Recombinant Human Semaphorin 6B Fc Chimera Protein, CF

Name: Recombinant Human Semaphorin 6B Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 2094-S6
Price: 460 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 2094-S6

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Key Product Details

Source:	NS0
Accession #:	Q9H3T3
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Human Semaphorin 6B Fc Chimera Protein, CF

Product Specifications for Recombinant Human Semaphorin 6B Fc Chimera Protein, CF

Source

Mouse myeloma cell line, NS0-derived human Semaphorin 6B protein

Human Semaphorin 6B (Leu26-Ser603) Accession # Q9H3T3	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Leu26

Predicted Molecular Mass

63.4 kDa (monomer)

SDS-PAGE

110-120 kDa, reducing conditions

Activity

Measured by its ability to cause collapse of chick embryonic dorsal root ganglia (DRG) neuron growth cones. 2.5-5.0 µg/mL of Recombinant Human Semaphorin 6B Fc Chimera causes >50% growth cone collapse in the presence of 10 ng/mL of Recombinant Human beta ‑NGF (Catalog # 256-GF).
Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2094-S6

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Semaphorin 6B

Semaphorin 6B is a member of the class 6 subfamily of semaphorins, a large, highly conserved family of signaling molecules that affect multiple processes including axon guidance, cell migration, synaptogenesis, and dendritic spine formation (1). The class 6 semaphorins are type I transmembrane glycoproteins that contain the characteristic extracellular beta propeller-structured N-terminal semaphorin (sema) domain and also an extracellular plexin-semaphorin-integrin (PSI) domain (2, 3). Additionally, the cytoplasmic domain of Semaphorin 6B contains a proline-rich sequence that has been shown to interact with the SH3 domain of the Src signaling protein (4). Human Semaphorin 6B has a molecular weight of approximately 120 kDa and shares 95% and 94% amino acid (aa) sequence identity with the mouse and rat orthologs, respectively. Full-length Semaphorin 6B is thought to form disulfide-linked homodimers (4). Alternative exon splicing creates a 492 aa isoform that may be secreted (Semaphorin 6B.1) and a 657 aa isoform with a shortened cytoplasmic tail (Semaphorin 6B.2) (5). Semaphorin 6B is highly expressed in heart and brain and is observed in most other tissues at lower levels (5). Semaphorin 6B has also been shown to be highly expressed in human gastric, breast, and glioblastoma cancer cells and is thought to be involved in tumor differentiation and metastasis (5-7). Interestingly, retinoic acid, which slows cancer cell growth, has been shown to down-regulate Semaphorin 6B expression (5, 6). It is hypothesized the retinoid X receptors (RXRs) form heterodimers with peroxisome proliferator-activated receptors (PPARs), which then bind to a putative peroxisome proliferator-responsive element (PPRE) in the SEMA6B promoter region (6, 8, 9). Semaphorin 6B has also been shown to mediate repulsion of mouse sympathetic ganglion axons and hippocampal mossy fibers via binding to the transmembrane protein Plexin A4 (10, 11).

Item	Value
References	Pasterkamp, R.J. and R.J. Giger (2009) Curr. Opin. Neurobiol. 19:263. Neufeld, G. et al. (2005) Front. Biosci. 10:751. Roney, K. et al. (2013) Protein Cell 4:17. Eckhardt, F. et al. (1997) Mol. Cell. Neurosci. 9:409. Correa, R.G. et al. (2001) Genomics 73:343. Murad, H. et al. (2006) Int. J. Oncol. 28:977. Ge, C. et al. (2013) J. Int. Med. Res. 41:284. Collet, P. et al. (2004) Genomics 83:1141. Murad, H. et al. (2011) Mol. Med. Rep. 4:575. Suto, F. et al. (2005) J. Neurosci. 25:3628. Tawarayama, H. et al. (2010) J. Neurosci. 30:7049.
Entrez Gene IDs	10501 (Human); 20359 (Mouse)
Alternate Names	SEM-SEMA-Y, SEMA-VIB, SEMAN, SEMAZ, Sema6B, Semaphorin 6B, UNQ1907/PRO4353, sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6B, semaphorin VIB, semaphorin Z, semaphorin-6B, semaphorin-6Ba

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Recombinant Human Semaphorin 6B Fc Chimera Protein, CF

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Product Specifications for Recombinant Human Semaphorin 6B Fc Chimera Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: Semaphorin 6B

Citations for Recombinant Human Semaphorin 6B Fc Chimera Protein, CF

Product Documents for Recombinant Human Semaphorin 6B Fc Chimera Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

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