Recombinant Human beta-NGF Protein

Name: Recombinant Human beta-NGF Protein
Brand: R&D Systems
SKU: 256-GF
Rating: 5 (3 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 256-GF

Citations (49)

(3)

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Key Product Details

Source:	NS0
Accession #:	CAA36832
Applications:	Bioactivity

View Full Technical Details for Recombinant Human beta-NGF Protein

Product Specifications for Recombinant Human beta-NGF Protein

Source	Mouse myeloma cell line, NS0-derived human beta-NGF protein Ser122-Ala241
Purity	>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ser122
Predicted Molecular Mass	13.5 kDa (monomer)
SDS-PAGE	13 kDa, reducing conditions
Activity	Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED₅₀ for this effect is 0.2‑2 ng/mL.

Scientific Data Examples for Recombinant Human beta-NGF Protein

	Recombinant Human beta-NGF Protein Bioactivity Recombinant Human beta -NGF (Catalog # 256-GF) stimulates cell proliferation of the TF-1 human erythroleukemic cell line. The ED₅₀ for this effect is 0.2‑2 ng/mL.
	Recombinant Human beta-NGF Protein SDS-PAGE 1 µg/lane of Recombinant Human beta -NGF was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 13 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

256-GF

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetic Acid with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

256-GF/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetic Acid.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: beta-NGF

NGF was initially isolated in the mouse submandibular gland as a 7S complex composed of three non-covalently linked subunits, alpha, beta, and gamma. Both the alpha and gamma subunits of NGF are members of the kallikrein family of serine proteases while the beta subunit, called beta -NGF or 2.5S NGF, exhibits all the biological activities ascribed to NGF. Recombinant human beta -NGF is a homodimer of two 120 amino acid polypeptides. The human protein shares approximately 90% homology at the amino acid level with both the mouse and rat beta -NGF and exhibits cross-species activity.

NGF is a well-characterized neurotropic protein that plays a critical role in the development of sympathetic and some sensory neurons in the peripheral nervous system. In addition, NGF can also act in the central nervous system as a trophic factor for basal forebrain cholinergic neurons. NGF has also been shown to have biological effects on non-neuronal tissues. NGF is mitogenic for a factor‑dependent human erythroleukemic cell line, TF-1. NGF has been found to increase the number of mast cells in neonatal rats and to induce histamine release from peritoneal mast cells. NGF will enhance histamine release and strongly modulate the formation of lipid mediators by basophils in response to various stimuli. NGF will also induce the growth and differentiation of human B lymphocytes as well as suppress apoptosis of murine peritoneal neutrophils. These results, taken together, suggest that NGF is a pleiotropic cytokine which, in addition to its neurotropic activities, may have an important role in the regulation of the immune system.

Item	Value
Long Name	beta Nerve Growth Factor
Entrez Gene IDs	4803 (Human); 18049 (Mouse); 310738 (Rat); 403402 (Canine); 103350089 (Rabbit)
Alternate Names	Beta nerve growth factor precursor, HSAN5, MGC161426, NGFB, NGFBMGC161428, beta-NGF, beta-nerve growth factor, betaNGF, nerve growth factor (beta polypeptide), nerve growth factor, beta subunit

Citations for Recombinant Human beta-NGF Protein (49)

Showing 1 - 10 of 49 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human beta-NGF Protein include:

Species: Human
Sample Types: Whole Cells
Applications: Differentiation

AK Holzer et al. (2022), Specific Attenuation of Purinergic Signaling during Bortezomib-Induced Peripheral Neuropathy In Vitro International Journal of Molecular Sciences, 23(7).
PMID: 35409095
Species: Horse
Sample Types: Cell Lysates
Applications: Control

S Nyström et al. (2022), The expression of nerve growth factor in healthy and inflamed equine chondrocytes analysed by capillary western immunoassay Research in Veterinary Science, 151(0):156-163.
PMID: 36029605
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

AT Regua et al. (2021), TrkA Interacts with and Phosphorylates STAT3 to Enhance Gene Transcription and Promote Breast Cancer Stem Cells in Triple-Negative and HER2-Enriched Breast Cancers Cancers, 13(10).
PMID: 34066153
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

