Human embryonic kidney cell, HEK293-derived human Vasorin/SLIT-like 2 protein Cys24-Asn573, with a C-terminal 6-His tag
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
83-95 kDa, under reducing conditions
Measured by its binding ability in a functional ELISA. When Recombinant Human TGF‑ beta 1 (Catalog # 240-B) is used at 2 µg/mL (100 µL/well), Recombinant Human Vasorin/SLIT‑like 2 (Catalog # 10156-VN) binds with an ED50 of 0.6-3.6 μg/mL.
Recombinant Human Vasorin/SLIT-like 2 His-tag Protein, CF Scientific Data Examples
Recombinant Human Vasorin/SLIT-like 2 His-tag Protein Binding Activity
When Recombinant Human TGF- beta 1 (Catalog # 240-B) is used at 2 µg/mL (100 µL/well), Recombinant Human Vasorin/SLIT-like 2 (Catalog # 10156-VN) binds with an ED50 of 0.6-3.6 µg/mL.
Recombinant Human Vasorin/SLIT-like 2 His-tag Protein SDS-PAGE
2 μg/lane of Recombinant Human Vasorin/SLIT-like 2 His-tag (Catalog # 10156-VN) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 83-95 kDa.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitute at 500 μg/mL in PBS.
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Vasorin/SLIT-like 2
Vasorin, also known as SLIT-like 2, is an approximately 110-kDa transmembrane protein that is expressed on vascular smooth muscle cells and in the developing skeletal system (1, 2). It is down-regulated following vascular injury (1). A 95-kDa soluble form of Vasorin can be shed from the cell surface by ADAM17-mediated cleavage (3). The shed soluble form of Vasorin binds to TGF-beta 1, 2, and 3 and prevents TGF-beta-induced effects including intimal formation and epithelial mesenchymal transition (1, 3). Soluble Vasorin is elevated in the serum of hepatic carcinoma patients relative to normal or non-cancerous hepatic disease (4). It promotes tumor cell proliferation, migration, and survival (4). Mature human Vasorin consists of a 552 amino acid (aa) extracellular domain (ECD) with 10 leucine rich repeats (LRR) flanked by one LRRNT and one LRRCT domain, one EGF-like domain, and one Fibronectin type III domain, a 21 aa transmembrane segment, and a 77 aa cytoplasmic domain (1). Within the ECD, human Vasorin shares 85% and 86% aa sequence identity with mouse and rat Vasorin, respectively.
Ikeda, Y. et al. (2004) Proc. Natl. Acad. Sci. USA 101:10732.
Krautzberger, A.M. et al. (2012) Gene Expr. Patterns 12:167.
Malapeira, J. et al. (2011) Oncogene 30:1912.
Li, S. et al. (2015) Oncotarget 6:10045.
Entrez Gene IDs
114990 (Human); 246154 (Mouse)
Atia, Protein slit-like 2, SLIT like 2, Slitl2, VASN, Vasorin, slit-like 2, slit-like 2 (Drosophila)
Currently there are no citations for Recombinant Human Vasorin/SLIT-like 2 His-tag Protein, CF.
Which Brands are Currently Available on bio-techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments and Simple Plex Assays are available to quote. ProteinSimple branded instrument consumables and ACD branded products will be available on bio-techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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