Recombinant Human TIGIT (T103) His-tag Avi-tag Protein, CF

Name: Recombinant Human TIGIT (T103) His-tag Avi-tag Protein, CF
Brand: R&D Systems
SKU: AVI11124
Price: 487 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AVI11124

Product Datasheet / COA / SDS

Biotinylated

Catalog #

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Qty

Setting the standard in quality research reagents

Key Product Details

Source:	CHO
Accession #:	Q495A1.1
Structure / Form:	Biotinylated via Avi-tag
Applications:	Bioactivity

View Full Technical Details for Recombinant Human TIGIT (T103) His-tag Avi-tag Protein, CF

Product Specifications for Recombinant Human TIGIT (T103) His-tag Avi-tag Protein, CF

Source

Chinese Hamster Ovary cell line, CHO-derived human TIGIT protein

Human TIGIT (Met22-Gln139, T103) Accession # Q495A1.1	6-His tag	Avi-tag
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Met22

Predicted Molecular Mass

21 kDa

SDS-PAGE

18-29 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human CD155/PVR His Protein (Catalog # 2530-CD) is immobilized at 10.0 µg/mL (100 µL/well), the concentration of Biotinylated Recombinant Human TIGIT His Chimera Avi-tag (Catalog # AVI11124) that produces 50% of the optimal binding response is 0.500-5.00 μg/mL.

Scientific Data Examples for Recombinant Human TIGIT (T103) His-tag Avi-tag Protein, CF

	Biotinylated Recombinant Human TIGIT (T103) His-tag Avi-tag Protein Binding Activity When Recombinant Human CD155/PVR His Protein (2530-CD) is immobilized at 10.0 µg/mL (100 µL/well), the concentration of Biotinylated Recombinant Human TIGIT His Chimera Avi-tag Protein (Catalog # AVI11124) that produces 50% of the optimal binding response is 0.500-5.00 μg/mL.
	Biotinylated Recombinant Human TIGIT (T103) His-tag Avi-tag Protein SDS-PAGE 2 μg/lane of Biotinylated Recombinant Human TIGIT (T103) His-tag Avi-tag Protein (Catalog # AVI11124) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 18-29 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI11124

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 200 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TIGIT

TIGIT (T cell Immunoreceptor with Ig and ITIM domains), also called Vstm3 (V-set and transmembrane domain-containing 3), Vsig9 (V-set and Ig domain-containing 9) and WUCAM (Washington University cell adhesion molecule) is a 30-34 kDa type I transmembrane protein that is a member of the CD28 family within the Ig superfamily of proteins (1-4). Human TIGIT cDNA encodes 244 amino acids (aa) including a 21 aa signal sequence, a 120 aa extracellular region with a V-type Ig-like domain and two potential N-glycosylation site, a 21 aa transmembrane sequence, and an 82 aa cytoplasmic domain with an ITIM motif (5). Within the ECD, human TIGIT shares only 65% and 67% aa sequence identity with mouse and rat TIGIT. TIGIT is expressed on NK cells and subsets of activated, memory, and regulatory T cells, and particularly on follicular helper T cells within secondary lymphoid organs (1, 2, 6-8). It binds to CD155/PVR/Necl-5 and Nectin-2/CD112/PVRL2 that appear on dendritic cells (DC) and endothelium (1-3, 7). Binding of TIGIT by DC induces IL-10 release and inhibits IL-12 production (2). Ligation of TIGIT on T cells down‑regulates TCR-mediated activation and subsequent proliferation, while in NK cell, TIGIT ligation blocks NK cell cytotoxicity (6-8). Through CD155 and Nectin-2, which also interact with DNAM-1/CD226 and CD96/Tactile, TIGIT is part of an interacting network of Ig superfamily members that may augment or oppose each other (3, 4, 6, 7). In particular, TIGIT binding to CD155 can antagonize the effects of DNAM-1 (6, 7). Soluble TIGIT is able to compete with DNAM-1 for CD155 binding and attenuates T cell responses, while mice lacking TIGIT show increased T cell responses and susceptibility to autoimmune challenges (2, 3, 8). Our Avi-tag Biotinylated Human TIGIT (T103) His-tag protein features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

Item	Value
References	Boles, K.S. et al. (2009) Eur. J. Immunol. 39:695. Yu, X. et al. (2009) Nat. Immunol. 10:48. Levin, S.D. et al. (2011) Eur. J. Immunol. 41:902. Xu, Z. et al. (2010) Cell. Mol. Immunol. 7:11. SwissProt Accession # Q495A1. Seth, S. et al. (2009) Eur. J. Immunol. 39:3160. Stanietsky, N. et al. (2009) Proc. Natl. Acad. Sci. USA 106:17858. Joller, N. et al. (2011) J. Immunol. 83:1338.
Long Name	T Cell Immunoreceptor with Ig and ITIM Domains
Entrez Gene IDs	201633 (Human); 100043314 (Mouse); 363784 (Rat); 102121588 (Cynomolgus Monkey)
Alternate Names	T cell immunoreceptor with Ig and ITIM domains, TIGIT, VSIG9, VSTM3, WUCAM

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Recombinant Human TIGIT (T103) His-tag Avi-tag Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Human TIGIT (T103) His-tag Avi-tag Protein, CF

Scientific Data Examples for Recombinant Human TIGIT (T103) His-tag Avi-tag Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: TIGIT

Citations for Recombinant Human TIGIT (T103) His-tag Avi-tag Protein, CF

Product Documents for Recombinant Human TIGIT (T103) His-tag Avi-tag Protein, CF

Product Datasheets

COA

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