Recombinant Human SorLA His-tag Protein, CF

Name: Recombinant Human SorLA His-tag Protein, CF
Brand: R&D Systems
SKU: 11083-LA
Price: 581 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 11083-LA

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Setting the standard in quality research reagents

Key Product Details

Source:	CHO
Accession #:	NP_003096.1
Applications:	Bioactivity

View Full Technical Details for Recombinant Human SorLA His-tag Protein, CF

Product Specifications for Recombinant Human SorLA His-tag Protein, CF

Source	Chinese Hamster Ovary cell line, CHO-derived human SorLA protein Ser82-Asp2135, with a C-terminal 6-His tag
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ser82
Predicted Molecular Mass	232 kDa
SDS-PAGE	270-345 kDa, under reducing conditions.
Activity	Measured by its binding ability in a functional ELISA. When Recombinant Human SorLA His-tag Protein is coated at 0.250 μg/mL (100 μL/well), it binds to Recombinant Human PDGF-BB (Catalog # 220-BB). The ED₅₀ for this binding is 2.00-30.0 ng/mL.

Scientific Data Examples for Recombinant Human SorLA His-tag Protein, CF

	Recombinant Human SorLA His-tag Protein Binding Activity. When Recombinant Human SorLA His-tag Protein (Catalog # 11083-LA) is coated at 0.250 μg/mL (100 μL/well), it binds to Recombinant Human PDGF-BB (220-BB). The ED₅₀ for this binding is 2.00‑30.0 ng/mL.
	Recombinant Human SorLA His-tag Protein SDS-PAGE. 2 μg/lane of Recombinant Human SorLA His-tag Protein (Catalog # 11083-LA) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 270-345 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11083-LA

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 1.00 mg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: SorLA

SorLA (sortilin-related receptor, LDLR class A repeats-containing), also called LR11 or SORL1, is a 250 kDa type I transmembrane glycoprotein of the Sortilin family of Vps10p-domain receptors (1, 2). SorLA is found in both intracellular and surface membranes in the central nervous system, and mediates trafficking of proteins such as the amyloid precursor protein (APP) (3-5). The 2214 amino acid (aa) human SorLA precursor includes a 28 aa signal sequence and a 53 aa furin-cleaved propeptide. The mature SorLA contains a vacuolar protein sorting 10 protein (Vps10p) homology domain, beta -propeller and epidermal growth factor (EGF) domains, a cluster of 11 complement-type repeat domains, six fibronectin type III repeats, a single transmembrane domain (TM), and a cytoplasmic domain (CT) (1, 6-7). Human SorLA shares 93% and 92% aa sequence identity with mouse and rat SorLA, respectively. SorLA is mainly expressed in nervous system, but is also found in non-neuronal tissues (8). Upon truncation, SorLA is able to bind multiple ligands among which are RAP, apolipoprotein E, and lipoprotein lipase, and facilitate both their endocytosis and sorting (1). The expression, translocation, and ectodomain shedding were shown to be induced by its ligand head activator peptide (HA) (9). SorLA has also been shown to be important in Alzheimer's disease through multiple functions, but especially through its ability to bind Amyloid Precursor Protein (4). It is involved in TrkB receptor and GFRa1 trafficking and further enhancement of BDNF and GDNF functions respectively (10, 11).

Item	Value
References	Jacobsen, L. et al. (2001) J. Biol. Chem. 276:22788. Wang, X. et al. (2011) Protein Expr. Purif. 77:224. Small, S.A. and S. Gandy (2006) Neuron. 52:15. Andersen, O.M. et al. (2005) Proc. Natl. Acad. Sci. U. S. A. 102:13461. Willnow, T.E. et al. (2008) Nat. Rev. Neurosci. 9:899. Herz J. (2001) Neuron 29:571. Yamazaki H. et al. (1996) J. Biol. Chem. 271:24761. Jacobsen L. et al. (1996) J. Biol. Chem. 271:31379. Hampe, W. et al. (2000) J. Cell Sci. 113:4475. Rohe, M. et al. (2013) PLoS One. 8:72164. Glerup, S. et al. (2013) Cell Rep. 3:186.
Long Name	Sortilin-related Receptor Containing LDLR Class A Repeats
Entrez Gene IDs	6653 (Human); 20660 (Mouse); 300652 (Rat)
Alternate Names	C11orf32, FLJ21930, FLJ39258, LR11, LR11LDLR relative with 11 ligand-binding repeats, LRP9, Low-density lipoprotein receptor relative with 11 ligand-binding repeats, SORL1, SorLA, SorLA-1SORLA, Sorting protein-related receptor containing LDLR class A repeats, chromosome 11 open reading frame 32, gp250, mosaic protein LR11, sortilin-related receptor, sortilin-related receptor, L(DLR class) A repeats containing, sortilin-related receptor, L(DLR class) A repeats-containing

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Recombinant Human SorLA His-tag Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Human SorLA His-tag Protein, CF

Scientific Data Examples for Recombinant Human SorLA His-tag Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: SorLA

Citations for Recombinant Human SorLA His-tag Protein, CF

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