Is there a linker between the PD-L1/B7-H1 protein and the His tag of Recombinant Human PD-L1/B7-H1 His-tag Protein, CF (Catalog # 9049-B7)?

The sequence for Recombinant Human PD-L1/B7-H1 His-tag Protein, CF (Catalog # 9049-B7) is Phe19-Thr239 (Accession # Q9NZQ7) fused directly to a C-terminal 6-His tag. This protein does not contain a linker sequence.

What are the differences between Recombinant Human PD-L1/B7-H1 His-tag Protein, CF (Catalog # 9049-B7) and Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF (Catalog # 156-B7)?

Recombinant Human PD-L1/B7-H1 Protein, CF (Catalog # 9049-B7) consists of (Phe19-Thr239) from Accession # Q9NZQ7. It has a His tag but no Fc chimera. Activity for 9049-B7 is measured in a binding assay with Recombinant Human PD‑1 Fc Chimera (Catalog # 1086-PD). Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF (Catalog 156-B7) consists of the same region (Phe19-Thr239), but instead of a His tag, it has a linker region and an Fc chimera. Activity for 156-B7 is assessed in a cell-based assay measuring IL-2 production by human T lymphocytes.

Recombinant Human PD-L1/B7-H1 His-tag Protein, CF

Name: Recombinant Human PD-L1/B7-H1 His-tag Protein, CF
Brand: R&D Systems
SKU: 9049-B7
Rating: 5 (12 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 9049-B7

Citations (6)

(12)

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Source:	HEK293
Accession #:	Q9NZQ7
Applications:	Binding Activity

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Product Specifications for Recombinant Human PD-L1/B7-H1 His-tag Protein, CF

Source	Human embryonic kidney cell, HEK293-derived human PD-L1/B7-H1 protein Phe19-Thr239, with a C-terminal 6-His tag
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Phe19
Predicted Molecular Mass	26 kDa
SDS-PAGE	31-41 kDa, reducing conditions
Activity	Measured by its binding ability in a functional ELISA. When Recombinant Human PD-L1/B7-H1 is immobilized at 0.025 µg/mL (100 µL/well), the concentration of Recombinant Human PD‑1 Fc Chimera (Catalog # 1086-PD) that produces 50% of the optimal binding response is approximately 0.05-0.3 μg/mL.

Scientific Data Examples for Recombinant Human PD-L1/B7-H1 His-tag Protein, CF

	Bioactivity of PD-L1 Protein When Recombinant Human PD-L1 / B7-H1 is immobilized at 0.025 µg/mL, it binds to Recombinant Human PD-1 Fc Chimera (Catalog # 1086-PD) with an ED₅₀ of 0.05-0.3 µg/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9049-B7

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: PD-L1/B7-H1

B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules (1). Mature human B7-H1 consists of a 220 amino acid (aa) extracellular domain (ECD) with two immunoglobulin-like domains, a 21 aa transmembrane segment, and a 31 aa cytoplasmic domain (2). Within the ECD, human B7-H1 shares 73% and 74% aa sequence identity with mouse and rat B7-H1, respectively. Alternative splicing generates additional isoforms that either lack the first Ig-like domain or are truncated within the second Ig-like domain (3). B7-H1 is expressed on inflammatory-activated immune cells including macrophages, T cells, and B cells (4-7), keratinocytes (8, 9), enothelial and intestinal epithelial cells (8, 10), as well as a variety of carcinomas and melanoma (11, 12). B7-H1 binds to T cell B7-1/CD80 and PD-1 (7, 8, 12-15). It suppresses T cell activation and proliferation (5, 8, 14, 16) and induces the apoptosis of activated T cells (11). It plays a role in the development of immune tolerance by promoting T cell anergy (7, 14) and enhancing regulatory T cell development (16). B7-H1 favors the development of anti-inflammatory IL-10 and IL-22 producing dendritic cells (5, 10) and inhibits the development of Th17 cells (16). In cancer, B7-H1 provides resistance to T cell mediated lysis, enhances EMT, and enhances the tumorigenic function of Th22 cells (6, 9, 12, 15).

