Recombinant Human TIGIT (T103) Fc Chimera Protein, CF

Name: Recombinant Human TIGIT (T103) Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 9464-TG
Rating: 5 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 9464-TG

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Key Product Details

Source:	HEK293
Accession #:	Q495A1-1
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

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Product Specifications for Recombinant Human TIGIT (T103) Fc Chimera Protein, CF

Source

Human embryonic kidney cell, HEK293-derived human TIGIT protein

Human TIGIT (Met22-Pro141, T103) Accession # Q495A1-1	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Met22

Predicted Molecular Mass

40 kDa

SDS-PAGE

44-60 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human CD155/PVR (Catalog # 2530‑CD) is coated at 2.5 μg/mL (100 μL/well), the concentration of Recombinant Human TIGIT (T103) Fc Chimera that produces 50% optimal binding response is typically 8-48 ng/mL.

Scientific Data Examples for Recombinant Human TIGIT (T103) Fc Chimera Protein, CF

	Recombinant Human TIGIT (T103) Fc Chimera Protein Bioactivity When Recombinant Human CD155/PVR (Catalog # 2530-CD) is coated at 2.5 µg/mL (100 µL/well), Recombinant Human TIGIT (T103) Fc Chimera (Catalog # 9464-TG) binds with a typical ED₅₀ of 8-48 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9464-TG

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TIGIT

TIGIT (T cell Immunoreceptor with Ig and ITIM domains), also called Vstm3 (V-set and transmembrane domain-containing 3), Vsig9 (V-set and Ig domain-containing 9) and WUCAM (Washington University cell adhesion molecule) is a 30-34 kDa type I transmembrane protein that is a member of the CD28 family within the Ig superfamily of proteins (1-4). Human TIGIT cDNA encodes 244 amino acids (aa) including a 21 aa signal sequence, a 120 aa extracellular region with a V-type Ig-like domain and two potential N-glycosylation site, a 21 aa transmembrane sequence, and an 82 aa cytoplasmic domain with an ITIM motif (5). A 170 aa variant diverges after aa 166 (5). Within the ECD, human TIGIT shares only 68-75% aa sequence identity with mouse, porcine, canine, equine and bovine TIGIT (1, 2). Binding of TIGIT by DC induces IL-10 release and inhibits IL-12 production (2). Ligation of TIGIT on T cells down‑regulates TCR-mediated activation and subsequent proliferation, while NK cell TIGIT ligation blocks NK cell cytotoxicity (6-8). Through CD155 and Nectin-2, which also interact with DNAM-1/CD226 and CD96/Tactile, TIGIT is part of an interacting network of Ig superfamily members that may augment or oppose each other (3, 4, 6, 7). In particular, TIGIT binding to CD155 can antagonize the effects of DNAM-1 (6, 7). Soluble TIGIT is able to compete with DNAM-1 for CD155 binding and attenuates T cell responses, while mice lacking TIGIT show increased T cell responses and susceptibility to autoimmune challenges (2, 3, 8).

Item	Value
References	Boles, K.S. et al. (2009) Eur. J. Immunol. 39:695. Yu, X. et al. (2009) Nat. Immunol. 10:48. Levin, S.D. et al. (2011) Eur. J. Immunol. 41:902 Xu, Z. et al. (2010) Cell. Mol. Immunol. 7:11. SwissProt Accession # Q495A1. Seth, S. et al. (2009) Eur. J. Immunol. 39:3160. Stanietsky, N. et al. (2009) Proc. Natl. Acad. Sci. USA 106:17858. Joller, N. et al. (2011) J. Immunol. 83:1338.
Long Name	T Cell Immunoreceptor with Ig and ITIM Domains
Entrez Gene IDs	201633 (Human); 100043314 (Mouse); 363784 (Rat); 102121588 (Cynomolgus Monkey)
Alternate Names	T cell immunoreceptor with Ig and ITIM domains, TIGIT, VSIG9, VSTM3, WUCAM

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Customer Reviews for Recombinant Human TIGIT (T103) Fc Chimera Protein, CF (1)

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Recombinant Human TIGIT (T103) Fc Chimera Protein, CF

Name: Jenna Nguyen

Application: Binding assay/Protein-protein interaction

Verified Customer | Posted 07/27/2018

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Product Documents for Recombinant Human TIGIT (T103) Fc Chimera Protein, CF

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Recombinant Human TIGIT (T103) Fc Chimera Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Complete Your Research

Complete Your Experiment

Product Specifications for Recombinant Human TIGIT (T103) Fc Chimera Protein, CF

Scientific Data Examples for Recombinant Human TIGIT (T103) Fc Chimera Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: TIGIT

Citations for Recombinant Human TIGIT (T103) Fc Chimera Protein, CF

Customer Reviews for Recombinant Human TIGIT (T103) Fc Chimera Protein, CF (1)

Recombinant Human TIGIT (T103) Fc Chimera Protein, CF

Product Documents for Recombinant Human TIGIT (T103) Fc Chimera Protein, CF

Product Datasheets

COA

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SDS

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