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Recombinant Human VIGR/GPR126 Fc Chimera Protein, CF

Catalog # 10577-GP | R&D Systems, Inc. a Bio-Techne Brand
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10577-GP-050

Key Product Details

Source

HEK293

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human VIGR/GPR126 protein
Human VIGF/GPR126
(Cys38-Lys437)
Accession # AAH75798.1
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Cys38

Predicted Molecular Mass

71 kDa

SDS-PAGE

96-111 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Laminin is immobilized at 5 µg/mL (100 µL/well), Recombinant Human VIGR/GPR126 Fc Chimera (Catalog # 10577-GP) binds with an ED50 of 1.5-12 µg/mL.

Scientific Data Images for Recombinant Human VIGR/GPR126 Fc Chimera Protein, CF

Recombinant Human VIGR/GPR126 Fc Chimera Protein Binding Activity.

When Laminin is immobilized at 5 µg/mL (100 µL/well), Recombinant Human VIGR/GPR126 Fc Chimera (Catalog # 10577-GP) binds with an ED50 of 1.5-12 µg/mL.

Recombinant Human VIGR/GPR126 Fc Chimera Protein SDS-PAGE.

2 μg/lane of Recombinant Human VIGR/GPR126 Fc Chimera Protein (Catalog # 10577-GP) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 96-111 kDa and 180-210 kDa, respectively.

Formulation, Preparation and Storage

10577-GP
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VIGR/GPR126

VIGR (Vascular Inducible G Protein-coupled Receptor), also known as ADGRG6, DREG, and GPR126, is a neuronal 7 TM pass (G protein)-coupled receptor (GPCR) involved in myelination and glial and Schwann cell development (1, 2). Human VIGR cDNA encodes a 1221 amino acid (aa) residue membrane protein with a 37 aa signal peptide, a 825 aa extracellular domain (ECD) with 27 potential N-linked glycosylation sites, seven transmembrane segments that span between aa 863 and aa 1113, and a 108 aa residue cytoplasmic domain. Within ECD human VIGR shares 83% aa sequence identity with mouse and rat VIGR. VIGR is essential for the development of diverse organs (1, 2). Type IV collagen, a major constituent of the basement membrane, binds to VIGR and activates its signaling function (3). This interaction stimulated the production of cAMP in rodent Schwann cells, which require VIGR activity to differentiate, and in human embryonic kidney (HEK293) cells expressing exogenous VIGR. Laminin-211 binds a novel laminin-binding domain in VIGR N-terminal fragment between aa 446 and 807 (4). VIGR-Laminin-211 interactions regulate terminal differentiation and myelination by ensuring appropriate levels of cAMP for a given stage of Schwann cell development (4).

References

  1. Rughetti, A. et al. (2005) J. Immunol. 174:7764.
  2. Engelstaedter, V. et al. (2012) BMC Cancer 12:600.
  3. Taylor-Papadimitriou, J. et al. (1999) Biochim. Biophys. Acta 1455:301.
  4. Geng, Y. et al. (2012) Front Oncol. 2:76.
  5. Tanida, S. et al. (2013) J Biol Chem. 288:31842.
  6. Beatson, R. et al. (2016) Nat Immunol. 17:1273.
  7. Piyush, T. et al. (2017) Cell Death Differ. 24:1937.

Long Name

Vascular-inducible G Protein-coupled Receptor

Alternate Names

APG1, DREG, Gm222, GPR126, PS1TP2

Entrez Gene IDs

57211 (Human); 215798 (Mouse); 308376 (Rat)

Gene Symbol

ADGRG6

UniProt

Product Documents for Recombinant Human VIGR/GPR126 Fc Chimera Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human VIGR/GPR126 Fc Chimera Protein, CF

For research use only

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