Item	Value
References	Ceeraz, S. et al. (2013) Trends Immunol. 34:556. Dong, H. et al. (1999) Nat. Med. 5:1365. Frigola, X. et al. (2011) Clin. Cancer Res. 17:1915. Tamura, H. et al. (2001) Blood 97:1809. Chen, L. et al. (2007) J. Immunol. 178:6634. Kuang, D.-M. et al. (2014) J. Clin. Invest. 124:4657. Tsushima, F. et al. (2007) Blood 110:180. Mazanet, M.M. and C.C.W. Hughes (2002) J. Immunol. 169:3581. Cao, Y. et al. (2010) Cancer Res. 71:1235. Scandiuzzi, L. et al. (2014) Cell Rep. 6:625. Dong, H. et al. (2002) Nat. Med. 8:793. Azuma, T. et al. (2008) Blood 111:3635. Butte, M.J. et al. (2008) Mol. Immunol. 45:3567. Park, J.-J. et al. (2010) Blood 116:1291. Ritprajak, P. et al. (2010) J. Immunol. 184:4918. Herold, M. et al. (2015) J. Immunol. 195:3584.
Long Name	Programmed Death Ligand 1
Entrez Gene IDs	29126 (Human); 60533 (Mouse); 499342 (Rat); 574058 (Porcine); 484186 (Canine); 102145573 (Cynomolgus Monkey)
Alternate Names	Avelumab, B7-H, B7-H1, B7H1, B7H1PDCD1L1, CD274, CD274 antigenMGC142294, CD274 molecule, PD-L1, PD-L1B7 homolog 1, PDCD1L1, PDCD1LG1, PDCD1LG1MGC142296, PDL1, PDL1PDCD1 ligand 1, Programmed death ligand 1, programmed cell death 1 ligand 1

Citations for Recombinant Human PD-L1/B7-H1 His-tag Protein, CF (6)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human PD-L1/B7-H1 His-tag Protein, CF include:

Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay

R Le Biannic et al. (2022), Pyrazolones as inhibitors of immune checkpoint blocking the PD-1/PD-L1 interaction European Journal of Medicinal Chemistry, 236(0):114343.
PMID: 35429911
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

L Sun et al. (2022), PD-L1 promotes myofibroblastic activation of hepatic stellate cells by distinct mechanisms selective for TGF-beta receptor I versus II Cell Reports, 38(6):110349.
PMID: 35139382
ZH Wu et al. (2022), Development of the Novel Bifunctional Fusion Protein BR102 That Simultaneously Targets PD-L1 and TGF-beta for Anticancer Immunotherapy Cancers, 14(19).
PMID: 36230887
Species: Human
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance

C Geuijen et al. (2021), A human CD137ï¿½PD-L1 bispecific antibody promotes anti-tumor immunity via context-dependent T cell costimulation and checkpoint blockade Nature Communications, 12(1):4445.
PMID: 34290245
Species: Human
Sample Types: Recombinant Protein
Applications: Dimerization

JJ Park et al. (2021), Checkpoint inhibition through small molecule-induced internalization of programmed death-ligand 1 Nature Communications, 12(1):1222.
PMID: 33619272
Sample Types: Protein
Applications: Bioassay

Samit Chatterjee (2016), Rapid PD-L1 detection in tumors with PET using a highly specific peptide Biochem. Biophys. Res. Commun, 0(0).
PMID: 28025143

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Customer Reviews for Recombinant Human PD-L1/B7-H1 His-tag Protein, CF (12)

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Recombinant Human PD-L1/B7-H1 His-tag Protein, CF

Name: Anonymous

Application: In vitro bioactivity in cell culture

Verified Customer | Posted 10/24/2019
Recombinant Human PD-L1/B7-H1 His-tag Protein, CF

Name: Jenna Nguyen

Application: In vivo study

Verified Customer | Posted 01/11/2019
Recombinant Human PD-L1/B7-H1 His-tag Protein, CF

Name: Anonymous

Application: In vitro bioactivity in cell culture

Verified Customer | Posted 12/21/2018
Recombinant Human PD-L1/B7-H1 His-tag Protein, CF

Name: Anonymous

Application: Immunoassay Standard

Verified Customer | Posted 10/10/2018
Recombinant Human PD-L1/B7-H1 His-tag Protein, CF

