Recombinant Human TRAIL R2 Fc Alexa Fluor® 488 Protein
R&D Systems, part of Bio-Techne | Catalog # AFG10140
Fc Chimera
Key Product Details
- R&D Systems CHO-derived Recombinant Human TRAIL R2 Fc Alexa Fluor® 488 Protein (AFG10140)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
CHO
Accession #
Structure / Form
Disulfide-linked homodimer
Labeled with Alexa Fluor® 488 via amines
Excitation Wavelength: 488 nm
Emission Wavelength: 515-545 nm
Labeled with Alexa Fluor® 488 via amines
Excitation Wavelength: 488 nm
Emission Wavelength: 515-545 nm
Conjugate
Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)
Applications
Bioactivity
Product Specifications
Source
Chinese Hamster Ovary cell line, CHO-derived human TRAIL R2/TNFRSF10B protein
| Human TRAIL R2/TNFRSF10B (Ala54-Ser210) Accession # AAH01281.1 |
IEGRMD | Human IgG1 (Pro100-Lys330) |
| N-terminus | C-terminus |
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ala54
Predicted Molecular Mass
44 kDa
SDS-PAGE
54-61 kDa, under reducing conditions.
Activity
Measured by flow cytometry for its ability to bind anti-human TRAILR2 Antibody conjugated beads. The concentration of Recombinant Human TRAIL R2/TNFRSF10B Fc Chimera Alexa Fluor® 488 (Catalog # AFG10140) that produces 50% of the binding response is 6.00-60.0 ng/mL.
Scientific Data Images for Recombinant Human TRAIL R2 Fc Alexa Fluor® 488 Protein
Flow cytometry analysis for Recombinant Human TRAIL R2/TNFRSF10B Fc Chimera Alexa Fluor® 488 staining on anti-human TRAILR2 Antibody conjugated beads.
Streptavidin coated beads conjugated to biotinylated anti-human TRAILR2 Antibody were stained with the indicated concentrations of Recombinant Human TRAIL R2/TNFRSF10B Fc Chimera Alexa Fluor® 488 (Catalog # AFG10140).
Recombinant Human TRAIL R2/TNFRSF10B Fc Chimera Alexa Fluor® 488 Protein SDS-PAGE.
2 μg/lane of Recombinant Human TRAIL R2/TNFRSF10B Fc Chimera Alexa Fluor® 488 Protein (Catalog # AFG10140) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 54-61 kDa and 110-120 kDa, respectively.Formulation, Preparation and Storage
AFG10140
| Formulation | Supplied as a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: TRAIL R2/TNFRSF10B
References
- Chaudhary, P.M. et al. (1997) Immunity 7:821.
- Walczak, H. et al. (1997) EMBO J. 16:5386.
- Golstein, P. (1997) Curr. Biol. 7:R750.
Long Name
TRAIL Receptor 2
Alternate Names
CD262, DR5, TNFRSF10B, TRAILR2
Gene Symbol
TNFRSF10B
UniProt
Additional TRAIL R2/TNFRSF10B Products
Product Documents for Recombinant Human TRAIL R2 Fc Alexa Fluor® 488 Protein
Product Specific Notices for Recombinant Human TRAIL R2 Fc Alexa Fluor® 488 Protein
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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