Recombinant Human Noggin Fc Chimera Protein, CF

Name: Recombinant Human Noggin Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 3344-NG

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 3344-NG

Citations (21)

Product Datasheet / COA / SDS

New Version Available - 6057-NG/CF

A New and Improved Noggin is Now Available! The new protein has ~2 fold better activity, higher purity, and no tag! Earn double rewards for reviews

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Setting the standard in quality research reagents

Key Product Details

Source:	NS0
Accession #:	Q13253
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Human Noggin Fc Chimera Protein, CF

Product Specifications for Recombinant Human Noggin Fc Chimera Protein, CF

Source

Mouse myeloma cell line, NS0-derived human Noggin protein

Human Noggin (Gln28-Cys232) Accession # Q13253	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

No results obtained: Gln28 predicted

Predicted Molecular Mass

49.6 kDa (monomer)

SDS-PAGE

58-62 kDa, reducing conditions

Activity

Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED₅₀ for this effect is 0.100-0.400 µg/mL in the presence of 30 ng/mL of Recombinant Human BMP‑4 (Catalog # 314-BP).

Scientific Data Examples for Recombinant Human Noggin Fc Chimera Protein, CF

	Bioactivity of Human Noggin Recombinant human Noggin Fc chimera (3344-NG) inhibits recombinant human BMP-4-induced alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. The ED₅₀ for this effect is 0.100-0.400 µg/mL in the presence of 30 ng/mL of recombinant human BMP-4 (314-BP).

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3344-NG

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Noggin

Noggin is a secreted homodimeric glycoprotein that is an antagonist of bone morphogenetic proteins (BMPs) (1, 2). Human Noggin cDNA encodes a 232 amino acid (aa) precursor protein; cleavage of a 19 aa signal peptide generates the 213 aa mature protein which contains an N-terminal acidic region, a central basic heparin‑binding segment and a C-terminal cysteine-knot structure (2). Secreted Noggin probably remains close to the cell surface due to its binding of heparin‑containing proteoglycans (3). Noggin is very highly conserved among vertebrates, such that mature human Noggin shares 99%, 99%, 98%, 97% and 89% aa sequence identity with mouse, rat, bovine, equine and chicken Noggin, respectively. Noggin binds some BMPs such as BMP-4 with high affinity and others such as BMP-7 with lower affinity, antagonizing BMP bioactivities by blocking epitopes on BMPs that are needed for binding to both type I and type II receptors (2, 4).

During embryogenesis, Noggin antagonizes specific BMPs at defined times during neural tube, somite and cardiomyocyte growth and patterning (5-7). During skeletal development, Noggin prevents chondrocyte hyperplasia, thus allowing proper formation of joints (4). Mutations within the cysteine-knot region of human Noggin are linked to multiple types of skeletal dysplasias that result in apical joint fusions (8). Noggin is expressed in defined areas of the adult central nervous system and peripheral tissues such as lung, skeletal muscle and skin (1). During culture of human embryonic stem cells (hESC) without feeder layers or conditioned medium, but with addition of FGF basic, addition of Noggin to antagonize BMP activity allows hESC to maintain their undifferentiated, pluripotent state (9, 10). In differentiation protocols, Noggin has been used to create neural crest stem cells from induced pluripotent stem cells (11).

Because of its importance in the development of tissues, regenerative medicine utilizes Noggin to generate cells for intestinal tissues or organoids in vitro (12). Noggin is also an important factor for stimulating bone development and has neuroprotective effects in early stages of spinal cord injury (13, 14). Expression of Noggin can help contain or reduce metastatic lesions by limiting BMP signaling, making it a therapeutic option for cancer treatment (15). Noggin has been used to create bladder cancer organoids that can serve as a tissue model in preclinical testing of chimeric antigen receptor (CAR)-T-cell immunotherapy (16).

Item	Value
References	Valenzuela, D.M. et al. (1995) J. Neurosci. 15:6077. Groppe, J. et al. (2002) Nature 420:636. Paine-Saunders, S et al. (2002) J. Biol. Chem. 277:2089. Brunet, L. J. et al. (1998) Science 280:1455. McMahon, J. A. et al. (1998) Genes Dev. 12:1438. Itsykson, P. et al. (2005) Mol. Cell. Neurosci. 30:24. Yuasa, S. et al. (2005) Nat. Biotechnol. 23:607. Gong, Y. et al. (1999) Nat. Genet. 21:302. Xu, R.-H. et al. (2005) Nat. Methods 2:185. Wang, G. et al. (2005) Biochem. Biophys. Res. Commun. 330:934. Abe, R. et al. (2021) J. Rural. Med. 16:143. Kim, S. et al. (2022) Nat. Commu. 13:1692. Malijauskaite, S. et al. (2021) Cytokines Growth Factor Rev. 60:76. Al-Sammarraie, N. et al. (2022) Neural Regen Res. 18:492. Davis, H. et al. (2016) Cytokines Growth Factor Rev. 27:81. Yu, L. et al. (2021) Clin. Transl. Immunology 10:e1248.
Entrez Gene IDs	9241 (Human); 18121 (Mouse)
Alternate Names	NOG, Noggin, SYM1, SYNS1, SYNS1A, symphalangism 1 (proximal), synostoses (multiple) syndrome 1

