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Recombinant Human PDGF R alpha Protein, CF

Bio-Techne includes R&D Systems | Catalog # 322-PR/CF

A New Fc-tagged PDGF Ra is Available with ~10x better activity!
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322-PR-050/CF

Key Product Details

Source

NS0

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human PDGF R alpha protein
Gln24-Glu524

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Result not obtained: Gln24 predicted

Predicted Molecular Mass

56 kDa

SDS-PAGE

95-107 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human PDGF R alpha is immobilized at 2 µg/mL (100 µL/well), Recombinant Human PDGF-AB (Catalog # 222-AB) binds with an ED50 of 2-12 ng/mL.

Formulation, Preparation and Storage

322-PR/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PDGF R alpha

PDGF R alpha (platelet-derived growth factor receptor alpha) is a type I transmembrane glycoprotein in the class III subfamily of receptor tyrosine kinases (RTK) (1‑4). PDGF R alpha and PDGF R beta can form homo- or hetero-dimeric receptors when engaged by dimers of the PDGF family of growth factors, which include disulfide-linked homodimers of PDGF-A, B, C or D, or the heterodimer PDGF-AB that is mainly found in human platelets. While multiple in vitro ligand-receptor combinations have been identified, in vivo evidence indicates that PDGF R alpha primarily binds PDGF-AA and PDGF-CC, while PDGF R beta primarily binds PDGF-BB and probably PDGF-DD. Like all class III RTKs, the extracellular domain (ECD) of human PDGF R alpha (aa 24‑524) contains five immunoglobulin-like domains, while the intracellular region contains a split tyrosine kinase domain (aa 593‑954) (1‑4). Within the ECD, human PDGF R alpha shares 85%, 83%, 95%, 93%, and 88% aa sequence identity with mouse, rat, equine, canine and bovine PDGF R alpha respectively. PDGF R alpha autophosphorylates upon dimerization, activating signaling cascades in PI 3-kinase Ras-MAP kinase, and PLC-gamma pathways (1, 2). Signaling is down‑regulated by SHP-2 phosphatase activity and by receptor endocytosis and lysosomal degradation. PDGF R alpha is expressed at low levels in most mesenchymal cells, but is strongly expressed in oligodendrocyte, lung, skin and intestinal progenitor cells and induced by inflammation or growth in culture (1‑4). During development, mesenchymal cells expressing PDGF R alpha respond to local gradients of epithelially produced PDGF-AA or PDGF-CC during formation of the cranial and cardiac neural crest, retina, gonads, lung alveoli, intestinal villi, skin, hair follicles, skeleton, teeth, palate, and interstitial kidney mesenchyme (1, 5). Deletion of PDGF R alpha in mice severely impairs mesenchymal derivatives in both embryo and extraembryonic tissues, and high or low PDGF R alpha signaling in humans may result in spina bifida or cleft palate‑type malformations. Postnatally, PDGF R alpha is implicated in gliomas and fibrotic disorders of lung, heart and skin (scleroderma) (6‑8).

References

  1. Andrae, J. et al. (2008) Genes Dev. 22:1276.
  2. Heldin, C-H. and B. Westermark (1999) Physiol. Rev. 79:1283.
  3. Claesson-Welsh, L. et al. (1989) Proc. Natl. Acad. Sci. USA 86:4917.
  4. Matsui, T. et al. (1989) Science 243:800.
  5. Klinghoffer, R.A. et al. (2002) Dev. Cell 2:103.
  6. Martinho, O. (2009) Br. J. Cancer 101:973.
  7. Olson, L.E. and P. Soriano (2009) Dev. Cell 16:303.
  8. Baroni, S.S. et al. (2006) N. Engl. J. Med. 354:2667.

Long Name

Platelet-derived Growth Factor Receptor alpha

Alternate Names

CD140a, PDGFR alpha, PDGFRA

Entrez Gene IDs

5156 (Human); 18595 (Mouse)

Gene Symbol

PDGFRA

UniProt

Additional PDGF R alpha Products

Product Documents for Recombinant Human PDGF R alpha Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human PDGF R alpha Protein, CF

For research use only

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