B Sun et al. (2021), Regulation of host and virus genes by neuronal miR-138 favours herpes simplex virus 1 latency Nature Microbiology, 0(0).
PMID: 33558653
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

D Segelcke et al. (2021), Tmem160 contributes to the establishment of discrete nerve injury-induced pain behaviors in male mice Cell Reports, 37(12):110152.
PMID: 34936870
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

P Göttle et al. (2021), TLR4 Associated Signaling Disrupters as a New Means to Overcome HERV-W Envelope-Mediated Myelination Deficits Frontiers in Cellular Neuroscience, 15(0):777542.
PMID: 34887730
Species: Human
Sample Types: Whole Cells
Applications: Differentiation

AR Nickolls et al. (2020), Transcriptional Programming of Human Mechanosensory Neuron Subtypes from Pluripotent Stem Cells Cell Rep, 30(3):932-946.e7.
PMID: 31968264
Species: Rat
Sample Types: Whole Cells
Applications: cell cul

A Yoshida et al. (2020), Establishment of a simple one-step method for oligodendrocyte progenitor cell preparation from rodent brains J. Neurosci. Methods, 0(0):108798.
PMID: 32479973
Species: Human
Sample Types: Protein
Applications: Bioassay

JS Ebo et al. (2020), An in vivo platform to select and evolve aggregation-resistant proteins Nat Commun, 11(1):1816.
PMID: 32286330
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

N Uesato et al. (2020), Pharmacological Properties of JTE-952, an Orally Available and Selective Colony Stimulating Factor 1 Receptor Kinase Inhibitor Biol. Pharm. Bull., 43(2):325-333.
PMID: 32009119
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

KM Prautsch et al. (2020), Modulation of Human Adipose Stem Cells' Neurotrophic Capacity Using a Variety of Growth Factors for Neural Tissue Engineering Applications: Axonal Growth, Transcriptional, and Phosphoproteomic Analyses In Vitro Cells, 9(9).
PMID: 32839392
Species: Rat
Sample Types: Whole Cells
Applications: Cell Culture

B Xia et al. (2020), Mechanical stimulation of Schwann cells promote peripheral nerve regeneration via extracellular vesicle-mediated transfer of microRNA 23b-3p Theranostics, 10(20):8974-8995.
PMID: 32802175
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

B Xia et al. (2020), Mechanical stimulation of Schwann cells promote peripheral nerve regeneration via extracellular vesicle-mediated transfer of microRNA 23b-3p Theranostics, 10(20):8974-8995.
PMID: 32802175
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

KM Prautsch et al. (2020), Modulation of Human Adipose Stem Cells' Neurotrophic Capacity Using a Variety of Growth Factors for Neural Tissue Engineering Applications: Axonal Growth, Transcriptional, and Phosphoproteomic Analyses In Vitro Cells, 9(9).
PMID: 32839392
Species: Human

Applications: Bioassay

A Kizilyer et al. (2019), Inhibition of Tropomyosin Receptor Kinase A Signaling Negatively Regulates Megakaryopoiesis and induces Thrombopoiesis Sci Rep, 9(1):2781.
PMID: 30808933
Species: Human
Sample Types: Phage Supernates
Applications: ELISA (Capture)

AJ Poole et al. (2019), A C-terminal cysteine residue is required for peptide-based inhibition of the NGF/TrkA interaction at nM concentrations: implications for peptide-based analgesics Sci Rep, 9(1):930.
PMID: 30700786
Species: Human
Sample Types: Plasma
Applications: ELISA Standard

D Baumeister et al. (2019), No evidence for altered plasma NGF and BDNF levels in fibromyalgia patients Sci Rep, 9(1):13667.
PMID: 31541132
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

EP McCurdy et al. (2019), Promotion of Axon Growth by the Secreted End of a Transcription Factor Cell Rep, 29(2):363-377.e5.
PMID: 31597097
Species: Rat
Sample Types: Whole Tissue
Applications: Bioassay