Citations for Recombinant Human Noggin Fc Chimera Protein, CF (21)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human Noggin Fc Chimera Protein, CF include:

Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

S Martin et al. (2022), Oligodendrocyte differentiation alters tRNA modifications and codon optimality-mediated mRNA decay Nature Communications, 13(1):5003.
PMID: 36008413
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

D Yang et al. (2022), Modeling human multi-lineage heart field development with pluripotent stem cells Cell Stem Cell, 29(9):1382-1401.e8.
PMID: 36055193
Species: Human
Sample Types: Transfected Whole Cells
Applications: Bioassay

T Wakimizu et al. (2022), SHOX2 refines the identification of human sinoatrial nodal cell population in the inï¿½vitro cardiac differentiation Regenerative Therapy, 21(0):239-249.
PMID: 36092505
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Y Aghazadeh et al. (2021), Microvessels support engraftment and functionality of human islets and hESC-derived pancreatic progenitors in diabetes models Cell Stem Cell, 0(0).
PMID: 34480863
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

KC Allan et al. (2020), Non-canonical Targets of HIF1a Impair Oligodendrocyte Progenitor Cell Function Cell Stem Cell, 0(0).
PMID: 33091368
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

SH Chung et al. (2019), Differentiation of Retinal Glial Cells From Human Embryonic Stem Cells by Promoting the Notch Signaling Pathway Front Cell Neurosci, 13(0):527.
PMID: 31849614
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture

M Matjusaiti et al. (2019), Reprogramming of Fibroblasts to Oligodendrocyte Progenitor-like Cells Using CRISPR/Cas9-Based Synthetic Transcription Factors Stem Cell Reports, 13(6):1053-1067.
PMID: 31708478
Species: Xenopus
Sample Types: In Vivo
Applications: In Vivo

P Scerbo et al. (2017), Lineage commitment of embryonic cells involves MEK1-dependent clearance of pluripotency regulator Ventx2 Elife, 6(0).
PMID: 28654420
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Christine Kestens et al. (2016), BMP4 Signaling Is Able to Induce an Epithelial-Mesenchymal Transition-Like Phenotype in Barrett's Esophagus and Esophageal Adenocarcinoma through Induction of SNAIL2. PLoS ONE, 0(0):1932-6203.
PMID: 27191723
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Guezguez A et al. (2014), Modulation of stemness in a human normal intestinal epithelial crypt cell line by activation of the WNT signaling pathway. Exp Cell Res, 322(2):355-64.
PMID: 24534551
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

Prabakar K et al. (2012), Generation of glucose-responsive, insulin-producing cells from human umbilical cord blood-derived mesenchymal stem cells. Cell Transplant, 21(6):1321-39.
PMID: 22195604
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Kelly OG et al. (2011), Cell-surface markers for the isolation of pancreatic cell types derived from human embryonic stem cells. Nat. Biotechnol., 29(8):750-6.
PMID: 21804561
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Kelly MA et al. (2009), Signaling hierarchy regulating human endothelial cell development. Arterioscler. Thromb. Vasc. Biol., 29(5):718-24.
PMID: 19213939
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Gil JE et al. (2009), Vitronectin promotes oligodendrocyte differentiation during neurogenesis of human embryonic stem cells. FEBS Lett., 583(3):561-7.
PMID: 19162023
Species: Bovine
Sample Types: Whole Cells
Applications: Bioassay

Yao Y et al. (2008), High-density lipoproteins affect endothelial BMP-signaling by modulating expression of the activin-like kinase receptor 1 and 2. Arterioscler. Thromb. Vasc. Biol., 28(12):2266-74.
PMID: 18948634
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Elkabetz Y et al. (2008), Human ES cell-derived neural rosettes reveal a functionally distinct early neural stem cell stage. Genes Dev., 22(2):152-65.
PMID: 18198334
Species: Xenopus
Sample Types: Whole Cells
Applications: Bioassay

Wen Z et al. (2007), BMP gradients steer nerve growth cones by a balancing act of LIM kinase and Slingshot phosphatase on ADF/cofilin. J. Cell Biol., 178(1):107-19.
PMID: 17606869
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

McLean AB et al. (2007), Activin a efficiently specifies definitive endoderm from human embryonic stem cells only when phosphatidylinositol 3-kinase signaling is suppressed. Stem Cells, 25(1):29-38.
PMID: 17204604
Species: Chicken
Sample Types: In Vivo
Applications: In Vivo

Knobloch J et al. (2007), Thalidomide induces limb deformities by perturbing the Bmp/Dkk1/Wnt signaling pathway. FASEB J., 21(7):1410-21.
PMID: 17283219
Species: Chicken
Sample Types: In Ovo
Applications: In Ovo

Bonafede A et al. (2006), BMPs restrict the position of premuscle masses in the limb buds by influencing Tcf4 expression. Dev. Biol., 299(2):330-44.
PMID: 17005177
Species: Porcine
Sample Types: Whole Cells
Applications: Bioassay

Zeng L et al. (2006), Multipotent adult progenitor cells from swine bone marrow. Stem Cells, 24(11):2355-66.
PMID: 16931778

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