Human embryonic kidney cell, HEK293-derived influenza a virus h1n1 Hemagglutinin protein Asp18-Gln529, with a C-terminal 6-His tag
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
68-83 kDa, under reducing conditions.
Measured by its binding ability in a functional ELISA. When Recombinant Human Galectin-1 (Catalog # 1152-GA) immobilized at 10.00 μg/mL, 100 μL/well, the concentration of Recombinant Influenza A Virus H1N1 HA Tag His-tag (Catalog # 10973-HA) that produces 50% of the optimal binding response is 0.8-8.0 μg/mL.
Recombinant Influenza A Virus H1N1 Hemagglutinin Protein, CF Scientific Data Examples
Recombinant Influenza A Virus H1N1 Hemagglutinin His-tag Protein Binding Activity.
When Recombinant Human Galectin-1 (1152-GA) immobilized at 10.00 μg/mL, 100 μL/well, the concentration of Recombinant Influenza A Virus H1N1 Hemagglutinin His-tag Protein (Catalog # 10973-HA) that produces 50% of the optimal binding response is 0.800-8.00 μg/mL.
Recombinant Influenza A Virus H1N1 Hemagglutinin His-tag Protein SDS-PAGE.
2 μg/lane of Recombinant Influenza A Virus H1N1 Hemagglutinin His-tag Protein (Catalog # 10973-HA) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 68-83 kDa.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitute at 500 μg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Hemagglutinin (HA) and neuraminidase (NA) are major membrane glycoproteins found on the surface of influenza virus. The two proteins are essential for the infectious cycle of the influenza virus. During initial infection, HA binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. HA exists as a homotrimer, with each monomer composed of a globular HA1 domain and the stalk-like HA2 domain. HA has evolved to recognize long alpha 2-6 sialylated glycans for efficient transmission in human population. Although the HA sequence patterns of the 1918 pandemic and 2009 pandemic viruses are similar, they were caused by different H1N1 subtype viruses. Inhibiting HA-mediated membrane fusion is a target for influenza therapies.
Michal Lazniewski, M. et al. (2018) Briefings in Functional Genomics 17:415.
Sriwilaijaroen, N. and Suzuki, Y. (2012) Proc. Jpn. Acad. Ser. B. Phys. Biol Sci. 88:226.
Chang, Y.J. et al. (2021) Sci Rep 11:8637.
Entrez Gene IDs
23308115 (Influenza A Virus H1N1)
Citations for Recombinant Influenza A Virus H1N1 Hemagglutinin Protein, CF
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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