Recombinant Human Neogenin His Tagged Protein, CF

Name: Recombinant Human Neogenin His Tagged Protein, CF
Brand: R&D Systems
SKU: 8607-NE
Price: 460 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 8607-NE

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Setting the standard in quality research reagents

Key Product Details

Source:	HEK293
Accession #:	Q92859
Applications:	Bioactivity

View Full Technical Details for Recombinant Human Neogenin His Tagged Protein, CF

Product Specifications for Recombinant Human Neogenin His Tagged Protein, CF

Source	Human embryonic kidney cell, HEK293-derived human Neogenin protein Ala34-Met1104, with a C-terminal 6-His tag
Purity	>90%, by SDS-PAGE with silver staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ala34
Predicted Molecular Mass	118 kDa
SDS-PAGE	130-153 kDa, reducing conditions
Activity	Measured by its binding ability in a functional ELISA. When Recombinant Human Neogenin is immobilized at 1.5 µg/mL (100 µL/well), the concentration of Recombinant Mouse Netrin‑1 (Catalog # 1109-N1) that produces 50% of the optimal binding response is approximately 15-75 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8607-NE

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 250 μg/mL in PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Neogenin

Neogenin is a type I transmembrane protein belonging to the Ig superfamily. It is composed of an extracellular segment containing four Ig-like C2-type domains and six Fibronectin type III domains (1). Neogenin has a molecular weight of approximately 190 kDa, and the extracellular domain of the human protein shares 91% and 93% amino acid sequence identity with the mouse and rat orthologs, respectively (1). Four different isoforms are produced from alternative splicing of human NEO1. Neogenin is widely expressed in adult human tissues with the highest levels being observed in skeletal muscle and pancreas (1). It is also ubiquitously expressed in both neuronal and non-neuronal tissues of the developing mouse embryo (2). Neogenin is a multifunctional cell-surface receptor that binds to members of the Netrin, Repulsive Guidance Molecule (RGM) and Bone Morphogenetic Protein (BMP) families (3-5). It has also been shown to interact with members of the UNC5 family and in certain instances, associate with CDO as a co-receptor (6-8). Neogenin appears to be involved in the regulation of multiple developmental processes including development of the central nervous system (CNS), myogenesis, angiogenesis, and formation of mammary glands (4, 5, 7-9). During CNS development, Neogenin regulates neural tube closure, neuronal differentiation, and cell survival (4, 5, 7). It also mediates Netrin-1-dependent attraction and RGM-A-dependent repulsion of growing axons (4, 5, 7, 10). Additionally, Neogenin binding to RGM and Netrin proteins regulates cell-cell adhesion, cell migration, tissue organization, and adult neurogenesis (4, 7, 11). Neogenin is thought to be involved in tumorgenesis and cancer cell invasiveness in brain and gastric cancers (12-14).

Item	Value
References	Meyerhardt, J.A. et al. (1997) Oncogene 14:1129. Keeling, S.L. et al. (1997) Oncogene 15:691. Hagihara, M. et al. (2011) J. Biol. Chem. 286:5157. Tian, C. and J. Liu (2013) Mol. Reprod. Dev. 80:700. Severyn, C.J. et al. (2009) Biochem. J. 422:393. van den Heuvel, D.M. et al. (2013) PLoS ONE 8:e55828. De Vries, M. and H.M. Cooper (2008) J. Neurochem. 106:1483. Krauss, R.S. et al. (2005) J. Cell Sci. 118:2355. Srinivasan, K. et al. (2003) Dev. Cell 4:371. de Castro, F. (2003) News Physiol. Sci. 18:130. O’Leary, C.J. et al. (2015) Stem Cells 33:503. Milla, L.A. et al. (2014) Int. J. Cancer 134:21. Akino, T. et al. (2014) Cancer Res. 74:3716. Kim, S.J. et al. (2014) Oncotarget 5:3386.
Entrez Gene IDs	4756 (Human); 18007 (Mouse)
Alternate Names	HsT17534, IGDCC2DKFZp547B146, Immunoglobulin superfamily DCC subclass member 2, NEO, NEO1, NGN, NGNDKFZp547A066, Neogenin, neogenin (chicken) homolog 1, neogenin 1, neogenin homolog 1

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Recombinant Human Neogenin His Tagged Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Human Neogenin His Tagged Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: Neogenin

Citations for Recombinant Human Neogenin His Tagged Protein, CF

Product Documents for Recombinant Human Neogenin His Tagged Protein, CF

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COA

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