Recombinant Human TNF-alpha, Animal-Free Protein
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Customer Reviews for Recombinant Human TNF-alpha, Animal-Free Protein (1)
Application: In vitro bioactivity in cell cultureReason for Rating: TNF-a protein was used successfully as a control of mice-derived pre-adipocytes activation during their maturation to adipocytes.Verified Customer | Posted 10/22/2021C57BL/6J-derived pre-adipocytes were extracted from adipose tissue. After culturing for 3 weeks, cells were stimulated with TNF-alpha as pro-inflammatory control, while the other cells were treated with a Moringa oleifera extract (10, 50, and 100 micrograms/mL). The quantification of Reactive oxygen species in the cells showed an adequate impact of TNF-alpha inducing inflammation and ROS activation. ROS were measured by fluorescence in relative fluorescence units (RFU).
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