Documents for Recombinant Human Galectin-1 Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Galectin-1 Protein

For research use only

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  • Recombinant Human Galectin-1 Protein
    Name: Anonymous
    Application: growing cells
    Verified Customer | Posted 03/13/2019
    Recombinant Human Galectin-1 Protein 1152-GA
  • Recombinant Human Galectin-1 Protein
    Name: Anonymous
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    Verified Customer | Posted 10/26/2015
    The protein is well preseved and showed the exact size as expected.
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