Recombinant Human TNF-alpha (HEK293-expressed) Protein, CF

Name: Recombinant Human TNF-alpha (HEK293-expressed) Protein, CF
Brand: R&D Systems
SKU: 10291-TA
Rating: 4 (3 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 10291-TA

Citations (1)

(3)

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Analyzed by SEC-MALS

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Key Product Details

Source:	HEK293
Accession #:	P01375
Applications:	Bioactivity

View Full Technical Details for Recombinant Human TNF-alpha (HEK293-expressed) Protein, CF

Product Specifications for Recombinant Human TNF-alpha (HEK293-expressed) Protein, CF

Source	Human embryonic kidney cell, HEK293-derived human TNF-alpha protein Val77-Leu233
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Val77
Predicted Molecular Mass	17 kDa
SDS-PAGE	14-18 kDa, under reducing conditions
Activity	Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The ED₅₀ for this effect is 20-100 pg/mL.

Scientific Data Examples for Recombinant Human TNF-alpha (HEK293-expressed) Protein, CF

	Recombinant Human TNF-alpha (HEK293-expressed) Protein SEC-MALS Recombinant human TNF-alpha (Catalog # 10291-TA) has a molecular weight (MW) of 52.3 kDa as analyzed by SEC-MALS, suggesting that this protein is a homotrimer. MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).
	Recombinant Human TNF-alpha (HEK293-expressed) Protein Bioactivity Recombinant Human TNF‑ alpha (HEK293-expressed) (Catalog # 10291-TA) induces cytotoxicity in the L‑929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED₅₀ for this effect is 20‑100 pg/mL.
	Recombinant Human TNF-alpha (HEK293-expressed) Protein SDS-PAGE 2 μg/lane of Recombinant Human TNF-alpha (HEK293-expressed) (Catalog # 10291-TA) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 14-18 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10291-TA

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: TNF-alpha

Tumor necrosis factor alpha (TNF-alpha ), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism (1, 2). Human TNF-alpha consisits of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 177 aa extracellular domain (ECD) (3). Within the ECD, human TNF-alpha shares 97% aa sequence identity with rhesus and 71%-92% with bovine, canine, cotton rat, equine, feline, mouse, porcine, and rat TNF-alpha. TNF-alpha is produced by a wide variety of immune, epithelial, endothelial, and tumor cells (1, 2). TNF-alpha is assembled intracellularly to form a noncovalently linked homotrimer which is expressed on the cell surface (4). Cell surface TNF-alpha can induce the lysis of neighboring tumor cells and virus infected cells, and it can generate its own downstream cell signaling following ligation by soluble TNFR I (2, 5). Shedding of membrane bound TNF-alpha by TACE/ADAM17 releases the bioactive cytokine, a 55 kDa soluble trimer of the TNF-alpha extracellular domain (6-8). TNF-alpha binds the ubiquitous 55-60 kDa TNF RI (9, 10) and the hematopoietic cell-restricted 80 kDa TNF RII (11, 12), both of which are also expressed as homotrimers (1, 2, 13). Both type I and type II receptors bind TNF-alpha with comparable affinity (14), although only TNF RI contains a cytoplasmic death domain which triggers the activation of apoptosis. Soluble forms of both types of receptors are released and can neutralize the biological activity of TNF-alpha (15).

Item	Value
References	Zelova, H. and J. Hosek (2013) Inflamm. Res. 62:641. Juhasz, K. et al. (2013) Expert Rev. Clin. Immunol. 9:335. Pennica, D. et al. (1984) Nature 312:724. Tang, P. et al. (1996) Biochemistry 35:8216. Perez, C. et al. (1990) Cell 63:251. Black, R.A. et al. (1997) Nature 385:729. Moss, M.L. et al. (1997) Nature 385:733. Gearing, A.J.H. et al. (1994) Nature 370:555. Schall, T.J. et al. (1990) Cell 61:361. Loetscher, H. et al. (1990) Cell 61:351. Dembic, Z. et al. (1990) Cytokine 2:231. Smith, C.A. et al. (1990) Science 248:1019. Loetscher, H. et al. (1991) J. Biol. Chem. 266:18324. Pinckard, J.K. et al. (1997) J. Biol. Chem. 272:10784. Engelmann, H. et al. (1990) J. Biol. Chem. 265:1531.
Long Name	Tumor Necrosis Factor alpha
Entrez Gene IDs	7124 (Human); 21926 (Mouse); 24835 (Rat); 397086 (Porcine); 280943 (Bovine); 403922 (Canine); 102139631 (Cynomolgus Monkey); 100033834 (Equine); 493755 (Feline); 100009088 (Rabbit)
Alternate Names	APC1 protein, Cachectin, Cachetin, DIF, TNF, TNF, monocyte-derived, TNF-A, TNF-alpha, TNF-alphacachectin, TNFA, TNFATNF, macrophage-derived, TNFG1F, TNFSF1A, TNFSF2, TNFSF2TNF superfamily, member 2, TNFalpha, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, tumor necrosis factor-alpha

Citations for Recombinant Human TNF-alpha (HEK293-expressed) Protein, CF (1)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human TNF-alpha (HEK293-expressed) Protein, CF include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

S Salva et al. (2021-01-01), Lactobacillus rhamnosus postbiotic-induced immunomodulation as safer alternative to the use of live bacteria Cytokine, 146(0):155631.
PMID: 34252871

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Customer Reviews for Recombinant Human TNF-alpha (HEK293-expressed) Protein, CF (3)

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Recombinant Human TNF-alpha (HEK293-expressed) Protein, CF

Name: Anonymous

Verified Customer | Posted 06/15/2022
Recombinant Human TNF-alpha (HEK293-expressed) Protein, CF

Name: Anonymous

Application: In vitro bioactivity in cell culture

Verified Customer | Posted 11/18/2020
Recombinant Human TNF-alpha (HEK293-expressed) Protein, CF

Name: Jayalakshmi Miriyala

Application: Stem/Immune cell maintenance or differentiation

Reason for Rating: I used this for the diffrentiation of monocytes to dendritic cell and it worked very well

Verified Customer | Posted 10/01/2020

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Recombinant Human TNF-alpha (HEK293-expressed) Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Human TNF-alpha (HEK293-expressed) Protein, CF

Scientific Data Examples for Recombinant Human TNF-alpha (HEK293-expressed) Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: TNF-alpha

Citations for Recombinant Human TNF-alpha (HEK293-expressed) Protein, CF (1)

Customer Reviews for Recombinant Human TNF-alpha (HEK293-expressed) Protein, CF (3)

Customer Images

Recombinant Human TNF-alpha (HEK293-expressed) Protein, CF

Recombinant Human TNF-alpha (HEK293-expressed) Protein, CF

Recombinant Human TNF-alpha (HEK293-expressed) Protein, CF

Product Documents for Recombinant Human TNF-alpha (HEK293-expressed) Protein, CF

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