Skip to main content

Recombinant Human Siglec-6/CD327 Fc Chimera Protein, CF

Catalog # 2859-SL | R&D Systems, Inc. a Bio-Techne Brand
Catalog #
Availability
Size / Price
Qty
Loading...
2859-SL-050

Key Product Details

Source

CHO

Accession #

Conjugate

Unconjugated

Applications

Binding Activity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Siglec-6/CD327 protein
Human Siglec-6
(Gln27-Val331)
Accession # NP_942142
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained: Gln27 predicted

Predicted Molecular Mass

60.3 kDa (monomer)

SDS-PAGE

80-95 kDa, reducing conditions

Activity

Measured by its ability to bind biotinylated Neu5Ac alpha2-6GalNAc alpha (sialyl-Tn). Patel, N. et al. (1999) The Journal of Biological Chemistry 274:22729.

Formulation, Preparation and Storage

2859-SL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Loading...
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Siglec-6/CD327

Siglecs (sialic acid binding Ig-like lectins) are I-type (Ig-type) lectins that belong to the Ig superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains (1 - 4). Eleven human Siglecs (Siglec-1 through 11) have been cloned and characterized. Within these eleven, there are at least two groups, one of which is termed the CD33-related group. CD33-related Siglecs include CD33/Siglec-3 and Siglec-5 through 11 (1, 3). To date, no Siglec has been shown to recognize any cell surface ligand other than sialic acid. This suggests that interactions with glycans containing this carbohydrate are important in mediating the biological functions of Siglecs. The cDNA of human Siglec-6 (also known as OB-BP1 and CD33L), encodes a putative 442 amino acid (aa) protein that contains a 15 aa signal peptide, a 321 aa extracellular region, a 21 aa transmembrane region (TM), and an 85 aa cytoplasmic tail (5, 6). The extracellular region contains one N-terminal V-type Ig-like domain followed by two Ig-like C2-type domains. The cytoplasmic domain has one immunoreceptor tyrosine-based inhibition motif (ITIM). At least three additional isoforms exist, all of which encode an additional 11 aa’s at the N-terminus, likely due to the utilization of an alternate start site. Two of the three isoforms also show splicing. One isoform shows a 16 aa in-frame deletion in the second C2-like domain, while the other shows a deletion of the TM and cytoplasmic region, thus potentially generating a soluble form (6 - 9). Siglec-6 is found on B cells and in placenta, and would seem to have a restricted specificity for the sialyl Tn antigen (6, 10).

References

  1. Crocker, P.R. and J. Zhang (2002) Biochem. Soc. Symp. 69:83.
  2. Crocker, P.R. and A. Varki (2001) Immunology 103:137.
  3. Crocker, P.R. (2002) Curr. Opin. Struct. Biol. 12:609.
  4. Powell, L.D. and A. Varki (1995) J. Biol. Chem. 270:14243.
  5. Takei, Y. et al. (1997) Cytogenet. Cell Genet. 78:295.
  6. Patel, N. et al. (1999) J. Biol. Chem. 274:22729.
  7. GenBank Accession # NP_942143.
  8. GenBank Accession #: NP_942142.
  9. GenBank Accession #: NP_001236.
  10. Crocker, P.R. and A. Varki (2001) Trends Immunol. 22:337.

Long Name

Sialic Acid Binding Ig-like Lectin 6

Alternate Names

CD327, CD33L1, OB-BP1, Siglec6

Entrez Gene IDs

946 (Human)

Gene Symbol

SIGLEC6

UniProt

Product Documents for Recombinant Human Siglec-6/CD327 Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Siglec-6/CD327 Fc Chimera Protein, CF

For research use only

Citations for Recombinant Human Siglec-6/CD327 Fc Chimera Protein, CF (3)

Showing  1 - 3 of 3 publications Showing All
Filter By:
Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human Siglec-6/CD327 Fc Chimera Protein, CF include:

There are no citations that match your criteria.

Showing  1 - 3 of 3 publications Showing All

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

÷
=
Loading...
Setting the standard in quality research reagents