Recombinant Human Sortilin Protein, CF

Name: Recombinant Human Sortilin Protein, CF
Brand: R&D Systems
SKU: 3154-ST
Rating: 5 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 3154-ST

Citations (2)

(1)

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Key Product Details

Source:	NS0
Accession #:	Q99523
Structure / Form:	Monomer
Applications:	Binding Activity

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Product Specifications for Recombinant Human Sortilin Protein, CF

Source	Mouse myeloma cell line, NS0-derived human Sortilin protein Ser78-Asn755, with a C-terminal 6-His tag
Purity	>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ser78
Predicted Molecular Mass	76.6 kDa
SDS-PAGE	95-100 kDa, reducing conditions
Activity	Measured by its binding ability in a functional ELISA. Immobilized rhSortilin at 4 µg/mL (100 µL/well) can bind rh beta -NGF with a linear range of 3-200 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3154-ST

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Sortilin

Sortilin (neurotensin receptor 3, glycoprotein 95) is a 95 kDa Type I transmembrane monomeric glycoprotein that is one of five known members of the mammalian vacuolar protein sorting 10p domain (Vps10p-D) family of sorting receptors (1, 2). Human preprosortilin is processed by signal sequence cleavage followed by propeptide cleavage at a furin recognition site. The cationic propeptide exhibits pH-dependent high affinity binding that blocks the Sortilin ligand binding site both pre- and post-cleavage (3). The extracellular/luminal sequence comprises the Vps10p domain, including 10 conserved cysteines (10 CC) essential for ligand binding (2). The cytoplasmic domain sorting motifs confer all trafficking during synthesis, targeting to lysosomes, endocytosis and Golgi-endosome transport; as little as 10% may be found on the cell surface (4). Mature human Sortilin shares 91% aa identity with mouse and rat Sortilin and 93% aa identity with dog. During murine development, sortilin is mainly expressed in the nervous system (5) where it is a receptor for neuropeptides including neurotensin, nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) (6 - 9). ProNGF (or the NGF propeptide alone) binds sortilin with much higher affinity (Kd ~5 - 8 nM) than does mature NGF (Kd ~90 nM). The complex of sortilin, pro-NGF and the receptor p75^ntr results in endocytosis of proNGF and induction of apoptosis (7). Similar results have been obtained with proBDNF and BDNF (8, 9). Sortilin is expressed in other tissues including testis, skeletal muscle and fat (1, 10). It is essential and sufficient for biogenesis of Glut4 storage vesicles necessary for insulin responsiveness in adipocytes (10). Sortilin also binds lipoprotein lipase (11), apoE (2) and RAP (1, 11). Binding is competitive, indicating that although unrelated, targets likely bind the same site.

Item	Value
References	Petersen, C.M. et al. (1997) J. Biol. Chem. 272:3599. Westergaard, U.B. et al. (2004) J. Biol. Chem. 279:50221. Petersen, C.M. et al. (1998) EMBO J. 18:595. Nielsen, M.S. et al. (2001) EMBO J. 20:2180. Hermans-Borgmeyer, I. et al. (1999) Mol. Brain Res. 65:216. Mazella, J. et al. (1998) J. Biol. Chem. 273:26273. Nykjaer, A et al. (2004) Nature 427:843. Teng, H.K. et al. (2005) J. Neurosci. 25:5455. Chen, Z-Y et al. (2004) J. Neurosci. 25:6156. Shi, J and K.V. Kandror (2005) Dev. Cell 9:99. Nielsen, M. S. et al. (1999) J. Biol. Chem. 274:8832.
Entrez Gene IDs	6272 (Human); 20661 (Mouse)
Alternate Names	100 kDa NT receptor, Glycoprotein 95, Gp95, Gp95LDLCQ6, NT3NTR3, Neurotensin receptor 3, Ntr3, SORT1, Sortilin, sortilin 1

Citations for Recombinant Human Sortilin Protein, CF (2)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human Sortilin Protein, CF include:

Species: Human
Sample Types: Recombinant Protein
Applications: Octet Biosensor

S Miyakawa et al. (2020), Anti-sortilin1 Antibody Up-Regulates Progranulin via Sortilin1 Down-Regulation Frontiers in Neuroscience, 14(0):586107.
PMID: 33384578
Species: N/A
Sample Types: N/A
Applications: Antibody Absorption

Fauchais AL et al. (2008), Role of endogenous brain-derived neurotrophic factor and sortilin in B cell survival. J. Immunol., 181(5):3027-38.
PMID: 18713973

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Recombinant Human Sortilin Protein, CF

Name: Anonymous

Application: Immunoassay Standard

Reason for Rating: Works great for ELISA assay

Verified Customer | Posted 10/13/2021

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Product Documents for Recombinant Human Sortilin Protein, CF

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Recombinant Human Sortilin Protein, CF

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Product Specifications for Recombinant Human Sortilin Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: Sortilin

Citations for Recombinant Human Sortilin Protein, CF (2)

Customer Reviews for Recombinant Human Sortilin Protein, CF (1)

Customer Images

Recombinant Human Sortilin Protein, CF

Product Documents for Recombinant Human Sortilin Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

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