Name: Anonymous

Application: Immunoassay Standard

Verified Customer | Posted 08/26/2018
Recombinant Human PD-L1/B7-H1 His-tag Protein, CF

Name: Anonymous

Application: Binding assay/Protein-protein interaction

Verified Customer | Posted 04/12/2018
Recombinant Human PD-L1/B7-H1 His-tag Protein, CF

Name: Anonymous

Application: SDS-PAGE Control

Verified Customer | Posted 03/30/2018
Recombinant Human PD-L1/B7-H1 His-tag Protein, CF

Name: Anonymous

Application: SDS-PAGE Control

Verified Customer | Posted 03/15/2018
Recombinant Human PD-L1/B7-H1 His-tag Protein, CF

Name: Anonymous

Application: SDS-PAGE Control

Verified Customer | Posted 03/11/2018
Recombinant Human PD-L1/B7-H1 His-tag Protein, CF

Name: Amit Kumar

Application: SDS-PAGE Control

Verified Customer | Posted 12/18/2017
Recombinant Human PD-L1/B7-H1 His-tag Protein, CF

Name: Anonymous

Application: Binding assay/Protein-protein interaction

Verified Customer | Posted 11/27/2017
Recombinant Human PD-L1/B7-H1 His-tag Protein, CF

Name: Anonymous

Application: Binding assay/Protein-protein interaction

Verified Customer | Posted 11/18/2017

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FAQs for Recombinant Human PD-L1/B7-H1 His-tag Protein, CF

Is there a linker between the PD-L1/B7-H1 protein and the His tag of Recombinant Human PD-L1/B7-H1 His-tag Protein, CF (Catalog # 9049-B7)?

The sequence for Recombinant Human PD-L1/B7-H1 His-tag Protein, CF (Catalog # 9049-B7) is Phe19-Thr239 (Accession # Q9NZQ7) fused directly to a C-terminal 6-His tag. This protein does not contain a linker sequence.
What are the differences between Recombinant Human PD-L1/B7-H1 His-tag Protein, CF (Catalog # 9049-B7) and Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF (Catalog # 156-B7)?

Recombinant Human PD-L1/B7-H1 Protein, CF (Catalog # 9049-B7) consists of (Phe19-Thr239) from Accession # Q9NZQ7. It has a His tag but no Fc chimera. Activity for 9049-B7 is measured in a binding assay with Recombinant Human PD‑1 Fc Chimera (Catalog # 1086-PD). Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF (Catalog 156-B7) consists of the same region (Phe19-Thr239), but instead of a His tag, it has a linker region and an Fc chimera. Activity for 156-B7 is assessed in a cell-based assay measuring IL-2 production by human T lymphocytes.

Recombinant Human PD-L1/B7-H1 His-tag Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Complete Your Research

Complete Your Experiment

Product Specifications for Recombinant Human PD-L1/B7-H1 His-tag Protein, CF

Scientific Data Examples for Recombinant Human PD-L1/B7-H1 His-tag Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: PD-L1/B7-H1

Citations for Recombinant Human PD-L1/B7-H1 His-tag Protein, CF (6)

Customer Reviews for Recombinant Human PD-L1/B7-H1 His-tag Protein, CF (12)

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Recombinant Human PD-L1/B7-H1 His-tag Protein, CF

Recombinant Human PD-L1/B7-H1 His-tag Protein, CF

Recombinant Human PD-L1/B7-H1 His-tag Protein, CF

Recombinant Human PD-L1/B7-H1 His-tag Protein, CF

Recombinant Human PD-L1/B7-H1 His-tag Protein, CF

Recombinant Human PD-L1/B7-H1 His-tag Protein, CF

Recombinant Human PD-L1/B7-H1 His-tag Protein, CF

Recombinant Human PD-L1/B7-H1 His-tag Protein, CF

Recombinant Human PD-L1/B7-H1 His-tag Protein, CF

Recombinant Human PD-L1/B7-H1 His-tag Protein, CF

Recombinant Human PD-L1/B7-H1 His-tag Protein, CF

Recombinant Human PD-L1/B7-H1 His-tag Protein, CF

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