B Xia et al. (2019), Extracellular Vesicles Derived From Olfactory Ensheathing Cells Promote Peripheral Nerve Regeneration in Rats Front Cell Neurosci, 13(0):548.
PMID: 31866834
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

S Park et al. (2019), Differentiation of Motor Neuron-Like Cells from Tonsil-Derived Mesenchymal Stem Cells and Their Possible Application to Neuromuscular Junction Formation Int J Mol Sci, 20(11).
PMID: 31159418
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

T Miladinovi et al. (2018), Functional effects of TrkA inhibition on system xC--mediated glutamate release and cancer-induced bone pain Mol Pain, 14(0):1744806918776.
PMID: 29761734
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

DR Gomez et al. (2018), Integrative proteomic and transcriptomic analysis provides evidence for TrkB (NTRK2) as a therapeutic target in combination with tyrosine kinase inhibitors for non-small cell lung cancer Oncotarget, 9(18):14268-14284.
PMID: 29581842
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

K Kirino et al. (2018), Efficient derivation of sympathetic neurons from human pluripotent stem cells with a defined condition Sci Rep, 8(1):12865.
PMID: 30150715
Species: Human
Sample Types: Silk Film
Applications: Bioassay

PM Deardorff et al. (2018), Modeling Diabetic Corneal Neuropathy in a 3D In Vitro Cornea System Sci Rep, 8(1):17294.
PMID: 30470798
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

JW Martin et al. (2018), Potent and rapid activation of tropomyosin-receptor kinase A in endometrial stromal fibroblasts by seminal plasma Biol. Reprod., 0(0).
PMID: 29518187
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

KC Chun et al. (2017), Immunohistochemical and immunocytochemical study of mechanoreceptors in anterior cruciate ligament reconstruction with the remnant-preserving technique using Achilles tendon allografts J Orthop Surg Res, 12(1):93.
PMID: 28615040
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Margriet A Huisman (2016), Isolation, expansion and neural differentiation of stem cells from human plucked hair: a further step towards autologous nerve recovery Cytotechnology, 68(5):1849-58.
PMID: 26702932
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

(2016), Nerve Growth Factor Attenuates Apoptosis and Inflammation in the Diabetic Cornea Invest. Ophthalmol. Vis. Sci, 57(15):6767-6775.
PMID: 27978558
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

(2016), Hierarchical CRMP2 posttranslational modifications control NaV1.7 function Proc. Natl. Acad. Sci. U.S.A, 0(0).
PMID: 27940916
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

(2016), Engineering the surface properties of a human monoclonal antibody prevents self-association and rapid clearance in vivo Sci Rep, 6(0):38644.
PMID: 27995962
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

S Delbary-Go et al. (2016), A novel inhibitor of p75-neurotrophin receptor improves functional outcomes in two models of traumatic brain injury Brain, 0(0).
PMID: 27084575
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

(2016), BMS-708163 and Nilotinib restore synaptic dysfunction in human embryonic stem cell-derived Alzheimer's disease models Sci Rep, 6(0):33427.
PMID: 27641902
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Park K et al. (2015), Effects of Reducing Suppressors of Cytokine Signaling-3 (SOCS3) Expression on Dendritic Outgrowth and Demyelination after Spinal Cord Injury. PLoS ONE, 10(9):e0138301.
PMID: 26384335
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Wan C et al. (2014), 2, 3, 7, 8-Tetrachlorodibenzo-P-dioxin (TCDD) induces premature senescence in human and rodent neuronal cells via ROS-dependent mechanisms. PLoS ONE, 9(2):e89811.
PMID: 24587053
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Schwab et al. (2014), Sensory neurons do not induce motor neuron loss in a human stem cell model of spinal muscular atrophy. PLoS ONE, 9(7):e103112.
PMID: 25054590
Species: Canine
Sample Types: Whole Cells
Applications: Bioassay

