Recombinant Human TIM-1/KIM-1/HAVCR (275 aa) Fc Protein, CF New

Name: Recombinant Human TIM-1/KIM-1/HAVCR (275 aa) Fc Protein, CF
Brand: R&D Systems
SKU: 11273-TM

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 11273-TM

Product Datasheet / COA / SDS

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Setting the standard in quality research reagents

Key Product Details

Source:	HEK293
Accession #:	Q96D42.3
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Human TIM-1/KIM-1/HAVCR (275 aa) Fc Protein, CF

Product Specifications for Recombinant Human TIM-1/KIM-1/HAVCR (275 aa) Fc Protein, CF

Source

Human embryonic kidney cell, HEK293-derived human TIM-1/KIM-1/HAVCR protein

Human TIM-1/KIM-1/HAVCR (Ser21-Gly295) Accession # Q96D42.3	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Ser21

Predicted Molecular Mass

56 kDa

SDS-PAGE

113-132 kDa, under reducing conditions.

Activity

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells. The ED₅₀ for this effect is 0.500-10.0 μg/mL.

Scientific Data Examples for Recombinant Human TIM-1/KIM-1/HAVCR (275 aa) Fc Protein, CF

	Recombinant Human TIM-1/KIM-1/HAVCR (175 aa) Fc Chimera Protein Bioactivity. Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells. The ED₅₀ for this effect is 0.500-10.0 μg/mL.
	Recombinant Human TIM-1/KIM-1/HAVCR (175 aa) Fc Chimera Protein SDS-PAGE. 2 μg/lane of Recombinant Human TIM-1/KIM-1/HAVCR (175 aa) Fc Chimera Protein (Catalog # 11273-TM) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 113-132 kDa and 230-260 kDa, respectively.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11273-TM

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background:

T cell-immunoglobulin and mucin domain 1 (TIM-1), also known as KIM1 and HAVcr1, type I transmembrane glycoprotein in the TIM subfamily of immunoglobulin (Ig) superfamily molecules. In human, the TIM family is comprised of 3 family members and is involved in the regulation of Th1 and Th2‑cell‑mediated immune responses (1). Mature TIM-1 consists of an extracellular domain (ECD) with one V‑type Ig‑like domain and a mucin domain with O- and N-linked carbohydrates, a transmembrane segment, and a cytoplasmic signaling domain. Within the ECD, human TIM-1 shares 39% amino acid sequence identity with mouse TIM-1. Multiple TIM-1 variants can be produced either through polymorphisms or alternative splicing primarily resulting in deletions in the mucin domain (2). A soluble form of TIM-1, detectable in the urine and in circulation, can be generated through ectodomain cleavage mediated by MMP3 (3, 4). TIM-1 is expressed on splenic B cells, IL-10+ regulatory B cells, CD4+ T cells, mast cells, dendritic cells, kidney epithelium and a broad range of mucosal epithelium (5-7). It is upregulated on activated Th2 cells, after dendritic cell maturation, and on kidney tubular epithelial cells after injury (8-10). TIM-1 ligation induces T cell proliferation and promotes cytokine production (5, 11). TIM-1 serves as a receptor for phosphatidylserine, and its interaction with LMIR5 enables TIM-1 to mediate the phagocytosis of apoptotic cells (12). TIM-1 also serves as a cellular entry receptor for various viruses, including hepatitis A virus, Ebolavirus, Marburgvirus and has been indicated as a possible receptor for SARS-CoV-2 (13-15).

References

Du, P. et al. (2016) J. Immunol. Res. 2016:8605134.
Freeman, G.J. et al. (2010) Immunol. Rev. 235:172.
Lim, A.I. et al. (2012) Int. J. Biochem. Cell Biol. 44:1040.
Bailly, V. et al. (2002) J. Biol. Chem. 277:39739.
Ding, Q. et al. (2011) J. Clin. Invest. 121:3645.
Ma, J. et al. (2011) Biochem. Biophys. Res. Commun. 406:223.
de Souza, A.J. et al. (2005) Proc. Natl. Acad. Sci. USA 102:17113.
Kuehn, E.W. et al. (2002) Am. J. Physiol. Renal Physiol. 283:F1326.
Umetsu, S.E. et al. (2005) Nat. Immunol. 6:447.
Xiao, S. et al. (2011) Eur. J. Immunol. 41:1539.
Meyers, J.H. et al. (2005) Nat. Immunol. 6:455.
Kobayashi, N. et al. (2007) Immunity 27:927.
Tami, C. et al. (2007) J. Virol. 81:3437.
Kondratowicz, A.S. et al. (2011) Proc. Natl. Acad. Sci. USA 108:8426.
Ichimura, T. et al. (2020) medRxiv https://doi.org/10.1101/2020.09.16.20190694.

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Recombinant Human TIM-1/KIM-1/HAVCR (275 aa) Fc Protein, CF New

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Product Specifications for Recombinant Human TIM-1/KIM-1/HAVCR (275 aa) Fc Protein, CF

Scientific Data Examples for Recombinant Human TIM-1/KIM-1/HAVCR (275 aa) Fc Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background:

Citations for Recombinant Human TIM-1/KIM-1/HAVCR (275 aa) Fc Protein, CF

Product Documents for Recombinant Human TIM-1/KIM-1/HAVCR (275 aa) Fc Protein, CF

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