Recombinant Influenza A Virus H1N1 Hemagglutinin Protein, CF

Catalog # 11202-HA | R&D Systems, Inc. a Bio-Techne Brand
His-tag (Sf21-Expressed)
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Key Product Details

Accession #

AAD17229.1

Source

Sf 21 (baculovirus)

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived influenza a virus h1n1 Hemagglutinin protein
Asp18-Gln529, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asp18

Predicted Molecular Mass

58 kDa

SDS-PAGE

55-70 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human Galectin-1 (Catalog # 1152-GA) immobilized at 10.00 μg/mL, 100 μL/well, the concentration of Recombinant Influenza A Virus H1N1 Hemagglutinin His-tag (Catalog # 11202-HA) that produces 50% of the optimal binding response is 0.500-5.00 μg/mL.

Scientific Data Images for Recombinant Influenza A Virus H1N1 Hemagglutinin Protein, CF

Recombinant Influenza A Virus H1N1 Hemagglutinin His-tag Protein Binding Activity.

Measured by its binding ability in a functional ELISA. When Recombinant Human Galectin-1 (1152-GA) immobilized at 10.00 μg/mL, 100 μL/well, the concentration of Recombinant Influenza A Virus H1N1 Hemagglutinin His-tag Protein (Catalog # 11202-HA) that produces 50% of the binding response is 0.500-5.00 μg/mL.

Recombinant Influenza A Virus H1N1 Hemagglutinin Protein SDS-PAGE.

2 μg/lane of Recombinant Influenza A Virus H1N1 Hemagglutinin Protein (Catalog # 11202-HA) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 55-70 kDa.

Formulation, Preparation and Storage

11202-HA
Formulation Supplied as a 0.2 μm filtered solution in PBS with Trehalose.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Hemagglutinin

Hemagglutinin (HA) and neuraminidase (NA) are major membrane glycoproteins found on the surface of influenza virus. The two proteins are essential for the infectious cycle of the influenza virus. During initial infection, HA binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. HA exists as a homotrimer, with each monomer composed of a globular HA1 domain and the stalk-like HA2 domain. HA has evolved to recognize long alpha2-6 sialylated glycans for efficient transmission in human population.  Although the HA sequence patterns of the 1918 pandemic and 2009 pandemic viruses are similar, they were caused by different H1N1 subtype viruses. The H1N1 1918 HA shares 86% amino acid sequence identity with the H1N1 2009 HA. Inhibiting HA-mediated membrane fusion is a target for influenza therapies. The HA section in the 1918 virus is being currently used to investigate how the 1918 pandemic spread and how a modern pandemic influenza virus would play out in the future.

References

  1. Michal Lazniewski, M. et al. (2018) Briefings in Functional Genomics 17:415.
  2. Sriwilaijaroen, N. and Suzuki, Y. (2012) Proc Jpn Acad Ser B Phys Biol Sci. 88:226.
  3. Chang, Y.J. et al. (2021) Sci Rep 11:8637.
  4. Short, K. et al. (2018) Front. Cell. Infect. Microbiol. 8:343.

Long Name

Hemagglutinin

Alternate Names

HA

Entrez Gene IDs

23308115 (Influenza A Virus H1N1)

Gene Symbol

HA

UniProt

AAD17229.1

Product Documents for Recombinant Influenza A Virus H1N1 Hemagglutinin Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Influenza A Virus H1N1 Hemagglutinin Protein, CF

For research use only

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