Gericota B et al. (2014), Canine epidermal neural crest stem cells: characterization and potential as therapy candidate for a large animal model of spinal cord injury. Stem Cells Transl Med, 3(3):334-45.
PMID: 24443004
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Ko J et al. (2014), Neuropeptides released from trigeminal neurons promote the stratification of human corneal epithelial cells. Invest Ophthalmol Vis Sci, 55(1):125-33.
PMID: 24327615
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Bowers P et al. (2013), Humanization of antibodies using heavy chain complementarity-determining region 3 grafting coupled with in vitro somatic hypermutation. J Biol Chem, 288(11):7688-96.
PMID: 23355464
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Delcroix GJ et al. (2010), EGF and bFGF pre-treatment enhances neural specification and the response to neuronal commitment of MIAMI cells. Differentiation, 80(4):213-27.
PMID: 20813449
Species: Porcine
Sample Types: Whole Cells
Applications: Bioassay

Fjord-Larsen L et al. (2010), Long-term delivery of nerve growth factor by encapsulated cell biodelivery in the Gottingen minipig basal forebrain. Mol. Ther., 18(12):2164-72.
PMID: 20664524
Schipper HS et al. (2010), A multiplex immunoassay for human adipokine profiling. Clin. Chem., 56(0):1320.
PMID: 20530731
Species: Canine
Sample Types: Whole Cells
Applications: Bioassay

Foveau B et al. (2009), Down-regulation of the met receptor tyrosine kinase by presenilin-dependent regulated intramembrane proteolysis. Mol. Biol. Cell, 20(9):2495-507.
PMID: 19297528
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Samah B et al. (2008), Neurotrophins modulate monocyte chemotaxis without affecting macrophage function. Clin. Exp. Immunol., 151(3):476-86.
PMID: 18190610
Paredes D et al. (2007), Effects of NGF and BDNF on baseline glutamate and dopamine release in the hippocampal formation of the adult rat. Brain Res., 1141(0):56-64.
PMID: 17292337
Species: N/A
Sample Types: N/A
Applications: Western Blot

Whittlesey KJ et al. (2006), Nerve growth factor expression by PLG-mediated lipofection. Biomaterials, 27(11):2477-86.
PMID: 16316681
Species: N/A
Sample Types: N/A
Applications: Western Blot

Young KR et al. (2006), Elicitation of immunity to HIV type 1 Gag is determined by Gag structure. AIDS Res. Hum. Retroviruses, 22(1):99-108.
PMID: 16438652
Species: Chicken
Sample Types: Whole Cells
Applications: Bioassay

Gallo G (2006), RhoA-kinase coordinates F-actin organization and myosin II activity during semaphorin-3A-induced axon retraction. J. Cell. Sci., 119(0):3413-23.
PMID: 16899819
Species: Chicken
Sample Types: Whole Cells
Applications: Bioassay

Adams DN et al. (2005), Growth cones turn and migrate up an immobilized gradient of the laminin IKVAV peptide. J. Neurobiol., 62(1):134-47.
PMID: 15452851
Species: Chicken
Sample Types: Whole Cells
Applications: Bioassay

Dolle L et al. (2003), Nerve growth factor overexpression and autocrine loop in breast cancer cells. Oncogene, 22(36):5592-601.
PMID: 12944907

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Recombinant Human beta-NGF Protein

Name: Anonymous

Application: Cell migration/motility

Verified Customer | Posted 05/19/2021
Recombinant Human beta-NGF Protein

Name: Anonymous

Application: Stem/Immune cell maintenance or differentiation

Verified Customer | Posted 05/20/2017
Recombinant Human beta-NGF Protein

Name: Pratima Rawat

Application: In my cell culture media to grow microglia cells

Reason for Rating: I found R&D growth factors quality much more superior than other sources.

Verified Customer | Posted 04/21/2017

Recombinant Human beta-NGF Protein

R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Human beta-NGF Protein

Scientific Data Examples for Recombinant Human beta-NGF Protein

Formulation, Preparation and Storage

Carrier Free

256-GF

Carrier

256-GF/CF

Carrier Free

Reconstitution Calculator

Background: beta-NGF

Citations for Recombinant Human beta-NGF Protein (49)

Customer Reviews for Recombinant Human beta-NGF Protein (3)

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Recombinant Human beta-NGF Protein

Recombinant Human beta-NGF Protein

Recombinant Human beta-NGF Protein

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