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Recombinant Human TIGIT (T103) Fc Chimera Protein, CF

Bio-Techne includes R&D Systems | Catalog # 9464-TG

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9464-TG-050

Key Product Details

Source

HEK293

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human TIGIT protein
Human TIGIT
(Met22-Pro141, T103)
Accession # Q495A1-1
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met22

Predicted Molecular Mass

40 kDa

SDS-PAGE

44-60 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human CD155/PVR (Catalog # 2530‑CD) is coated at 2.5 μg/mL (100 μL/well), the concentration of Recombinant Human TIGIT (T103) Fc Chimera that produces 50% optimal binding response is typically 8-48 ng/mL.

Scientific Data Images for Recombinant Human TIGIT (T103) Fc Chimera Protein, CF

Recombinant Human TIGIT (T103) Fc Chimera Protein Bioactivity

Recombinant Human TIGIT (T103) Fc Chimera Protein Bioactivity

When Recombinant Human CD155/PVR (Catalog # 2530-CD) is coated at 2.5 µg/mL (100 µL/well), Recombinant Human TIGIT (T103) Fc Chimera (Catalog # 9464-TG) binds with a typical ED50 of 8-48 ng/mL.

Formulation, Preparation and Storage

9464-TG
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -70 °C under sterile conditions after reconstitution.

Background: TIGIT

TIGIT (T cell Immunoreceptor with Ig and ITIM domains), also called Vstm3 (V-set and transmembrane domain-containing 3), Vsig9 (V-set and Ig domain-containing 9) and WUCAM (Washington University cell adhesion molecule) is a 30-34 kDa type I transmembrane protein that is a member of the CD28 family within the Ig superfamily of proteins (1-4). Human TIGIT cDNA encodes 244 amino acids (aa) including a 21 aa signal sequence, a 120 aa extracellular region with a V-type Ig-like domain and two potential N-glycosylation site, a 21 aa transmembrane sequence, and an 82 aa cytoplasmic domain with an ITIM motif (5). A 170 aa variant diverges after aa 166 (5). Within the ECD, human TIGIT shares only 68-75% aa sequence identity with mouse, porcine, canine, equine and bovine TIGIT (1, 2). Binding of TIGIT by DC induces IL-10 release and inhibits IL-12 production (2). Ligation of TIGIT on T cells down‑regulates TCR-mediated activation and subsequent proliferation, while NK cell TIGIT ligation blocks NK cell cytotoxicity (6-8). Through CD155 and Nectin-2, which also interact with DNAM-1/CD226 and CD96/Tactile, TIGIT is part of an interacting network of Ig superfamily members that may augment or oppose each other (3, 4, 6, 7). In particular, TIGIT binding to CD155 can antagonize the effects of DNAM-1 (6, 7). Soluble TIGIT is able to compete with DNAM-1 for CD155 binding and attenuates T cell responses, while mice lacking TIGIT show increased T cell responses and susceptibility to autoimmune challenges (2, 3, 8).

References

  1. Boles, K.S. et al. (2009) Eur. J. Immunol. 39:695.
  2. Yu, X. et al. (2009) Nat. Immunol. 10:48.
  3. Levin, S.D. et al. (2011) Eur. J. Immunol. 41:902
  4. Xu, Z. et al. (2010) Cell. Mol. Immunol. 7:11.
  5. SwissProt Accession # Q495A1.
  6. Seth, S. et al. (2009) Eur. J. Immunol. 39:3160.
  7. Stanietsky, N. et al. (2009) Proc. Natl. Acad. Sci. USA 106:17858.
  8. Joller, N. et al. (2011) J. Immunol. 83:1338.

Long Name

T Cell Immunoreceptor with Ig and ITIM Domains

Alternate Names

VSIG9, VSTM3, WUCAM

Entrez Gene IDs

201633 (Human); 100043314 (Mouse); 363784 (Rat); 102121588 (Cynomolgus Monkey)

Gene Symbol

TIGIT

UniProt

Additional TIGIT Products

Product Documents for Recombinant Human TIGIT (T103) Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human TIGIT (T103) Fc Chimera Protein, CF

For research use only

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